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Biomedical subjects

T Kiyosawa

Publications and source records attributed to T Kiyosawa.

24 records · Page 2Linked to original sources
PubMed1991

[The change in immunohistochemical localization of basic fibroblast growth factor (b-FGF) around the lens capsule after extracapsular extraction].

It has been reported that basic fibroblast growth factor (b-FGF) accelerates proliferation of mesenchymal cells and epithelial cells of cornea and crystalline lens in vitro. The immunohistochemical localization of b-FGF on guinea pig lens capsule after extracapsular extraction (ECE) of the lens was studied in order to investigate the role of b-FGF on posterior capsular opacification after ECE and neovascularization. On the 12th postoperative day, immunohistochemical staining of b-FGF was recognized along the posterior capsule on the cortical side, but not along the anterior capsule. On non-operated eyes, scanty immunoreactive materials were seen in cells at the germinative center of the equatorial region. More than two months after ECE, almost no immunoreactive staining could be recognized. In this study, immunoreactive staining of b-FGF was recognized along the posterior capsule on cortical side after operation. It has also been reported that b-FGF stimulates proliferation of the lens epithelial cells in vitro. Therefore, it is suggested that b-FGF plays an important role on posterior capsular opacification after cataract formation.

Animals↗
PubMed1988

Vessel anastomosis using a venous cuff and two sutures: an experimental study in rat femoral and epigastric vessels.

Using rat femoral and epigastric vessels, the efficacy of an autogenous venous cuff and reduction in the number of required sutures were examined. Cut vessels were anastomosed with two sutures using 11-0 nylon, and the anastomotic site was covered with a venous cuff. After two weeks, the patency of the anastomosed vessels was evaluated. Although results in large-caliber femoral veins were poor, the femoral arteries and epigastric vessels demonstrated high patency rates. In microscopic and scanning electron microscopic evaluations, gaps and depressions between sutures were observed, but they were smoothly covered by endothelium. These results suggest the usefulness of the venous cuff in microvascular surgery, especially in anastomosing fine-caliber vessels.

Anastomosis, Surgical↗
PubMedDate not supplied

Trimethadione tolerance test for the quantitative assessment of liver function in rats.

The quantitative estimation of the hepatic functional volume in rats was attempted using the serum dimethadione (DMO)/trimethadione (TMO) ratio in a single blood sampling after oral administration of TMO, which we call the TMO tolerance test, in order to develop a means of pre-operatively assessing hepatic resectability. Serum DMO/TMO ratios correlated well with the total amount of the hepatic microsomal TMO-N-demethylase activity (enzyme activity) and with the remnant liver weight after 37% and 68% partial hepatectomy. These ratios increased after partial hepatectomy in parallel with the changes in the enzyme activity and the remnant liver weight. The results suggest that the quantitative functional changes of the remnant liver after hepatectomy in both CCl4-treated and untreated rats can be estimated pre-operatively by the TMO tolerance test.

Animals↗
PubMedDate not supplied

Vascular endothelial growth factor in angiosarcoma.

Angiosarcoma(AS) is a malignant tumour of the endothelium of lymphatic or blood vessels. Vascular endothelial growth factor (VEGF), a specific mitogen of endothelial cells, is considered essential for the growth of many tumours including AS. We have recently treated several patients with AS; in two of them, using a newly developed enzyme-linked immunosorbent assay (ELISA) for VEGF, we sequentially measured serum VEGF concentrations. While serial serum VEGF levels reflected tumour burden, the various VEGF concentrations were within the normal range we reported previously. Furthermore, the VEGF concentrations were not remarkably elevated in AS tumour tissue compared with benign vascular lesions and hypervascular tumours.

Adult↗