Search PubMed⌕ Search

Biomedical subjects

T Kurihara

Publications and source records attributed to T Kurihara.

At least 397 records · Page 22Linked to original sources
PubMed1977

The use of non-aqueous chloroform/methanol extraction for the delipidation of brain with minimal loss of enzyme activities.

When freeze-dried brain was extracted at -4-0degrees C with dry chloroform/methanol (2:1 v/v), four of the five enzymes examined were recovered in the diethyl ether-washed residue without inactivation. By contrast, extraction with chloroform/methanol (2:1 v/v) in the presence of water destroyed activities of all the enzymes examined. The amounts of major lipids extracted were similar whether extraction was done in the absence or presence of water. The study was carried out with special interest in 2':3'-cyclic nucleotide 3'-phosphodiesterase (EC 3.1.4.37), which is firmly bound to the membrane structures of brain white matter.

2',3'-Cyclic-Nucleotide Phosphodiesterases↗
PubMed1977

Familial progressive bulbar-spinal muscular atrophy: case report with muscle biopsy study.

A case of familial progressive bulbar and spinal muscular atrophy was presented. The patient was a 59-year-old male with chief complaints of gait disturbance and nasal voice. His illness started at the age of 39 and very slowly progressed over 20 years. The clinical symptoms and signs were characterized by muscle weakness and atrophy due to lower motor neuron disease in the brain stem below the lower pons and the spinal cord. The electromyograms and muscle biopsy findings are basically neurogenic. In spite of the bulbar signs, the course of the disease is extremely slow. The diagnostic criteria was proposed after reviewing eight other cases reported in the literature.

Adult↗
PubMed1976

Electron microscopic studies of the T-system--the effects of hyperosmolar glycerol and dantrolene sodium on mammalian muscles.

Electron microscopic studies of the T-systems in mammalian muscles were performed to investigate the effects of hyperosmolar glycerol solution and dantrolene sodium. The results indicate that in mammalian muscles the terminal cisterns of the sarcoplasmic reticulum seem to be the site of morphological changes which occur when the muscle specimens were transferred from the hypertonic glycerol solution to isotonic washing solution. When the muscle specimen was further exposed to dantrolene solution, the above changes were intensified. The changes of the terminal cisterns of SR were characterized by prominent vacuolar changes and the T-tubules were pressed and narrowed by those vacuoles secondarily. The glycerol and dantrolene technique did not cause any electron microscopic changes in the sarcolemma, myofibrils, nor Z-discs, and selective changes in the T-system, i.e. at the terminal cisterns of SR were demonstrated. This technique, excitation-contraction uncoupling, abolishes isometric twitch tension up to 99.7% and serves for the purpose of prolonged intracellular recordings of mammalian muscles without movement induced artifacts.

Animals↗