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Biomedical subjects

T Kuroyanagi

Publications and source records attributed to T Kuroyanagi.

At least 37 records · Page 2Linked to original sources
PubMed1981

The in vitro development of immunoglobulin producing cells from the human bone marrow null lymphocyte.

The distribution of human bone marrow lymphocyte population was studied on twenty normal adults. The distribution of bone marrow lymphocytes were as follows: null lymphocytes 79.4%, E-RFC 8.9%, Thy+ lymphocytes 0.4%, Tgamma 0.9%, Tmi 1.4%, SmIg+ cells 7.8%, EAC-RFC 11.7%, EA-RFC 6.2%, and K cells 2.7%. Bone marrow null lymphocytes were intermediate in their size between small lymphocytes and lymphoblasts. The presence of a lot of short microvilli on null lymphocytes was demonstrated by scanning electron microscopic examinations. The bone marrow null lymphocytes had no cytoplasmic Ig. When the bone marrow null lymphocytes were mixed-lymphocytes-cultured with allogeneic T lymphocytes and then stimulated with PWM, there developed Ig producing cells. Differences between pre-B cells and bone marrow null lymphocytes were discussed.

Antibody-Producing Cells↗
PubMed1979

A case report of the immunodysplasia syndrome and heavy chain disease associated with subacute bacterial endocarditis.

A 36-year-old man was admitted to Saitama Medical School Hospital, because of a remittent fever which had continued for approximately 6 months, hepatosplenomegaly and lymphadenopathy. He had direct Coombs' test positive auto-immune hemolytic anemia associted with subacute bacterial endocarditis (SBE). The lymphnode demonstrated focal diffuse proliferation of immunoblasts and arborizing vessels with a few small germinal centers, which resembled histological features of the immunoblastic lymphadenopathy. The immunochemical analysis revealed the presence of free IgG Fc fragments in serum. From the above results the patient was diagnosed as immunodysplasia syndrome (IDS) and heavy chain disease (HCD) associated with SBE. It was suggested that the chronic antigenic stimulation due to SBE might have some role in the mechanism of the development of the IDS and HCD in our patient.

Adult↗
PubMed1978

The presence of new permeability factor in serum of patients with systemic lupus erythematosus and its significance.

We have found a new permeability factor in serum of patients with systemic lupus erythematosus. It is non-dialyzable, heat stable, and long acting as compared to histamine or bradykinin which is short acting. It has no esterolytic nor smooth muscle contracting activities. It is not inhibited by anti-histamine drugs, soy bean trypsin inhibitor, DFP or Cl esterase inhibitor. It is independent of the kallikrein system. It has the common antigenicity with IgG Fc fragments. Its approximate molecular weight is about 55,000. So we tentatively call this permeability factor IgG-PF. Intravenous injections of HGG-anti-HGG immune complex, which has been formed by antigen-antibody reactions in 20 times antigen excess, into rats resulted in no immune complex nephritis. However, intravenous injections of HGG-anti-HGG immune complex with IgG-PF resulted in immune complex nephritis in rats. The above immune complex nephritis was inhibited by administrations of sulfapyridine but not by administrations of anti-histamine. These results indicate that IgG-PF plays some roles in the mechanism of immune complex nephritis.

Animals↗
PubMed1977

A new method for evaluating an increased general capillary permeability in patients.

The difference between total plasma volume determined with a substance which escapes from vascular beds in the presence of an increase of general capillary permeability and that determined with a substance which is confined to blood even in the presence of an increased capillary permeability may reflect the degree of an increase of general capillary permeability. The total plasma volume was determined by simultaneous injections of 131I-HSA and 51Cr tagged red cells. The capillary permeability was evaluated by calculating the difference (deltaTPV) between total plasma volume determined with 131I-HSA and that determined with 51Cr tagged red cell. deltaTPV in patients with systemic lupus erythematosus, idiopathic thrombocytopenic purpura, chronic active hepatitis, liver cirrhosis and subacute baterial endocarditis was larger than that of controls, averaging 204ml/m2, 178 ml/m2, 82 ml/m2, 131 ml/m2 and 179 ml/m2, respectively. The increase of deltaTPV was considered to indicate the increase of capillary permeability in these patients. A permeability increasing factor was present in serum of patients with an elevated deltaTPV. There was a significant correlation between deltaTPV and the titer of serum capillary permeability increasing factor in these patients.

Adult↗
PubMed1976

Fibrin degradation products in renal diseases.

Levels of serum fibrin degradation products (FDP) were determined in patients with acute nephritis, chronic nephritis, lupus nephritis and toxemia of pregnancy by the passive hemagglutination inhibition test. Serum FDP levels were less than 10 mug/ml in normal control adults, averaging 3.2 +/- 1.2 mug/ml. The incidence of serum FDP positive patients (more than 10 mug/ml) in those with acute nephritis, chronic nephritis, lupus nephritis and toxemia of pregnancy was 28%, 73%, 100% and 100%, respectively. Their serum FDP levels averaged 8.4 +/- 5.6 mug/ml, 16.0 +/- 5.9 mug/ml, 21.4 +/- 7.6 mug/ml and 35 mug/ml, respectively. Plasma fibrinogen levels, prothrombin time, partial thromboplastin time, euglobulin lysis time and platelet counts were within normal limits in serum FDP positive patients with renal diseases, indicating that there was no severe disseminated intravascular coagulation. All FDP positive patients with renal diseases of immunological origin demonstrated the deposition of fibrin within glomeruli with complement and immunoglobulin deposits. However, FDP positive patients with toxemia of pregnancy demonstrated fibrin depositions within glomeruli without complement and immunoglobulin deposits. FDP D fragments of urine from lupus nephritis patients showed no changes in immunoelectrophoretic patterns by heat treatment, indicating that urine FDP was derived from secondary fibrinolysis.

Acute Disease↗
PubMed1975

Effects of heterologous anti-erythrocyte antibodies on the generation cycle of erythroblasts in rats.

An injection of anti-rat erythrocyte rabbit serum or its 7S globulin fraction into rats resulted in immunohemolytic anemia associated with a positive anti-gloublin test examined with anti-rabbit gammaglobulin serum. However, an injection of 19S globulin fraction from the anti-erythrocyte serum failed to cause immunohemolytic anemia, although it induced a transient decrease in red cell count. These results indicate that 7S antibodies are responsible for the induction of anti-globulin test positive, experimental immunohemolytic anemia. The ferrokinetic and stathmokinetic studies revealed the prolongation of generation time of basophilic and polychromatic erythroblasts in rats injected with anti-erythrocyte serum or its 7S globulin fraction. The results from in vitro [3H] thymidine incorporation experiments also confirmed this prolongation. The analysis of in vivo [3H] thymidine labeling of erythroblasts in rats which were given the 7S GLOBULIN ANTIBODIES SUGGESTED THE PROLONGATION OF THE G1 time of the erythroblasts. These results suggest that the effects of anti-erythrocyte antibodies on the generation cycle of erythroblasts are to prolong their G1 time and keep them dormant.

Anemia, Hemolytic↗