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Biomedical subjects

T Konno

Publications and source records attributed to T Konno.

At least 325 records · Page 18Linked to original sources
PubMed1981

A case with glucagonoma syndrome--endocrine and metabolic studies.

The results of clinical endocrine and metabolic studies on a 57-year-old female with surgically and autopsy verified glucagonoma syndrome were presented. All of the clinical manifestations of glucagonoma syndrome so far reported in the literature were noted but there was no evidence indicating the presence of multiple endocrine adenomatosis. The plasma IRG level was always more than 20 times above the normal, and the IRG response to insulin and tolbutamide injection was abnormal and the results of the other endocrinological studies revealed less remarkable features, if any. The surgically removed metastatic tumor of the liver contained an enormous amount of IRG and an appreciable amount of IRI, indicating that the elevated plasma IRG was mainly of tumor origin. These results clearly indicate that in glucagonoma there is some abnormality in glucagon release from the tumor. In addition to these findings, hypocalcemia, cardiac left ventricular hypertrophy and gastrointestinal dysfunction reportedly due to hyperglucagonemia were also seen in this patient.

Adenoma, Islet Cell↗
PubMed1981

Ultrastructural studies on congenital generalized fibromatosis regressed spontaneously.

Cellular components of a subcutaneous nodule from a male infant with congenital generalized fibromatosis consisted of myofibroblasts, fibroblasts, and their various intermediate types. The myofibroblasts were characterized by the presence of bundles of microfilaments and dense bodies similar to those in smooth muscle cells. Membrane-bound vesicles containing dense material were abundant in the extracellular space. They were probably lysosomes released by disintegration of cellular components. A possible role of the myofibroblasts and extracellular lysosomes in the regression of nodular lesions was discussed.

Fibroma↗
PubMed1981

Estimation of B-cells transformed by Epstein-Barr virus in patients with congenital agammaglobulinemia.

In vitro immunoglobulin synthesis was measured in lymphocytes from four patients with congenital agammaglobulinemia (cA gamma) stimulated by two different polyclonal B-cell activators, pokeweed mitogen (PWM) and Epstein-Barr virus (EBV). In PWM-stimulated cultures, patient T-cells treated with mitomycin C (MMC) were able to help the immunoglobulin (Ig) synthesis of normal B-cells. Patient B-cell-enriched fraction not containing surface Ig positive cells did not produce Ig in combination with MMC-treated autologous or allogeneic T-cells. Patient lymphocytes were infected with EBV and the subsequent production of Ig was measured. In lymphocytes from control subjects, exponential growth of the cells having EBV-associated nuclear antigen (EBNA) was shown to be associated with an exponential increase in Ig secretion within 1 week after EBV infection. However, in lymphocytes from three of the four patients, it took 2, 4 and 10 weeks, respectively, until lymphocyte-transformation and subsequent Ig-secretion were observed. Lymphocytes from one patient were not transformed nor did they secrete Ig after EBV infection. These results may imply that a small number of B-cells are present in peripheral blood of most of patients with cA gamma, and that they are able to produce the Ig after transformation by EBV which takes a much longer time than in controls.

Agammaglobulinemia↗
PubMed1981

Rotavirus infection in children with acute gastroenteritis in Ecuador.

Electron microscopic examination of stools of 702 infants and young children hospitalized in the Emergency Service of the Alejandro Mann Children's Hospital in Guayaquil, Ecuador, between August 1978 and October 1979 showed rotavirus to be present in 148 (21.1%). During the study period rotavirus was detected throughout the year, with no distinct seasonal variation. In addition to rotavirus, adenovirus was detected in stools of five (0.7%) of the patients and unidentified small round virus particles in seven (1.0%).

Acute Disease↗
PubMed1980

Establishment and characterization of a human acute monocytic leukemia cell line (THP-1).

A human leukemic cell line (THP-1) cultured from the blood of a boy with acute monocytic leukemia is described. This cell line had Fc and C3b receptors, but no surface or cytoplasmic immunoglobulins. HLA haplotypes of THP-1 were HLA-A2, -A9, -B5, -DRW1 and -DRW2. The monocytic nature of the cell line was characterized by: (1) the presence of alpha-naphthyl butyrate esterase activities which could be inhibited by NaF; (2) lysozyme production; (3) the phagocytosis of latex particles and sensitized sheep erythrocytes; and (4) the ability to restore T-lymphocyte response to Con A. The cells did not possess Epstein-Barr virus-associated nuclear antigen. These results indicate that THP-1 is a leukemia cell line with distinct monocytic markers. During culture, THP-1 maintained these monocytic characteristics for over 14 months.

Cell Line↗
PubMed1980

Demonstration of IgG EA (early antigen) and IgM MA (membrane antigen) antibodies in CMV infection of healthy infants and in those with liver disease.

The sequence of antibody production to various virus-specific antigens in CMV infection in infancy was studied. In healthy infants, IgG EA antibody was demonstrated in 18% of cord sera, and disappeared within two months after birth in all cases not shedding virus. The nonmaternal EA antibody was produced following virus excretion and decreased rapidly following cessation of virus excretion. Thus, demonstration of EA antibody in infants after 2 months of age was found to indicate acquired CMV infection, even when CMV could not be isolated. IgM MA antibody did not persist as long as EA antibody, disappearing before cessation of virus excretion. Both IgG EA and IgM MA antibodies were more frequently demonstrated in infants with hepatitis than in healthy infants. These findings suggest the possible association of CMV with hepatitis in infants.

