Serum from a grand multiparous Inuit (Eskimo) woman, who is HL-A identical but stimulatory in MLC with cells of her husband, contains strong ABCIL reactivity. This serum is operationally monospecific against a cell membrane determinant on lymphocytes, but not platelets, of some unrelated persons. Cells of those who are ABCIL positive with this serum always give a lower MLC stimulation index compared with their MLC responsiveness to cells that are ABCIL negative. This serum also contains good MLC inhibitory activity but only in those one way MLC reactions in which it reacts, by ABCIL, with the stimulating cells. It is postulated that the ABCIL activity is directed against a common MLC factor on lymphocytes.

Antigen-Antibody Reactions↗

PubMed1975

Cell-mediated immunity to Herpes simplex in humans: lymphocyte cytotoxicity measured by 51-Cr release from infected cells.

We assessed cell-mediated immunity to herpes simplex virus type 1 antigen in patients suffering from recurrent cold sores and in a series of healthy controls. Paradoxically, all those subject to recurrent herpetic infections had, without exception, evidence of cell-mediated immunity to herpes antigens. This was demonstrated by lymphocyte transformation and specific 51Cr release from infected human amnion cells after incubation with peripheal blood mononuclear cells. Where performed, skin tests with herpes antigen were also positive. In addition, serum from these patients specifically sensitized herpesvirus-infected cells to killing by nonimmune, control mononuclear cells. These tests were negative in the control patients except in a few cases, and it is suggested that these latter may be the asymptomatic herpesvirus carriers previously recognized or that they may have experienced a genital infection.

Amnion↗

PubMed1974

Serological detection of mixed lymphocyte culture identity between cells that differ by one HL-A haplotype.

Antibody mediated cell dependent immune lympholysis (ABCIL), an extremely sensitive serological technique for detecting tissue sensitization, was used in a family segregation study. Two serums capable of demonstrating ABCIL were used to identify members of the family who differed by one HL-A haplotype but whose cells did not stimulate in mixed lymphocyte culture. Absorption study of one serum indicated that the ABCIL reaction of that serum was directed against a factor, independent of HL-A, that might be responsible for lymphocyte stimulation in mixed lymphocyte culture. Thus the ABCIL technique may be used to detect lymphocyte-defined gene products.

Antigens↗

PubMed1974