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Biomedical subjects

T Kumamoto

Publications and source records attributed to T Kumamoto.

At least 163 records · Page 9Linked to original sources
PubMed1985

Influence of diet on the development of gastrin- and somatostatin-like immunoreactivity in the stomach of rats.

The effects of changes in diet on antral gastrin-like immunoreactivities and antral and corporal somatostatin-like immunoreactivities were studied in three groups of young male rats. Group 1 rats were weaned physiologically, group 2 rats received only breast milk, and group 3 rats were fed milk until 20 days of age and then were switched abruptly to laboratory chow. In group 1, antral gastrin-like immunoreactivity and somatostatin-like immunoreactivity increased gradually from 13 days of age to adults. In group 2, antral gastrin-like immunoreactivity did not increase, and antral and corporal somatostatin-like immunoreactivity increased until 20 and 25 days of age, respectively. In group 3, there were three- to fivefold increases of antral gastrin-like immunoreactivity and somatostatin-like immunoreactivity after changing to laboratory chow. Dietary changes had no effect on the concentration of corporal somatostatin-like immunoreactivity. These results suggest that the developmental patterns of gastric gastrin-like immunoreactivity and somatostatin-like immunoreactivity are closely related to diet; laboratory chow may play an important role in the development of these gut hormones.

Animals↗
PubMed1985

Morphological studies of peripheral nerves and skeletal muscles of an adult case with adrenoleukomyeloneuropathy.

A 53-year-old patient with adrenoleukomyeloneuropathy, showing symptoms of mental deterioration, cerebellar ataxia, spastic tetraparesis, and polyneuropathy, was reported. The myelinated fiber densities were decreased in the anterior spinal root and sciatic nerve. Most of the myelinated fibers showed segmental demyelination and a few fibers showed axonal degeneration. Electron microscopy showed numerous lipid inclusions in the cytoplasm of the Schwann cells. The muscles showed neurogenic changes. Numerous lipid bodies and intracytoplasmic crystalline arrays were found in the sarcolemma.

Adrenoleukodystrophy↗
PubMed1983

[Electrophoresis of proteins of muscle fibers with rimmed vacuoles].

This is the first report on the analysis of the proteins in the single muscle fibers with rimmed vacuoles. A new method was developed to analyse single muscle fibers which had been histologically characterized in the freeze-dried sections of muscles. Muscle biopsy was performed in three patients with distal myopathy and one patient with Kearns-Sayre-like syndrome associated with rimmed vacuoles (Table 1). Ten (30 micron thick) frozen serial sections were dried according to Lowry and Passonneau, and further five consecutive sections were cut and stained with usual histochemical methods including Gomori trichrome and NADH-TR staining. Each muscle fiber in the freeze-dried sections could be identified with the corresponding one in the stained sections, and it was possible to determine whether the fiber had rimmed vacuoles or not (Fig. 2). Particular fibers were identified in the consecutive freeze-dried sections and cut out from three sections with no contamination of the interstitial tissue, and they were subjected to SDS gel electrophoresis. Resultant gel patterns showed substantial increase of 55 k dalton band, the extent of which was much more larger in that of the fiber with rimmed vacuoles than that without vacuoles (Fig. 3). This feature was common to all of the gels from four patients. A portion containing a number of muscle fibers of a freeze-dried section from each of the four patients and the two controls was subjected to two-dimensional gel electrophoresis (2-D). Of 55 k dalton proteins, three spots around the isoelectric point of alpha-actin were found to be increased (Fig. 4).(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1982

Distal myopathy: histochemical and ultrastructural studies.

In three familial cases and one sporadic case of late-onset distal myopathy, muscle wasting started in the distal portions of the lower extremities. The most striking change seen by light microscopy was the appearance of rimmed vacuoles. These were presumed to be autophagic, because they were found by electron microscopy to contain membranous lamellar structures and other heterogenous materials enclosed by a limiting membrane. On the other hand, lysosomal activity was markedly increased in skeletal muscle. In 6% to 22% of affected muscle fibers there were acid phosphatase-positive granules deep in the sarcoplasm, whereas control muscles had no such granules. The degenerative process in distal myopathy may be different from that in other muscular dystrophies.

Acid Phosphatase↗
PubMed1982

The peripheral neuropathy in De Sanctis-Cacchione syndrome. Histological, ultrastructural, and morphometric studies.

Histological, ultrastructural, and morphometric studies were performed on nerve and muscle biopsies from three patients with de Sanctis-Cacchione syndrome. Sural nerves showed marked loss of the myelinated fibers, in proportion to decrease in nerve conduction velocities and in inverse proportion to the severity of the clinical symptoms, which were related to the survival length. The larger fibers were involved earlier and more markedly than the smaller. The unmyelinated fibers were also decreased in number. Electron-microscopic studies showed the presence of primary degeneration of myelin sheaths or Schwann cells. Muscle biopsies showed grouping of type I and type II fibers in all three patients. Therefore, peripheral nerve involvement in de Sanctis-Cacchione syndrome was suggested to result from chronic degeneration of the neuronal cells and Schwann cells.

Adolescent↗
PubMed1982

Oculopharyngeal muscular dystrophy and distal myopathy. Intrafamilial difference in the onset and distribution of muscular involvement.

A family is reported which included a patient with a variant form of oculopharyngeal muscular dystrophy. The patient's son suffered from infantile muscular dystrophy with a distal distribution in the lower extremities and no oculopharyngeal symptoms. Case 1, the father, showed blepharoptosis, but no limitation of ocular movements. Case 2, the son, showed early onset of weakness and more rapid progression of muscle involvement than the father. In both patients EMG, muscle biopsies and elevated serum CPK indicated the myopathic nature of the disorder. A muscle biopsy specimen in Case 2 showed abundant rimmed vacuoles and abnormal filaments 13-19 nm in diameter in the sarcoplasm, usually reported to occur in inclusion body myositis. The findings indicate that oculopharyngeal muscular dystrophy and distal myopathy are related in their etiology and distal myopathy and inclusion body myositis are regarded as variant forms of the same disease.

Adult↗
PubMed1981

A new myopathy with tubulomembranous inclusions.

A muscle biopsy performed on a 16-year-old boy with progressive myopathy revealed hitherto unrecognized peculiar inclusions which consisted of 3 types of structures. The first type consisted of laminated tubulomembranous structures and most of the inclusions belonged to this type. The lamellae were regularly spaced with a periodicity of 8.5--9 nm and curving a little, and were observed as concentric lamellae according to the plane of sectioning. The second type of inclusions consisted of curvifilamentous material. The third type had the appearance of moderately electron-dense granular material surrounded by a single unit membrane. The origin and nature of these inclusions is obscure, but the diagnosis of some kind of storage myopathy was suspected in this case.

Adolescent↗