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Biomedical subjects

T Kurimura

Publications and source records attributed to T Kurimura.

At least 91 records · Page 5Linked to original sources
PubMed1983

A clustering outbreak of hand, foot, and mouth disease caused by Coxsackie virus A10.

A clustering outbreak of hand, foot, and mouth disease (HFMD) occurred from July, 1981 to January, 1982 in Matsue City and Gotsu City, Shimane Prefecture. Thirty-seven patients with clinical HFMD were virologically and serologically examined, and Coxsackie virus A10 (CA10) was isolated in 18 patients from vesicles (7/16), throat-swabs (9/31) and feces (6/7). During the period, no CA16 or enterovirus 71 were isolated from HFMD patients or from other diseases such as pharyngitis, febrile diseases, and aseptic meningitis. Serological diagnosis was performed employing an African green monkey kidney cell (AG-1)-adapted CA10 which demonstrated cytopathogenic effects on the cells. Paired sera from seven patients including three cases in which isolation failed showed a significant increase of neutralizing antibody titer against CA10. Finally, an etiological diagnosis was made in 21 out of 37 patients with clinical HFMD. This is the first report of a clustering outbreak of HFMD caused by CA10 in Japan.

Antibodies, Viral↗
PubMed1983

Comparison of enzyme-linked immunosorbent assay, electron microscopy, and reversed passive haemagglutination for detection of human rotavirus in stool specimens.

An enzyme-linked immunosorbent assay (ELISA) using microplates as solid phase, rabbit antiserum against human rotavirus Wa strain as catching antibody, and the same reagent labeled with beta-D-galactosidase as conjugate, has been developed for detection of human rotavirus antigen(s) in stool specimens from patients with acute gastroenteritis. The limit of detection of purified human rotavirus by ELISA was 15.6 ng/ml (1.56 ng/well) of viral protein. The sensitivities of ELISA, electron microscopy, and the reversed passive haemagglutination method (ROTA-CELL) were compared. ELISA was more sensitive than electron microscopy and the reversed passive haemagglutination method. The ELISA blocking assay was useful for detection of an antibody response to human rotavirus in paired sera from children in two institutions during outbreaks of rotavirus gastroenteritis.

Antibodies, Viral↗
PubMed1983

Interferon-alpha in serum and carcinomatous pleural effusion after repeated intrapleural injections of antitumor agents.

Pleural effusions and sera of two patients with lung cancer were tested after intrapleural injection of OK-432 as an anticancer drug for IFN-alpha activity by biological assay and for IFN-alpha as an antigen by radioimmunoassay. The titers by radioimmunoassay were fairly consistent with those by biological assay, but were usually higher. In Case 1, IFN-alpha was observed fairly early after administration of OK-432 and only in pleural effusions. In Case 2, induction of IFN-alpha at low level was observed late after the first administration of OK-432 both in the pleural effusion and serum and was detected only by radioimmunoassay.

Adenocarcinoma↗
PubMed1983

Enzyme immunoassay of HBeAg employing beta-D-galactosidase.

An enzyme-linked immunosorbent assay (ELISA) system for hepatitis B e antigen (HBeAg) was developed employing beta-D-galactosidase conjugated with antibody to HBeAg (anti-HBe) and using m-maleimidobenzoyl-N-hydroxysuccinimide ester as the coupling reagent. The experimental conditions for quantitative assay of HBeAg were determined. The presence of rheumatoid factor in test sera did not affect the results. This assay system is more sensitive than the micro-Ouchterlony method and as sensitive as radioimmunoassay. The use of beta-D-galactosidase for ELISA in the field of virology is recommended.

Galactosidases↗
PubMed1982

Mitogenic activity in human embryonic fibroblasts early after infection by human cytomegalovirus.

We have characterized the nonspecific lymphocyte stimulation by extracts of human cytomegalovirus-infected human embryonic fibroblasts. Cell extracts prepared at 5 h postinfection (early extract) and 72 h postinfection (late extract) were both highly mitogenic in lymphocyte preparations from adult blood, cord blood, and rabbit blood. Maximum stimulation of the lymphocytes was observed on day 3 after the addition of early or late extract under optimal conditions. Early extract stimulated both the E-rosetting and the EAC-rosetting subpopulations of human lymphocytes. The mitogenic activity appeared before 5 h postinfection and was fairly stable at 30 degrees C for 5 h.

