Search PubMed⌕ Search

Biomedical subjects

T Kitamori

Publications and source records attributed to T Kitamori.

36 records · Page 2Linked to original sources
PubMed1999

Antiaggregatory, antithrombotic effects of MS-180, a novel platelet glycoprotein IIb/IIIa receptor antagonist.

The antiaggregatory and antithrombotic effects of (S)-(-)-ethyl[6-[4-(morpholinoformimidoyl)benzamido]-3,4-dihydro-2 H-1-benzo-pyran-3-yl]acetate hydrochloride (MS-180), a novel platelet glycoprotein IIb/IIIa receptor antagonist, were investigated. Ma-HCl, (S)-(-)-[6-[4-(Morpholinoformimidoyl)benzamido]-3,4-dihydro-2H-1-b enzopyran-3-yl]acetic acid hydrochloride, the hydrochloride salt of Ma (active metabolite), inhibited the binding of fibrinogen to immobilized human glycoprotein IIb/III receptor with an IC50 value of 0.12+/-0.03 nM without affecting binding to either fibronectin or vitronectin receptors. In anesthetized guinea pigs, intraduodenal administration of MS-180 caused dose-dependent inhibition of both ADP- and collagen-induced ex vivo platelet aggregation. At the same dosages, occluded thrombus formation and platelet release reactions were also markedly suppressed. In anesthetized dogs, the bleeding time was prolonged slightly even when submaximal inhibition (< 90%) of ex vivo platelet aggregation was achieved following i.v. administration of Ma-HCl. Aspirin (100 mg/kg) prolonged the bleeding time to the same extent as MS-180 (1 mg/kg), although it suppressed only collagen-induced platelet aggregation. Therefore, MS-180 may be clinically useful for the treatment of thrombotic diseases.

Acetates↗
PubMed1999

Systematic treatment for nocturnal urinary frequency following a sleep-micturition chart.

Seven patients complaining of nocturnal urinary frequency were treated following a sleep-micturition chart. By clinical analysis of the data, the causes of nocturnal urinary frequency were divided into three diagnostic categories: a small bladder capacity group, a sleep disorder group and a large nocturnal urinary volume group. The three groups were treated by anticholinergic agents, sleeping pills and restriction of water intake at night respectively. With the administration of anti-cholinergic agents or sleeping pills nocturnal bladder capacity increased. By restriction of water intake at night nocturnal urinary volume decreased. In all patients nocturnal urinary frequency decreased and sleep efficiency was improved.

Aged↗
PubMed1999

Effects of MS-31-038, a novel Na(+)-H+ exchange inhibitor, on the myocardial infarct size in rats after postischemic administration.

The abilities of 2-(2-methylphenyl)-5,7-dimethoxy-4-quinolyl carbonylguanidine dihydrochloride (CAS 181048-29-3, MS-31-050) and 2-phenyl-8-(2-methoxyethoxy)-4-quinolyl carbonylguanidine bismethanesulfonate (CAS 181048-36-2, MS-31-038) in inhibiting Na(+)-H+ exchange, ischemia- and reperfusion-induced injury were determined and compared with those of 4-isopropyl-3-methylsulfonylbenzoyl guanidine methanesulfonate (CAS 159138-81-5, IMGM), a selective inhibitor of Na(+)-H+ exchange. MS-31-050 and IMGM exhibited comparable inhibitory effects on Na(+)-dependent pH recovery and antiarrhythmic effects during ischemia in anesthetized rats. In rats subjected to ischemia and reperfusion, MS-31-050 (10 mg/kg i.v.) significantly reduced the infarct size when given prior to the onset of ischemia. However, postischemic treatment with either MS-31-050 or IMGM failed to protect reperfused hearts. In contrast, MS-31-038 reduced the infarct size dramatically from 65.4 +/- 7.4% in control to 29.9 +/- 11.6% at 3 mg/kg and 9.8 +/- 3.4% at 10 mg/kg even when administered before the onset of reperfusion. These results suggest the beneficial effects of Na(+)-H+ exchange inhibitors on myocardial ischemia/reperfusion injury.

