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T Kuramata

Publications and source records attributed to T Kuramata.

At least 37 records · Page 2Linked to original sources
PubMed1982

[A study on the disc sensitivity test for fosfomycin (author's transl)].

Susceptibilities to fosfomycin of 216 strains of 28 bacterial species were determined by the 2-fold agar dilution method in parallel with the diameter of inhibition zone by the single-disc method, under the experimental condition establish by Kanazawa. The experiments demonstrated significant correlation between MIC by the dilution method and diameter of inhibition zone in each of conventional assay of the over-night (about 16 hours) incubation, delayed assay (about 24 hours incubation), thus confirming applicability of the single-disc assay for fosfomycin. Analysis of the data obtained by using fosfomycin disc containing 200 micrograms revealed the primary regression equation to be: D (diameter, mm) = 42.73-13.85 log MIC (microgram/ml) in conventional assay, D = 56.51-18.18 log (microgram/ml) in delayed assay, respectively. The range of variations in MICs estimated from the diameter of inhibition zone by the disc test was then calculated in comparison with that in MIC determined by the 2-fold agar dilution assays, as reference for the experimental errors which may be involved in the estimation of MIC of fosfomycin by the single-disc assay.

Anti-Bacterial Agents↗
PubMed1982

[A study of the disc sensitivity test for cephradine].

Susceptibilities of 189 strains of 28 bacterial species to cephradine were determined by the 2-fold agar dilution method in parallel with the diameter of inhibition zone by the single-disc method, under the experimental condition established by Kanazawa. The experiments demonstrated significant correlation between MIC by the dilution method and diameter of inhibition zone in each of conventional assay of the over-night (about 16 hours) incubation, delayed assay (about 24 hours incubation), and rapid assay (after 3-4 or 5-6 hours incubation), thus confirming applicability of the single-disc assay for cephradine. Analysis of the data obtained by using cephradine disc containing 30 micrograms revealed the primary regression equation to be: D (diameter, mm) = 28.5-11.2 log MIC (microgram/ml) in conventional assay, D = 35.1-14.6 log MIC (microgram/ml) in delayed assay, D = 18.0-5.41 log MIC (microgram/ml) in 3-4 hours rapid assay and D = 23.2-8.33 log MIC (microgram/ml) in 5-6 6 hours rapid assay, respectively. The range of variations in MICs estimated from the diameter of of inhibition zone by the disc test was then calculated in comparison with that in MIC determined by the 2-fold agar dilution assays, as reference for the experimental errors which may be involved in the estimation of MIC of cephradine by the single-disc assay.

Bacteria↗
PubMed1981

[A study on the disc sensitivity test for amikacin (author's transl)].

Susceptibilities to amikacin of 153 strains of 27 bacterial species were determined by the 2-fold agar dilution method in parallel with the diameter of inhibition zone by the single-disc method. The experiments demonstrated significant correlation between MIC by the dilution method and diameter of inhibition zone in each of conventional assay of the over-night (about 16 hours) incubation, delayed assay (about 24 hours incubation), and rapid assay (after 3-4 or 5-6 hours incubation), thus confirming applicability of the single-disc assay for amikacin. Analysis of the data obtained by using amikacin disc containing 30 micrograms revealed the primary regression equation to be: D (diameter, mm)=23.73-8.55 log MIC (micrograms/ml) in conventional assay, D=29.84-11.53 log MIC (microgram/ml) in delayed assay, D=16.47-4.53 log MIC (microgram/ml) in 3-4 hours rapid assay, and D=19.57-6.19 log MIC (micrograms/ml) in 5-6 hours rapid assay, respectively. The range of variations in MICs estimated from the diameter of inhibition zone by the disc test was then calculated in comparison with that in MIC determined by the two-fold agar dilution assays, as reference for the experimental errors which may be involved in the estimation of MIC of amikacin by the single-disc assay.

Amikacin↗
PubMed1976

[Drug sensitivity of Yersinia enterocolitica and Yersinia pseudotuberculosis].

