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Biomedical subjects

T Konno

Publications and source records attributed to T Konno.

At least 361 records · Page 20Linked to original sources
PubMed1977

Essential fatty acid deficiency induced by total parenteral nutrition and by medium-chain triglyceride feeding.

In hospitalized infants receiving either prolonged total parenteral nutrition without fat or a formula of medium-chain triglyceride, the fatty acid composition of platelet, red blood cell, and plasma lipids was determined. The results showed that the changes in the fatty acid composition occurred not only in plasma but also in platelets and red blood cells, and the decrease in linoleic and arachidonic acid and the concurrent increase in 5,8,11-eicosatrienoic acid were confirmed to be dramatic evidence of essential fatty acid deficiency. There was no effect of essential fatty acid deficiency upon the phospholipid distribution in red blood cells or plasma.

Animals↗
PubMed1977

Reovirus-like agent in acute epidemic gastroenteritis in Japanese infants: fecal shedding and serologic response.

The reovirus-like agent, sometimes referred to as duovirus or rotavirus, was visualized by electron microscopy in stool extracts from Japanese infants and young children with acute epidemic gastroenteritis. The virus particles measured 70 nm in diameter and had double-shelled capsids. One hundred ten (89%) of 124 patients with the gastroenteritis had such virus particles in stools obtained during the acute phase. The virus particles were excreted in the stools usually during the first eight days of illness. Agglutination of virus particles by antibody present in convalescent-phase sera was demonstrated by immune electron microscopy. Complement-fixing antibody was detected as early as day 3 of illness, and antibody titers peaked during the second and third weeks of the disease. The antibody appearing in the acute and early convalescent phases was sensitive to 2-mercaptoethanol. Antibody resistant to 2-mercaptoethanol was produced approximately 10 days after the onset of the symptoms. The serologic evidence suggests that a primary infection with the reovirus-like agent was responsible for the clinical attack of acute gastroenteritis.

Acute Disease↗
PubMed1977

The occurrence of electron dense intercellular materials and gap junctions in the human intestinal epithelium.

Electron microscopy of biopsy specimens from the small intestines in infants and children with various gastrointestinal disturbances revealed that the intercellular space of the mucosal epithelium was often filled with electron dense materials. Here apposed cell membranes were arranged almost parallel with each other. The dense materials also filled the space just subjacent to the epithelium to form a thick, dense layer in which the basal lamina was completely buried. The gap junctions were especially well developed in the epithelia filled with these materials. Long-period fasting of patients seemed to facilitate the occurrence of the dense materials and the formation of gap junctions.

Adolescent↗
PubMed1977

Antibacterial activity detected in duodenal juice.

Antibacterial activity could be detected in bacterium-negative duodenal juice from 3 patients, whereas it was not detected in bacterium-positive duodenal juice from other 3 patients. The follow-up study revealed that appearance of the activity in duodenal juice was just coincident with disappearance of bacteria in duodenal juice. The activity was not influenced by pH of the duodenal juice. These results seem to suggest that some antibacterial activity, different from that of pH, in duodenal juice regulates in some way proliferation of the upper gastrointestinal flora.

Adult↗
PubMed1976

A simple method for isolation of human properdin by affinity chromatography.

Human properdin was prepared from the zymosan eluate or euglobulin fraction of serum by immunoadsorbent column chromatography. An immunoadsorbent column containing antibody to human serum was employed to remove serum proteins other than properdin from zymosan eluates. Immunoadsorbent with antibody to properdin was used for direct isolation of properdin from the euglobulin fraction. Properdin isolated by these methods showed satisfactory purity with high activity. No immunochemical differences between the two types of preparation were found.

Animals↗