Animals↗
PubMed1981

In situ electron microscopical observation of cells infected with herpes simplex virus.

Transport and release of herpes simplex virus (HSV) in an African green monkey kidney cell line (CV-1) was followed by electron microscopy for up to 24 h p.i. Transmission electron microscopy and scanning electron microscopy were employed. For the former approach, electron microscopical autoradiography of whole cultured cells and in situ thin section techniques were used. The following new observations were made. (1) Except in peripheral parts of the cells, where the cytoplasmic membrane could make a ruffling movement relatively freely, virus particles were found only on the dorsal surface of the cell and not on the surface facing the substratum. By observation of thin section in situ, it was confirmed that the virus particles within intracytoplasmic vacuoles were apparently released by a reverse phagocytic process from the cell surface adjacent to microvillus projections. (2) Progeny virus particles in the nucleus moved to the cell surface within 2 h after maturation.

Animals↗
PubMed1980

DNA synthesis and multinucleation of mouse cells infected with SV40 in the presence of cytochalasin B.

DNA synthesis and nuclear division of mouse cells, BALB/3T3, infected with SV40 were studied and were compared with those of SV40-transformed mouse cells, mKS-A TU-7. Both SV40-infected and SV40-transformed cells behaved similarly in the presence of cytochalasin B and differently from normal noninfected cells, BALB/3T3. The chemical inhibited cytokinesis of all the cell groups but the nuclear division was inhibited only in the case of non-infected BALB/3T3. With SV40-infected BALB/3T3, multinucleation occurred with the increase of input m.o.i. by SV40. SV40-infected BALB/3T3 could enter the second S phase after release from double thymidine block in the presence of cytochalasin B, while BALB/3T3 could not enter the second S phase. In bi- or multinucleated cells of SV40-infected BALB/3T3, asynchrony of DNA synthesis among nuclei in a cell was evident, as was the case with mKS-A TU-7.

Animals↗
PubMed1980

Comparative studies on the inhibitory effects of interferon on various strains of herpes simplex viruses in vitro.

The sensitivities to human leukocyte interferon of 10 strains of Herpes simplex virus (HSV) type 1 and 3 strains of type 2 were compared. All the strains were sensitive to interferon, although their sensitivities were less than that of vesicular stomatitis virus (VSV). There was no significant difference in the sensitivities to interferon of HSV type 1 and type-2 or among different strains of a given type of HSV. Nor was there any difference between the sensitivities of 5-iodo-2'-deoxyuridine (IDU)-sensitive and resistant strains isolated from the same patients. These results suggest that interferon should be useful in therapy of HSV infection.

Cell Line↗
PubMed1979

Differences in antibody responses in varicella, herpes zoster and after vaccination.

Difference in antibody responses between varicella and herpes zoster was reconfirmed by complement fixation (CF) test and by platelet aggregation (PA) test. Rise in PA antibody level was observed only in patients with herpes zoster, but not in patients with varicella, while the increase in CF antibody was demonstrated in both types of the diseases. Among 235 healthy children, CF antibody was detected in 42 children and out of these 25 also had PA antibody. Sera of 116 individuals who were vaccinated with a live vaccine comprised of varicella-herpes zoster virus (VZV) were tested for CF and PA antibodies. Within six months after vaccination, 81.3 per cent of the vaccinees demonstrated the rise in CF antibody alone, and only one vaccinee possessed low level of PA antibody. Six months to 1 year after vaccination, CF antibody in 12 out of 13 vaccinees fell to undetectable level. Later than 1 year after vaccination, 9 out of 28 vaccinees possessed CF antibody. Two out of these 9 vaccinees also had PA antibody. The PA antigens prepared from viruses isolated either from a varicella patient or a herpes zoster patient responded similarly to the sera of herpes zoster patients, but no response was detected with sera of varicella patients. PA and CF antibody titers of herpes zoster patients showed a high degree of correlation.