Drug-sensitivity of 70 strains of Y. enterocolitica and 24 of Y. pseudotuberculosis including reference strains and isolates from men or animals were determined by the agar plate-dilution method. All the strains of Y. enterocolitica were relatively resistant to penicillins and cephalosporins, while all of Y. pseudotuberculosis were sensitive to both of the agents. Most of the strains of both species excluding 13 resistant ones were sensitive to streptomycin. The resistant strains, however, were sensitive to other aminoglycoside antibiotics, i.e. kanamycin, paromomycin, gentamicin, tobramycin, dibekacin and amikacin. All of the strains were sensitive to chloramphenicol. Most of the strains excluding 3 resistant ones were highly sensitive to tetracycline. The resistant strains were also resistant to doxycycline and methacycline but not to minocycline. All of them were resistant to erythromycin, to lincomycin and to novobiocin. Most of them excluding 7 resistant strains were sensitive to sulfisoxazole. All of them were sensitive to nalidixic acid, piromidic acid, and also to dihydroxymethylfuratrizine. A new synthetic agent, fosfomycin showed a relatively wide range of activity to the strains, but none of resistant strains were noticed. These resistant strains were all found in the isolates from men and animals but not in reference strains. Among these resistant strains, resistance patterns were as follows; TC, SM, SA in 3 of Y. enterocolitica, SM, SA in 3 of Y. enterocolitica and in 1 of Y. pseudotuberculosis, and SM in 3 of the both species, respectively. As for polypeptide antibiotics, polymyxin B and colistin, some strains of the both species showed an uncontinous susceptibility, i.e. inhibited growth at a certain lower concentration but growth at a higher concentration.

Amikacin↗
PubMed1976

[Studies on the disc method for the determination of bacterial sensitivity to ribostamycin (author's transl)].

The MICs of ribostamycin (RSM) were determined by the two-fold serial agar-dilution method of 109 bacterial strains of 20 species. The diameters of inhibition zones of these bacterial strains by the 50 mug RSM disc were also measured. The relation between the MIC and the diameter of the inhibition zone was found to be expressed as a primary regression line in all cases of the conventional method (cultured for about 16 hours), delayed assay method (cultured for about 24 hours) and rapid methods (5 approximately 6 hours and 3 approximately 4 hours culture methods). Thus, it was confirmed that the single-disc method can be employed for the susceptibility test of RSM. Subsequently, variations of MICs obtained by the disc-diffusion method were compared with those obtained by the serial agar-dilution method.

Anti-Bacterial Agents↗
PubMed1975

[Transmission of drug-resistance through conjugation in Yersinia enterocolitica and Yersinia pseudotuberculosis. (II). With special reference to streptomycin resistant strains isolated from men and domestic animals (author's transl)].

Seven strains of Y. enterocolitica, of which 2 strains being resistant to SM, TC and SA, 2 to SM and SA 3 to SM, and 3 strains of Y. pseudotuberculosis resistant to SM have been isolated from men and domestic animals. Tests were carried out with these 10 isolates for transfer of the drug resistance by bacterial conjugation, and evidence was obtained for transfer of resistance from 1 SM-resistant Y. enterocolitica and 2 Y. pseudotuberculosis strains to the sensitive E. coli K-12. Moreover, secondary transfer of this resistance to sensitive strains of the donor species Y. enterocolitica and Y pseudotuberculosis was also demonstrated. The findings indicate the spontaneous occurrence of organisms carrying the resistance transfer factor, R(Sm), among the populations of these two species in the natural environment.

Animals↗
PubMed1975

[Studies on the disc method for the determination of bacterial sensitivity to sulfobenzylpenicillin (author's transl)].

The MIC''s of sulfobenzylpenicillin (SB-PC) were determined by the two-fold serial agar-dilution method for 108 bacterial strains of 21 species. The diameters of inhibition zones of these bacterial strains by the 30 mug and 200 mug SB-PC discs were also measured. The relation between the MIC and the diameter of the inhibition zone was found to be expressed as a primary regression line in all cases of the conventional method (cultured for about 16 hours), delayed assay method (cultured for about 24 hours) and rapid methods (5 to approximately 6 hours and 3 to approximately 4 hours culture m ethods). Thus, it was confirmed that the single-disc method can be employed for the susceptibility test of SB-PC. Subsequently, variations of MICs obtained by the disc-diffusion method were compared with those obtained by the serial agar-dilution method.

Escherichia coli↗