Search PubMed⌕ Search

Biomedical subjects

T Konno

Publications and source records attributed to T Konno.

At least 289 records · Page 16Linked to original sources
PubMed1983

Two non-T/non-B leukemia cell lines (THP-3-1 and THP-3-2) established from a patient at different stages of the disease.

Two leukemia cell lines of different phenotypic expression were established from a single patient with non-T/non-B acute lymphocytic leukemia (ALL); one (THP-3-1) was derived from the peripheral blood taken on admission before chemotherapy and the other THP-3-2) during the terminal stage. Both THP-3-1 and THP-3-2 cells were positive for terminal deoxynucleotidyl transferase (TdT) and Ia-like antigen but negative for surface immunoglobulins (Ig), nuclear antigen of Epstein-Barr virus (EBNA) and receptors for sheep erythrocytes. However, there was a difference in the expression of common ALL antigen (J5) between the two cell lines. J5+ cells were found in only 5% of THP-3-1 and in 96% of THP-3-2.

Antineoplastic Agents↗
PubMed1983

Ecto-5'-nucleotidase activities in B lymphoblastoid cell lines at various stages of maturation.

B lymphoblastoid cell lines (LCL) at various stages of maturation, including possible precursor B cell lines without immunoglobulin (Ig) expression, pre-B cell-like LCL, and Ig-secreting LCL, were established from peripheral and bone marrow mononuclear cells of four patients with congenital agammaglobulinemia (CAG) by infection with Epstein-Barr virus (EBV) and estimated for their ecto-5'-nucleotidase (ecto-5'-NT, E.C. 3.1.3.5) activity. Ecto-5'-NT activities in Ig-secreting LCL derived from CAG patients fell into the same range of those in LCL derived from control subjects (from 7.3 to 33.0 nmoles/hr/10(6) cells). When ecto-5'-NT activities of precursor B cell lines derived from the CAG patients were also compared with Ig-secreting LCL derived from control subjects, no significant difference in ecto-5'-NT activities was found among LCL with various Ig expression which might indicate various stages of B cell maturation. These results may suggest that ecto-5'-NT does not appear to be a biochemical marker of B cell maturation.

5'-Nucleotidase↗
PubMed1983

[Intra-arterial injection of an oily antineoplastic agent in hepatic cancer].

A lymphographic agent, Ethiodol, injected via the hepatic artery was found to remain selectively in the tumor vessels of hepatoma for a long time in our clinic. Taking advantage of this selective continuous peripheral embolization, a lipophilic high molecular anticancer agent, SMANCS (Copolymer of styrene maleic acid conjugated to Neocarzinostatin) dissolved in Ethiodol was administered via the celiac axis or the hepatic artery with Seldinger's method. Anticancer effect was examined by histological findings of specimens removed using hepatic resection (13 cases) and autopsy (1 case) in 14 patients receiving this treatment. Anticancer effect of this treatment became clear through histological findings. In the patients administered SMANCS more than 0.26 mg per 1 cm2 of maximum cut-surface area, complete or widespread necrosis of the tumor occurred, whereas non-cancerous liver tissue remained unaffected.

Adult↗
PubMed1983

[Oyster-associated hepatitis A].

We have experienced ten cases of hepatitis, type A, from March to April in 1983. All patients have taken raw oyster before hepatitis developed. The symptoms such as fever and/or diarrhea developed within 4 weeks after the patients have taken oyster. No cases of hepatitis in children were found out. From these results, it was concluded that the causes of hepatitis, type A, which we have experienced, are associated with raw oyster. HAV was not detected in shellfish by the method of radioimmunoassay.

Adult↗
PubMed1983

Primary yolk sac tumor of the liver. An autopsy case with ultrastructural and immunopathological studies.

An autopsy case of yolk sac tumor of the liver in a 29-year-old female was reported. The tumor was found originally in the right anterior segment of the liver on the first operation. The autopsy disclosed that the liver was almost entirely replaced by multiple, round tumor nodules. The tumor masses continuously extended to the peritoneum and to the subcutaneous tissue. There were no tumors in other organs including the ovaries, gut, stomach, lung, uterus, and kidneys, indicating that the tumor originated in the liver. Microscopically, the tumor showed typical characteristics of the yolk sac tumor. An immunohistochemical study showed that AFP was distributed fine granularly in the cytoplasm of tumor cells. Electron microscopic observation disclosed large amounts of electron-dense material being closely related to basement membrane.

Adult↗
PubMed1983

[Experimental study on maintenance of energy metabolism of the preserved liver].

The present studies were designed to achieve a good maintenance of energy metabolism as a means of improving the quality of liver preservation in rats. For this purpose, investigation were made whether initial warm ischemia may affect energy metabolism during preservation and oxygen supply during initial cold flushing process may protect energy metabolism. The following are the results. Compared with non-oxygenated groups, the oxygenated groups during the initial cooling process proved to be effective in, 1) reducing the precipitous fall in the ATP level of the liver during the initial cooling process, 2) maintaining the higher ATP level for 8 hours of hypothermic immersion storage, 3) maintaining energy production for 24 hours in intermittent perfusion storage, 4) obtaining a more rapid recovery of energy production in rewarming and reperfusion after 8 or 16 hours of hypothermic immersion storage, 5) obtaining the higher ATP level by high flow rate of flushing. It may be concluded that maintenance of energy metabolism in the preserved liver is related to the degree of interference with energy metabolism during the initial cold flushing and this interference can be prevented by flushing with the oxygenated perfusate of high flow rate.

Adenosine Triphosphate↗
PubMed1983

[Experiences with blood component transfusion and plasmapheresis during the last 6 months].

Our experiences with blood component transfusion and plasma exchange therapy during the last 6 months were reported. Total amounts used were 1,100 units with thrombocytes and 15 times (1 X 10(10) cells each time) with granulocytes obtained by centrifugation. Plasmapheresis was done 11 times with 7 patients and results obtained were compared with those of filtration leucapheresis which was done 100 times. Advantages and disadvantages of these methods are discussed.

Adolescent↗
PubMed1982

Astrovirus-associated epidemic gastroenteritis in Japan.

An outbreak of epidemic gastroenteritis associated with astrovirus, the first case reported in Japan, is described. Not only children (5-6 years of age), but also staff members of a kindergarten were affected. the virus particles detected in stools were 28-30 nm in diameter with a circular outline and had the characteristic star-like configuration which allowed identification as astrovirus. Significant immune responses to the virus were confirmed by immune electron microscopy. Out of 84 children, 43 (54.2%) were affected; the common symptoms were vomiting, abdominal pain, diarrhea, and fever. All the patients recovered completely within 48 hours. Occurrence of gastroenteritis due to contact with these patients was observed in 14 of 43 families.

Antibodies, Viral↗
PubMed1982

D-lactic acidosis in two patients with short bowel syndrome: bacteriological analyses of the fecal flora.

Two cases of D-lactic acidosis associated with short bowel syndrome are described. The administration of kanamycin to the patients showed a decrease in D-lactate in blood and urine in parallel with disappearance of metabolic acidosis. Bacteriological analyses of the fecal flora showed an increase in Lactobacillus buchneri in the first patient and Lactobacillus fermenti IVa in the second; both bacteria were sensitive to kanamycin. Quantification of in vitro production of D-lactate by each species of bacteria isolated from the feces revealed that Lactobacillus produced more D-lactate than other species of bacteria. These observations indicate that Lactobacillus may play an important role in the induction of D-lactic acidosis in patients with short bowel syndrome.

Acidosis↗