Adolescent↗
PubMed1979

Seasonality of birth in sporadic cretinism.

The seasonal distribution of birth dates of 31 patients with sporadic cretinism due to thyroid dysgenesis was analyzed in Osaka area for 14 years. The incidence was statistically high in the summer months. A hypothesis that some environmental factors such as viral infection may cause the disease is proposed.

Birth Rate↗
PubMed1979

Induction of SV40-related transplantation immunity in mice by BK virus-transformed cells.

The reactivity of tumor-specific transplantation antigens (TSTAs) induced by SV40 and BK virus (BKV) was studied in Balb/Ccr mice with SV40-transformed tumor cells (mKSA-4AC). The mice, which had received BKV-transformed mouse or hamster cells, were highly resistant to the challenge with mKSA-4AC. The mKSA-rejected animals, however, were as susceptible as non-immunized ones to the subsequent challenge with Moloney sarcoma virus (Kirsten)-transformed cells. The result shows that BKV-transformed cells have a TSTA immunologically related closely to that induced by SV40.

Animals↗
PubMed1979

Epidemiological and virological studies on outbreaks of acute gastroenteritis associated with rotavirus in primary schools in Osaka.

There have been three recent outbreaks of acute gastroenteritis in primary schools in Osaka prefecture caused by rotavirus: one in a school (TA) in April, 1974 and two in two other schools (TE and K) in May 1975. The morbidity from the disease was 2.4--15.8% for all age groups in the schools, and 20.1--34.1% for a certain age group. The disease lasted for 4 (K) to 14 days (TE). The first cases in schools TA and TE were followed by successive cases. The main clinical symptoms were higher frequencies of diarrhea (68--71.4%) and fever (78%) than in winter vomiting disease. There was no difference in the incidences of the disease in boys and girls. From observation on the disease in individual families, the incubation period seemed to be 3 days. Using paired sera and Neonatal Calf Diarrhea Virus (NCDV) as antigen, positive seroconversion was demonstrated by the complement fixation test and marked seroconversion by the indirect fluorescent antibody technique. With these techniques, specific antibody to NCDV was detectable in the sera from an early stage of illness. Rotavirus was found on electron microscopic examination of some fecal specimens of patients in TA, but not in those of patients in TE or K, although adenovirus was isolated from one patient. A serological survey of healthy children aged 0 to 12 showed that rotavirus is a common virus in Osaka.

Acute Disease↗
PubMed1979

Analysis of herpes simplex virus isolated from patients with recurrent herpes keratitis exhibiting "treatment-resistance" to 5-iodo-2'-deoxyuridine.

Four strains of herpes simplex virus (HSV) were isolated from two patients with recurrent herpes keratitis who failed to respond to 5'-iodo-2'-deoxyuridine (IDU) treatment. Two of the four isolates were highly resistant to IDU in cell culture and the other two isolates were more susceptible to IDU than an HSV-1 laboratory strain. From each patient, an IDU-resistant and an IDU-susceptible virus was isolated. All 4 isolates possessed the ability to induce the thymidine kinase (TK) activity in cell lines lacking that activity. All the isolates were type 1 HSV, since the filamentous structures, recognized as a biological marker of type 2 HSV, were not observed in infected cells.

Animals↗
PubMed1978

Field experiments on live attenuated Japanese encephalitis virus vaccine for swine.

The efficacy of a live attenuated Japanese encephalitis virus (JEV) vaccine was examined in swine under conditions where natural infection could occur. The pigs immunized with the vaccine produced antibodies within one week after vaccination, and the antibody was retained until the end of the experiment, i.e. 36 days. However, the antibody titers in this group were lower than that in control group naturally infected with JEV. No virus was isolated from the five vaccinated pigs, but virus was isolated from all four untreated control pigs after natural infection, i.e., viremia was detected in all these animals. The duration of viremia in control pigs varied from one to four days. From these findings, it is concluded that immunization of swine with live attentuated JEV vaccine is useful in control of Japanese encephalitis (JE) in humans and some susceptible domestic animals.

Animals↗