Anesthesia↗
PubMed1997

In vivo determination of ultratrace amounts of prostaglandin in plasma by high-performance liquid chromatography/laser-induced fluorometry/ultrasensitive laser spectrometry under severe conditions.

We succeeded in determining ultratrace prostaglandin amounts in plasma, at the femtomolar level, using laser-induced fluorometry through a complete redesign of the analytical procedures. Practical samples, especially plasma, contain large amounts of admixtures, and prostaglandin in plasma (pg/mL) has been considered to be difficult to detect because the samples and reagents supplied by conventional procedures are neither pure nor stable enough to get good results by ultrasensitive laser spectrometry. We completely redesigned the analytical procedures after careful investigations of the reagent purification and the column separation conditions based on a newly found behavior of the reagent and derivatized prostaglandin in a small quantity of ethanol in the mobile phase. A lower determination limit of 23 pg/mL (65 fmol) was achieved, the variance was 12% at 25 pg/mL, and the recovery rate was 88-89%. This method was applied to in vivo analysis of the concentration of prostaglandin E1 administered as a prodrug of prostaglandin E1 (delta(8)-9-O-butyryl prostaglandin F1 butyl ester, AS-013) by intravenous infusion to beagle dogs. A clear correlation between the change of blood pressure and the prostaglandin E1 concentration was confirmed.

Alprostadil↗
PubMed1996

Ultrasensitive heterogeneous immunoassay using photothermal deflection spectroscopy. 2. Quantitation of ultratrace carcinoembryonic antigen in human sera.

We previously proposed a photothermal immunoassay based on highly sensitive detection by a photothermal beam deflection method using colloidal gold as a labeling material. The assay system was significantly improved in terms of background reduction of ultrasensitive laser spectrometry through the newly designed reaction and separation procedure using microspheres for a solid phase. Highly sensitive laser spectrometric analysis is often susceptible to interference by even a small amount of admixtures. In this subsequent study, we improved our photothermal immunoassay so that it possesses further selectivity, matching that of the highly sensitive photothermal detection method. It was proved that our method could be applied to determine ultratrace quantities of a carcinoembryonic antigen (CEA) in cancer patients' and normal sera. The lower detection limit of CEA was 0.078 ng/mL (9.8 amol, absolute quantity). This is 10 times superior to that of any other immunoassay method and has enough detectability to measure a low level of CEA in healthy individuals. Intraassay coefficient of variation is about 8% at eight different concentrations (n = 6). We assayed 61 serum samples, and there was a good correlation between this method and previously established ELISA. We obtained clearer demarcation of healthy persons and colon cancer patients in terms of the CEA value than was obtained by ELISA. We believe this is the first report in which the laser-based ultrasensitive immunoassay is proved to be applicable to human sera, although the matrix admixtures causing interference in the assay were quite different in each sample serum.

Carcinoembryonic Antigen↗
PubMed1996

[Long-term results of systematic treatment for nocturnal enuresis based on overnight simultaneous monitoring of electroencephalography and cystometry].

