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Biomedical subjects

T Kobayasi

Publications and source records attributed to T Kobayasi.

At least 91 records · Page 5Linked to original sources
PubMed1977

Electron microscopic cytochemistry for demonstration of sodium fluoride sensitive adenyl cyclase in normal human epidermis.

Adenyl cyclase activity in normal human epidermis was detected by an electron microscopic cytochemical technique. The sodium fluoride sensitive receptors were demonstrated on the outer sheath of the cell membranes. Adenyl cyclase activity was demonstrated in the basal cells and in the 4-5 lower layers of the Malpighian cells, while the superficial layers, stratum granulosum and stratum corneum showed no activity. The findings confirm and extend previous biochemical studies and provide a clue to the conception of the adenyl cyclase enzyme as a transmembrane arranged system.

Adenylyl Cyclases↗
PubMed1977

Pachyonychia congenita Jadassohn-Lewandowsky: a disorder of keratinization.

A 15-month-old boy with pachyonychia congenita is described. The patient also had follicular keratosis, leukokeratosis of the tongue, and blisters on the soles. Histopathological examination of the follicular keratosis showed hyperkeratosis and acanthosis. Horny plugs were located in sweat pores. By electron microscopy abnormal keratinization was demonstrated.

Darier Disease↗
PubMed1976

Lysolecithin fusion of cells from multiple sclerosis patients with Vero cells.

Investigations were performed on cell cultures derived from patients with multiple sclerosis (MS) in order to trace a possible virus infection as a cause of the disease. Cell cultures were established from one brain autopsy specimen and four lymph node biopsies from MS patients. Lymphocytes from 28 MS patients and six healthy controls were used for fusion or cocultivation experiments, either immediately after isolation or after mixed lymphocyte cultures (MLC). Lysolecithin fusion and cocultivation experiments were made with Vero cells and with, respectively: cultured brain cells, lymph node cells and lymphocytes from MS patients. Electron microscopical examination revealed intranuclear filamentous structures in 5 per cent of the cells in primary cultures of MS brain and lymph node and in control skin organ cultures. Multinucleated cells were found in six out of 19 cocultures of Vero cells and MS lymphocytes preincubated for 2 days at 37 degrees C. The cultures were tested for the presence of viruses, i.e. measles virus and virus producing hemadsorption with human type O and/or guinea pig erythrocytes and virus against which the MS patients showed serum FA-antibodies. No virus antigen could be demonstrated in the cells.

Adult↗
PubMed1976

Dissociation of human adult epidermal cells by disulfide-reducing agents and subsequent trypsinization.

Primary cultures of epidermal cells from human adult skin were dissociated by dithiothreitol (DTT) with subsequent trypsinization. After treatment with disulfide reducing agents, the desmosomes were either destroyed or showed a homogeneous ultrastructure. Trypsin separated the homogeneous desmosomes, whereas cell membranes, organelles, and nuclei remained undamaged. Trypsin did not influence normal desmosomes. A suspension of human epidermal cells was recultivated in new flasks. Two types of cells grew in the cultures--polygonal and spindle-shaped. The polygonal cells were maintained for 30 to 60 days and grew slowly. The spindle-shaped cells were leucine-amino-peptidase negative, grew actively, and were subcultured.

Adult↗
PubMed1976

Ultrastructure of localized myxedema.

Five biopsies from three patients with localized myxedema were studied by electron microscopy. The dermis showed an overwhelming accumulation of microfibrils with knobs (acid glycosaminoglycans) and an amorphous material containing glycoprotein. Furthermore, evidence of degradation without new formation of collagen fibrils was found. Considerable numbers of mast cells with various types of granules, fibroblasts with dilated granular endoplasmic reticulum, and macrophages were seen in the mucinous areas.

Adult↗
PubMed1975

Separation of human epidermal cells from fibroblasts in primary skin culture.

To obtain pure culture of epidermal cells from small human biopsies, two different techniques were tested and compared, i.e. separation of epidermis from corium before cultivation by trypsin and suction, and after cultivation by trypsin and collagenase. The most active growth of epidermal cells was obtained by the third technique, since short-term trypsin treatment released only fibroblasts from the culture. Crude collagenase (type I) was less effective than trypsin. Collagenase type II, III and IV had no effect on fibroblast release. Neither trypsin nor collagenases dispersed epidermal cells.

Biopsy↗
PubMed1975

Suction blister transplantation for leg ulcers.

Application of suction blister transplants to leg ulcers is an alternative symptomatic treatment to split-skin grafting, i.e. "pinch-grafting". Blisters are produced by the suction device Dermovac at a suction pressure of 250-300 mmHg for 1-2 hours. The blister-roofs are cut off at the periphery and placed on the granulation tissue of the ulcer. The healing time for leg ulcers was 10-14 days. Excellent epithelialization was seen in 10 out of 12 ulcers. The advantages of the method are, that it is easy to perform, no scarring is produced, and the procedure is painless.

Blister↗
PubMed1975

On the diagnosis of Fabry's disease.

Fabry's disease is a recessive X-linked inborn error of metabolism due to deficiency of the lysosomal enzyme alpha-galactosidase. The large variety of symptoms may make the diagnosis difficult. A severely afflicted female patient is presented. For several years she had been treated under the diagnosis polyarteritis nodosa until the characteristic cutaneous lesions of Fabry's disease were recognized. Enzymatic studies and electronmicroscopic examinations confirmed the diagnosis. A symptomatic effect of corticosteroid treatment was proven. The grave prognosis, the recent attempts at enzyme substitution therapy and the possibility of preventing new cases by prenatal diagnosis should stimulate the efforts of the clinician to diagnose the disease.

Adult↗
PubMed1975

Ultrastructural changes in scleromyxedema.

Skin biopsy specimens from a 60-year-old patient with paraproteinemia and generalized changes of the skin typical of scleromyxedema were studied with the electron microscope. The dermis was dominated by collagen fibrils and accumulations of peculiar connective tissue cells, while elastic tissue was sparse and in some areas completely absent. Large, sharply demarcated areas of a filamentous material were occasionally observed. The collagen fibrils were often surrounded by thin filaments with a periodic segmentation and by many glycosaminoglycan (mucopolysaccharide) filaments. The elastic fibres contained large amounts of elastic fibrils and small amounts of a homogenous matrix. The cytoplasm of the above-mentioned cells was dominated by lysosomes in different stages of development, often occupying almost all the cytoplasmic area. The collagen fibrils were found in close relation to these cells, frequently inside invaginations of the cells. Furthermore, collagen fibrils were observed free in the cytoplasmic area and inside the lysosomes, indicating lysosomal degradation of collagen.

Cell Membrane↗