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Biomedical subjects

T Kotani

Publications and source records attributed to T Kotani.

At least 217 records · Page 12Linked to original sources
PubMed1985

Characterization of a monoclonal antibody to hog thyroid peroxidase and its use for immunohistochemical localization of the peroxidase in the thyroid gland.

A monoclonal antibody (30.1.2) to hog thyroid peroxidase was produced, purified, and characterized. The IgG of 30.1.2 formed an immune complex with the peroxidase in a 1:2 or 1:1 molar ratio depending on the IgG to antigen ratio in the incubation mixture. Immune complex formation did not inhibit the peroxidase activity, which was actually activated 2-fold in the 1:1 complex. Studies of the binding of the conjugate of the IgG or its Fab' with horseradish peroxidase to untreated and acetone-treated thyroid microsomes showed that the IgG conjugate could bind to only a very small portion of the total binding sites (thyroid peroxidase) present in untreated microsomes even after prolonged incubation. The binding of the Fab' conjugate to untreated microsomes, on the other hand, increased as the incubation time was increased, reaching 40% of the total sites after 20 h of incubation. These findings indicated that thyroid peroxidase is localized on the inner surface of the microsomal membranes and that the Fab' conjugate, but not the IgG conjugate, can slowly penetrate through the membrane barrier to reach the peroxidase. Immunohistochemical experiments using the Fab' conjugate as a probe revealed that most thyroid peroxidase in the thyroid gland is located in the endoplasmic reticulum and perinuclear cisternae of the follicular cell, although a small amount could occasionally be detected in the apical membrane including microvilli. In contrast to previous reports, no thyroid peroxidase could be found in other cellular structures such as Golgi apparatus and apical vesicles by the immunohistochemical technique employed.

Animals↗
PubMed1984

Increase in autorosette-forming lymphocytes in thyroid diseases.

A subpopulation of lymphocytes forming rosettes with autologous erythrocytes was studied on peripheral blood and thyroid tissues obtained from the patients with various thyroid diseases. The mean (+/-S.D.) percentage of autorosette-forming cells (ARFC) was 10.1(+/-5.5)% in the peripheral blood from patients with hyperthyroid Graves' disease, which was higher than that in normal subjects (5.6 +/- 2.8%), while the levels of ARFC in the peripheral blood from euthyroid patients with Graves' disease under treatment and Hashimoto's thyroiditis did not significantly differ from the normal level. The mean percentages of ARFC in the thyroid tissues from patients with Graves' disease and Hashimoto's thyroiditis were 14.7(+/-8.5) and 13.3(+/-7.8)%, respectively, which were higher than those in the peripheral blood from the same patients. Most of these cases with abnormally high levels of ARFC were accompanied with the abnormally low T cell to B cell ratios. The microscopic examination of the cytological materials from these patients showed an increased number of large stimulated lymphoid cells or lymphoblasts as compared with those who had few ARFC. These results suggest an increase in an activated T cell subset in the circulation and/or in the thyroid tissue, which is probably caused by active immune response to some stimuli.

Adolescent↗
PubMed1983

Bizarre leiomyoblastoma (epithelioid leiomyosarcoma) of the uterus--a case with a malignant clinical course.

A case of a bizarre leiomyoblastoma (epithelioid leiomyosarcoma) which developed in the uterus of a 64-year-old woman and showed a malignant clinical course was reported. Histologically, the tumor in the present case had a number of bizarre giant cells, while the portion showing an epithelioid pattern was minor. Since the existence of an intimate inter-relationship between tumor cells and the smooth muscles of the blood vessel wall was a characteristic finding in the present case, a possible role of vascular smooth muscles as a tumorigenic matrix was suggested. Because of the closure of the internal os of the uterus by the tumor, the uterus had a cystomorphous appearance, and contained about 800ml of intrauterine fluid. The highest number of mitosis observed was 7 per 10 HPF. In view of abundant existence of intranuclear cytoplasmic inclusions, the malignancy of the present case was strongly suspected from a histological diagnostic point of view. The patient died 5 months after surgery because of metastases to the lumbar vertebra and to the lung.

Female↗
PubMed1982

Developmental stages of Dirofilaria immitis in the dog.

Thirty-six Beagles were inoculated with 3rd-stage infective Dirofilaria immitis larvae to determine when 3rd and 4th molts occurred, how long each stage of development persisted in muscle and skin, and the patterns of larval migration from the infection site to the heart. From 22% to 84% of these larvae were recovered when the dogs were euthanatized and necropsied (mean recovery 45%). Larvae were recovered only from the skin and muscle during the first 58 days. The 5th-stage immature adults were first recovered from the heart in dogs killed on postinoculation day (PID) 70. Migration to the heart was essentially completed in dogs killed on PID 120, although 1 immature adult was recovered from subcutaneous tissues of each dog killed on PID 140 and 142. Starting and completion days of molting periods were recorded along with body lengths of male and female worms from the 3rd, 4th, and 5th stages. Microfilariae were first recovered from the peripheral blood in dogs killed on PID 190. Optimal time for drug evaluation against a particular stage of larval development is as follows: 3rd-stage larvae, PID 0 to 2; 4th-stage larvae, PID 15 to 50; and 5th-stage or immature adults, PID 65 to 120.

Animals↗
PubMed1981

Spontaneous autoimmune thyroiditis in the rat accelerated by thymectomy and low doses of irradiation: mechanisms implicated in the pathogenesis.

Factors involved in the production of autoimmune thyroiditis in thymectomized and sublethally irradiated rats were investigated. The study suggested that a gene linked to RT1 of the rat major histocompatibility complex (MHC), a selective depletion of suppressor T cells and high radiosensitivity of the thyroid gland were required in varying degrees to initiate the autoimmune thyroiditis in these rats. K cells in the spleen markedly increased at the initial stage and subsequently decreased at the appearance of the thyroid lesion, suggesting the consumption of K cells by thyroid antigen--antibody complexes formed in situ and in the circulation. Our data generally support the three genes concept proposed by Rose et al. (1976, 1980) that at least three genetically determined defects participate in triggering the production of autoimmune thyroiditis--namely, Ir genes within the MHC of the species, diminished ability of T cells to suppress autoimmune responses and a genetic defect in the thyroid gland.

Animals↗
PubMed1981

A case of ovarian endometrioid carcinoma and malignant transformation of adenomyosis uteri.

The perusal of literatures revealed that simultaneous malignant transformation of predisposing ovarian endometrioid tumor and uterine adenomyosis is a rare occurrence. A case report of endometrioid carcinoma in the right ovary and malignant transformation of adenomyosis in the uterus in a 52-year-old female was described. Both foci were histologically classified as mature adenoacanthoma and separately originated from the so-called endometriosis. There is no continuity associated with metastasis between the two carcinoma foci.

Adenocarcinoma↗