Antibodies, Viral↗
PubMed1980

Analysis of immunological responses in psoriatic lesions: (1) immunopathological studies on psoriatic lesions.

In order to understand immunological responses in psoriatic lesions, immunofluorescent findings have been compared in early psoriatic lesions and fully developed plaque lesions. IgG and IgA were detected as the main antibodies in the horny layer by immunohistology. The IgA at the site of leukocyte accumulation in the horny layer seemed to be associated with secretory component (S-component) and joining chian. Regarding complement, the alternate pathway might be related to the immune reaction as the initial response, because deposits of C3, properdin and glycine rich beta-glycoprotein were found more frequently than C1q-deposit in the both lesions. In the dermis S-component was also detected at the dermal papillae adjacent to the dermoepidermal (D-E) junction of the lesions and no deposit of S-component was observed in uninvolved skin of psoriatic patients. This, however, is not a specific pattern in psoriatic lesions since deposits of S-component were found at the dermal papillae and D-E junction in other dermatoses. These findings might suggest that the immunological response was initiated by secretory IgA in horny layer, which was composed of dimeric IgA and S-component at the D-E junction and which passed easily through the epidermal cells.

Adolescent↗
PubMed1980

Epstein-Barr virus-induced lymphoblastoid cell lines from patients with primary immunodeficiency diseases.

Peripheral lymphocytes from eight patients with congenital immunodeficiency diseases were infected with Epstein-Barr virus (EBV) in an attempt to establish B lymphoblastoid cell lines (LCL). These patients included three boys with congenital agammaglobulinaemia, two girls with hypogammaglobulinaemia, one boy with common variable immunodeficiency, one boy with severe combined immunodeficiency with adenosine deaminase deficiency, and one boy with DiGeorge syndrome. Five of the patients bore no surface immunoglobulins (sIg) on their peripheral lymphocytes. LCL were established from seven of the eight patients. All the LCL established formed rosettes with EAC3 and had the ability to produce cytoplasmic immunoglobulins (cIg) of various classes. Culture supernatants concentrated up to 100-fold developed precipitin bands by Ouchterlony's method with antisera to human Ig in all the established LCL. These results suggested that both sIg-, cIg- and C3+ cells and sIg+, cIg- and C3+ cells might be the target cells for EBV and that sIg-, cIg- and C3+ cells might be the precursor cells of B lymphocytes.

Adolescent↗
PubMed1980

Age-dependent change in intestinal absorption of dipeptides and their constituent amino acids in the guinea pig.

The influxes from the mucosal solution to the enterocytes were investigated for glycylglycine, glycyl-L-leucine, and their constituent amino acids in isolated intestinal segments from suckling (3 to 4 days), weanling (10 to 14 days), and adult guinea pigs. Glycine and L-leucine influxes from the mucosal solution containing free glycine or L-leucine were not significantly different between sucklings and adults in either the jejunum or the ileum. On the other hand, glycylglycine and glycyl-L-leucine influxes were significantly greater in the following sequence: sucklings > weanlings > adults. Such age-dependent difference in dipeptide influx was more prominent in the jejunum than in the ileum. Kinetically, such developmental change in dipeptide influx was found to be related to the change in the value of Jmax (the maximum influx). The data indicated that the intact-peptide transfer mechanism has more important significance in protein hydrolysate absorption in immature stages of animals than in adults.

Aging↗
PubMed1979

Various types of corticotectal neurons of cats as demonstrated by means of retrograde axonal transport of horseradish peroxidase.

The retrograde labeling of cortical neurons with horseradish peroxidase (HRP) was used to investigate the morphological features of neurons in various cortical areas projecting to the superior colliculus in the cat. Corticotectal cells were found to be labeled in layer V of the entire cerebral cortex. The number of labeled cells and their locations varied according to the sites of injections of HRP in the colliculus. Most of the corticotectal cells identified in the present study were small (9--20 micrometer in diameter, 66%) and medium (20--40 micrometer, 30%) pyramidal neurons and only 4% of them were large (more than 40 micrometer). The labeled cells, 261 in total number, had somal diameters of 20.8 +/- 8.0 micrometer (mean and SD). The range of sizes of the labeled neurons was different in different cortical areas. For example, the labeled neurons in the Clare-Bishop area had a greater proportion of large diameter cells than in other areas. The present findings are largely in agreement with the previous data of anterograde degeneration methods with respect to the topographical correlation of the corticotectal projections. However, in some cortical areas, e.g., the sensorimotor and the first visual (area 17) cortex of the lateral surface of the hemisphere, relatively small numbers of corticotectal neurons appear to have been labeled by retrogradely transported HRP. The sparsity of the labeled neurons in certain cortical areas may reflect the existence of corticotectal neurons with axon collaterals supplying brain structures other than the superior colliculus.

Animals↗
PubMed1979

The occurrence of calicivirus in infants with acute gastroenteritis.

Calicivirus was detected in 8 (1.2%) of 647 hospitalized patients during a survey of acute gastroenteritis in infants and young children, conducted between December 1974 and September 1977. Morphologically calicivirus was approximately 30 nm in diameter with an easily recognizable staining "star of David" configuration. Its buoyant density in cesium chloride was 1.38-1.40 gm/ml. The serologic response to calicivirus by immune electron microscopy (IEM) was demonstrated only in paired sera from patients who shed the virus in their stools. The results suggest that calicivirus might be a cause of acute gastroenteritis in infants and young children.

Acute Disease↗