Search PubMed⌕ Search

Biomedical subjects

T Kitamura

Publications and source records attributed to T Kitamura.

At least 847 records · Page 47Linked to original sources
PubMed1977

Electron microscopic features of the resting microglia in the rabbit hippocampus, identified by silver carbonate staining.

Vibratome sections of hippocampus of adult rabbits were stained by a modified Hortega's silver-carbonate method. Impregnated materials were examined by electron microscopy to decide fine-structural characteristics of the resting microglia. Comparing their characteristics with those of macrophages, we came to the following conclusions: (1) Impregnated resting microglia in the hippocampus of adult rabbits can be identified as cells having distinct fine structures. (2) Resting microglia are morphologically different from macrophages or their precursor cells, and, therefore, seem not to be hematogenous cells sojourning in the normal brain parenchyma.

Animals↗
PubMed1977

Pathology of lupus nephritis with special reference to the immunological bases of glomerular changes.

Glomerular changes, and cellular and tissue responses of antibody-forming organs in 37 cases (20 cases with nephrotic syndrome and 17 cases without the syndrome) of systemic lupus erythematosus (SLE) were described. The glomerular changes were classified into 3 types, i.e., proliferative glomerulitis, membranous transformation, and wire-loop lesion from the standpoint of glomerular functional structure and its alterations. As to the responses of antibody-forming organs, hypoplasia of myeloid tissue, atrophy of lymph follicles of spleen and lymph nodes, atrophy and decrease in numbers of lymphocytes of thymus, proliferation and infiltration of plasma cells and plasmocytoid basophilic mononuclears, and proliferation of basophilic reticulum cells at the cost of differentiated antibody-forming organ tissues of bone marrow, spleen, lymph nodes, etc. were noted. Immunological bases of glomerular changes were considered and discussed in the light of pathological findings of SLE presented here and suggestions from experimental pathological studies in this field. Pathogenic roles of antigen-antibody complexes as well as anti-glomerular basement membrane (anti-GBM) antibody were considered in relation to the histologic manifestations of glomeruli in SLE or lupus nephritis.

Adolescent↗
PubMed1977

Demonstration of vaccinia virus antigen in brains of postvaccinal encephalitis cases.

Five lethal cases of postovaccinal encephalitis (PVE) were examined in attempts to detect the viral antigen by the fluorescent antibody techniques and to isolate the infectious vaccinia virus. Histologically, the brain tissues were characterized by meningoencephalitis, with perivascular infiltrations and inflammatous reactions mainly in leptomininx, chroid plexus and ependyma. By direct immunofluorescence staining, vaccinia virus antigen was found in the brain specimens of one case, but not in the other four cases. The existence of antigen-antibody complexes was suggested by the deposition of immunoglobulins in the brain specimens. After treatment with 3 M NaSCN solution to dissociate the immunoglobulins from the complex, the viral antigen was demonstrated in specimens of two cases. Localization of viral antigen(s) thus detected coincided with that of meningoencephalitic reactions in the histological examination. Virus isolation on chick embryo chorioallantoic membrane was negative in all cases. Participation of viral pathogenicity in the etiology of PVE is discussed.

Antigen-Antibody Complex↗
PubMed1977

Pathogenicity of a vaccine strain of vaccina virus in rabbits administered by intravenous route.

The pathogenicity of the Lister (Elstree) strain, a vaccinia virus, in rabbits after intravenous injection was studied by histopathological and immunofluorescent methods. Inoculation of 1 X 10(7) PFU (pock-forming unit) virus into weanling and young adult rabbits caused severe emaciation and high mortality within 2 weeks. Pathological findings were characterized by vesicular lesions along muco-cutaneous junction areas of the eyes, nose and mouth and by inflammatory changes in the brain, mainly in the meninges and choroid plexus. Immunofluorescent staining of the tissues of animals sacrificed at intervals demonstrated the accumulation of vaccinia viral antigen (s) in the loci of pathological changes. The suitability of this model system for the study of pathogenesis of human postvaccinal meningoencephalitis is discussed.

Animals↗
PubMed1977

A study on the nature of macroamylase complex. Dissociation of macroamylase by substrates.

Characteristics of a macroamylase that was considered not to be formed by the binding of normal amylase to immunoglobulins were studied. The macroamylase was reversibly dissociated into apparently normal amylase by treating the macroamylasemic serum with guanidine hydrochloride. Concanavalin A precipitated a large portion of the macroamylase. When the macroamylase was purified by affinity chromatography using an insoluble starch polymer at neutral pH, a considerable dissociation of the enzyme into apparently normal amylase was observed with the purified preparation. Such dissociation of the macroamylase was demonstrated by briefly incubating the patient's serum with a low-molecular weight starch, a substrate of the enzyme. This dissociated enzyme showed upon electrophoresis essentially the same isozymic pattern as that of normal serum amylase. When the patient's serum was incubated with the low molecular weight starch for a prolonged period to hydrolyze the starch completely and was dialyzed, reconstitution of macroamylase resulted. The amylase-binding substance(s) that is supposed to be involved in the formation of macroamylase was found to release when the enzyme was adsorbed on the insoluble starch polymer at 0 degrees C. The present results strongly suggest that the amylase-binding substance(s), probably polysaccharide(s) or glycoprotein(s), binds to the substrate-binding site of normal amylase to form a macroamylase complex.

Adsorption↗