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Biomedical subjects

T Kusaka

Publications and source records attributed to T Kusaka.

107 records · Page 6Linked to original sources
PubMed1970

Elongation of fatty acids in Mycobacterium tuberculosis.

Cell-free extracts of the H37Ra strain of Mycobacterium tuberculosis contain a soluble enzyme system which catalyzes an elongation reaction of long-chain fatty acids. The predominant reaction involves the addition of a single C(2) unit to the acceptor fatty acid; the elongation takes place exclusively at the carboxyl end of the acceptor molecule. The endogenous acceptor lipid can be removed by solvent extraction of the enzyme system. The lipid-depleted enzyme can be fully reactivated with external acyl coenzyme A, after which elongation with acetyl coenzyme A takes place. The elongation reaction is avidin-insensitive and does not require adenosine triphosphate. Reduced nicotinamide adenine dinucleotide is the source of reducing equivalent, whereas reduced nicotinamide adenine dinucleotide phosphate is without effect.

Carbon Isotopes↗
PubMedDate not supplied

Fatty acid synthesizing enzyme activity of cultured Mycobacterium lepraemurium.

In comparing the specific activity of enzymes pertaining to the biosynthesis of fatty acids in crude extracts of cultivated M. lepraemurium and M. smegmatis, it was found that: 1. The activity of acetyl CoA carboxylase of the former organism was undetectable and that of de novo fatty acid synthetase was too weak to measure exactly, under the condition used, whereas both activities of the latter organism were comparable to those already reported by other authors. 2. The activity of acetyl CoA dependent acyl CoA elongation system of M. lepraemurium was relatively high and close to that of M. smegmatis. 3. The activities of acetyl CoA synthetase and acyl CoA synthetase of M. lepraemurium were moderately lower than those of M. smegmatis. The relation between this peculiar fatty acid synthesizing enzyme system of M. lepraemurium and its extremely sluggish growth is discussed.

Acetate-CoA Ligase↗