BACKGROUND: The long-term results of systematic treatment based on overnight simultaneous monitoring of electroencephalography (EEG) and cystometry in the patients with enuresis were evaluated. METHODS: From January to December a total of 213 patients were classified into 3 types. Enuresis Type I: such cases show a normal cystometrogram (CMG) with an awakening response on the EEG before enuresis, but they do not awake. Enuresis Type IIa; such cases show a normal CMG without an awakening response on the EEG. Enuresis Type IIb: such cases show an abnormal CMG with no awakening response on the EEG. When such cases enter the deep sleep stage, continuous uninhibited contractions of the bladder are observed on the CMG. 136 cases were of Type I, 20 cases of Type IIa, and 57 cases of Type IIb. RESULTS: Out of 213 patients who were followed up for 2 years, cured cases were 94 (44%), effective cases were 81 (38%) and unchanged cases were 38 (18%). In 136 patients with Type I, cured cases were 71 (52%), effective cases were 50 (37%) and unchanged cases were 15 (11%). In 20 patients with Type IIa, cured cases were 8 (40%), effective cases were 9 (45%) and unchanged cases were 3 (15%). In 57 patients with Type IIb, cured cases were 15 (26%), effective cases were 22 (39%) and unchanged cases were 20 (35%). The age of the effective group, which included cured cases and effective cases, was significantly higher than that of the unchanged group. In enuresis Type I, the percentage of the patients with incontinence in daytime were significantly in the unchanged group than in the effective group. No significant differences in the frequency of enuresis and the percentage of the patients who had awakened spontaneously by urinary sensation were found between the two groups. CONCLUSION: The therapeutic response was best in enuresis Type 1 and worst in enuresis Type IIb. The patients, in whom not only frequency of enuresis but also type of enuresis was unchanged by systematic treatment for two years, was approximately 10% across the types. Accordingly they were thought to be non-responders to this systematic treatment.

Child↗
PubMed1996

Urological abnormalities in 1,328 patients with nocturnal enuresis.

OBJECTIVES: Evaluation of the incidence of urological abnormalities as revealed by urological examinations of a large number of patients with enuresis. METHODS: Patients with the chief complaint of nocturnal enuresis were examined urologically. RESULTS: The incidence of urological abnormalities was 1.8% of 940 patients on intravenous pyelography (IVP), 7.1% of 695 patients on voiding cystourethrography (VCG) and 11.5% of 487 patients on cystometry (CM). No abnormal findings were observed in 58 patients on renal ultrasonography (US). 92.1% of reflux cases detected by VCG were low grade and only 8.9% of patients with reflux had pyuria. 20.2% of 446 patients who were submitted to all these examinations had some urological abnormality. Only pollakisuria was statistically more frequent in patients with urological abnormalities than in patients without them. CONCLUSION: These data suggest that the incidence of urological abnormalities was rather low when compared with the past literature. In particular, IVP was though to be unnecessary.

Child↗
PubMed1995

[Two cases of rhabdomyosarcoma of the bladder in children].

A 1-year-old boy was admitted to our hospital with chief complaint of urinary retention and a lower abdominal large mass. The mass was shown in the bladder by computerized tomography (CT) associated with paraaortic lymph node swelling. Tumor biopsy revealed rhabdomyosarcoma, embryonal type. Complete remission (CR) was obtained by chemotherapy based on STS' 88. He has been healthy 40 months without recurrence. A similar case of rhabdomyosarcoma, embryonal type, in the bladder in a 3-year-old girl with a chief complaint of macrohematuria was verified by CT and tumor biopsy. The same chemotherapy was performed, resulting in partial remission (PR). A complete resection of the tumor was achieved by partial cystectomy. She is alive without recurrence 18 months after the cystectomy.

Antineoplastic Combined Chemotherapy Protocols↗
PubMed1994

Treatment system for nocturnal enuresis according to an original classification system.

Our department has reported recent studies for nocturnal enuresis. The morning bladder capacity in enuretic children was smaller in the 3- and 4-year-old age range but larger in the 7-year and over age range than that in nonenuretic children. 15% of nonenuretic children showed nocturnal polyuria with nocturnal urination after complete awakening more than once per week. It was not likely from this evidence that an immature bladder capacity or nocturnal polyuria was the primary cause of enuresis. Patients with enuresis were classified into three types based on overnight simultaneous monitoring by electroencephalography and cystometry. The pathogenesis of enuresis type I and type IIa was a disturbance of awakening, while that of type IIb was a disturbance of bladder function not shown in the daytime but only at night. An original systematic therapeutic plan was established with the development of an original therapeutic machine primarily for enuresis type I.

Child↗
PubMed1993

Quantitation of IgE and carcinoembryonic antigen (CEA) by optical beam deflection (OBD) measurement of dot-immunobinding assay patterns visualized by an ELISA technique.

