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Biomedical subjects

T Kohama

Publications and source records attributed to T Kohama.

89 records · Page 5Linked to original sources
PubMed1985

Noninvasive screening procedure for evaluation of bladder emptying function.

Bladder emptying function has been initially evaluated by uroflowmetry and post void residual urine measurement. Post void residual urine volume was commonly measured by a transurethral catheter which was not desirable to some patients. In the present study 7 patents with a complaint of dysuria were subjected to combined uroflowmetry and detection of post void residual urine using sector transabdominal ultrasound scanning. The volume estimation with assumption of bladder as an ellipsoid was not so accurate, nevertheless detection of post void residual urine itself was possible in all cases. Thus, the combined method appears to be very useful for evaluating bladder emptying function.

Aged↗
PubMed1984

Biological functions of monospecific antibodies to envelope glycoproteins of Newcastle disease virus.

Monospecific antisera to HN and F glycoproteins of Newcastle disease virus were prepared, and their effects on the biological activities of the virus were investigated. Anti-HN serum inhibited hemagglutinating and neuraminidase activity, as well as hemolysis. Anti-F serum had no effect on hemagglutination or neuraminidase but inhibited hemolysis and virus-induced cell fusion. Anti-HN serum was highly neutralizing, while neutralization by anti-F serum was very inefficient in conventional plaque reduction tests, although both sera were estimated to contain comparable amounts of antibody reacting with the virus as indicated by complement fixation and immuno-diffusion tests. The neutralizing activity of anti-F serum was greatly enhanced by the addition of anti-IgG serum or fresh guinea pig serum, whereas that of anti-HN serum was little enhanced. Anti-HN serum incorporated in the agar overlay suppressed the development of plaques to some degree, while anti-F serum had little effect. The combination of anti-HN and anti-F sera resulted in a marked decrease in the number and size of plaques, demonstrating the synergistic effect of the two species of antibody in the containment of the spread of viral infection.

Animals↗
PubMed1982

Cell-free coupling of Newcastle disease virus RNA transcription, translation and Co-translational processing.

A cell-free coupled system for transcription, translation and glycoprotein processing of the Newcastle disease virus genome is described. The system consists of a rabbit reticulocyte lysate preincubated with micrococcal nuclease and of detergent-disrupted purified Newcastle disease virions. [35S]methionine incorporation was linear for 2 h. Polypeptides NP and M, the presumably unglycosylated analogues of glycoproteins HN and possibly F, were identified as translation products. When in vitro synthesis was carried out in the presence of dog pancreas microsomes the HN analogue (pre-HN) was converted to an 80K (approx.) protein which comigrated on polyacrylamide gels with HN synthesized in vivo and which, except for a small fragment, was protected from proteolytic degradation. In immunoprecipitation studies, antiserum against HN purified from virions reacted with both the processed and the unprocessed form of HN synthesized in vitro.

Animals↗
PubMed1980

Proteolytic activation of the haemagglutinin-neuraminidase of Newcastle disease virus involves loss of a glycopeptide.

The uncleaved (HN0) and the cleaved (HN) forms of the haemagglutinin-neuraminidase glycoprotein of Newcastle disease virus (NDV), strain Ulster, were analysed by polyacrylamide gel electrophoresis under reducing and non-reducing conditions. When HN0 is converted into HN, a glycopeptide with an apparent mol. wt. of about 8000, which is not found in the mature spike, is removed.

Glycopeptides↗