Dot-immunobinding assays of IgE and CEA were performed by a conventional dot-ELISA technique with diaminobenzidine staining, and the quantitative results were compared by densitometry and a new, spectroscopic, optical beam deflection (OBD) method using the same membrane. It was possible with the OBD method to detect quantities of these substances at least ten times smaller than with densitometry. Better intra-assay reproducibility for IgE and CEA measurements was obtained by the OBD method. The measurable ranges of the OBD method was broader than that of densitometry, because dark bands caused OBD in proportion to their color densities. When the dot-immunobinding assay with OBD measurement for CEA was also compared with a microtube ELISA using biotin-avidin conjugates, the sensitivities and reproducibilities of the two methods were found to be similar, with a correlation coefficient of 0.991.

Carcinoembryonic Antigen↗
PubMed1993

[Ofloxacin concentration in prostatic tissue].

The concentration of ofloxacin in prostatic tissue and serum was determined in order to evaluate the permeability of ofloxacin into prostatic tissue in patients with benign prostatic hypertrophy. Ofloxacin was administered orally at a dose of 200 mg several hours before subcapsular prostatectomy. The determination was performed in the surgically removed adenoma and in the serum taken one hour before and at the removal of the adenoma. The peak level in the prostatic tissue was 1.46 microgram/g at 4.5 hour after the administration. The ratio of prostatic tissue level to the serum level was 1.00. In conclusion, ofloxacin was thought to be very useful for the treatment of acute and chronic prostatitis.

Aged↗
PubMed1993

[Influential factors on the therapeutic response in the conditioning treatment of enuresis with an original therapeutic machine].

The conditioning treatment of enuresis with our original therapeutic machine, that is to awake the patient before enuresis may occur, has been performed since 1987. Influential factors on the therapeutic response were investigated. Twenty two patients with enuresis Type I were admitted and were treated for 5 nights with the therapeutic machine. Seven patients were cured (the cured group) and a certain effectiveness (decrease of the frequency of enuresis of more than 50%) was observed in 8 patients (the effective group). No effectiveness was obtained in 7 patients (the no change group). The average age of the cured group was higher than that of the no change group, and the difference was significant. No significant differences were found among the three groups in sex, the frequency of enuresis or the past experience of awakening before enuresis. Significant differences among the three groups were found in the average awakening score (how easily the patient awoke when a nurse called the patient after the machine alarmed) and the change of awakening score during treatment. The average awakening score of the cured group was the highest and that of the no change group was the lowest. The change of awakening score during treatment of the no change group was significantly lower than that of the cured group or that of the effective group. The desire to cure, scored 0-2 points at the time of discharge, was significantly stronger in the cured group than in the no change group. No significant differences were noticed among the three groups in the sleeping condition and the remembrance of awakening at the next morning.

Adolescent↗
PubMed1988

[Ofloxacin for the treatment of chlamydial urethritis].

The effect of Ofloxacin administered orally 600 mg everyday for 14 days was evaluated in 20 male patients with Chlamydial urethritis, in whom Chlamydia was identified from urethral secretion by immunohistochemical technique using monoclonal primary antibody specific for all known serotypes of Chlamydia trachomatis and psittaci. 19 out of the 20 patients showed an improvement of subjective and/or objective symptoms after the treatment. The Chlamydia reaction turned to negative in 13 patients and to (+/-) in another patient. The overall clinical effect was excellent in 7, good in 6 and poor in 7, showing a effective rate of 65%. No side effects were observed.

Adult↗
PubMed1987

A continuous sampling model of the seminal vesicle fluid in rat.

A continuous sampling model of the fluid excreted from the unilateral seminal vesicle was devised in the rat. Each 12 hr change in the volume was measured in 14 sexually mature male rats by use of tubing. If we assume the excretion of a small volume (less than 4 microliters) as the resting level, 3 of 14 rats showed only the resting level. The excretion of a large volume was sometimes observed in the 11 other rats both in daytime and nighttime. This model would be promising for investigations of the seminal vesicles from new aspects.

Animals↗