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Biomedical subjects

T Kimoto

Publications and source records attributed to T Kimoto.

At least 127 records · Page 7Linked to original sources
PubMed1983

Establishment and characterization of a human colon carcinoma cell line (KMS-4) from a patient with hereditary adenomatosis of the colon and rectum.

A human colon carcinoma cell line was established from a metastatic lymph node of a patient with hereditary adenomatosis of the colon and rectum (ACR). Cells of this line, designated KMS-4, have been continuously propagated in culture during the past 24 months. The cells growing on the surface of culture dishes showed epithelial features, and, when inoculated into athymic nude mice, produced adenocarcinomas with a morphology similar to that of the original tumor. Electron micrographs showed that the cultured cells have desmosomes and numerous surface microvilli typical of colon epithelium. Chromosomal analysis revealed the cell line to be of human origin with a diploid mode of chromosome number, but the karyotypes examined were all abnormal. Most of the metaphases commonly had such abnormalities as 7p+, 12p+, +13, +16 and 17p+, accompanied by loss of chromosome No. 19 and/or 20, and, interestingly, all the metaphases contained 7p+ and +13. The cells had a log phase doubling time of 48 to 72 h. The cloning efficiency of the cells was 0.06% in the soft agar medium. Neither 12-O-tetradecanoylphorbol-13-acetate nor bile acids enhanced cell proliferation. The cells abundantly secreted CEA protein into the culture medium (700 ng/ml/106 cells during a 48 h period). The present colon carcinoma cell line derived from a genetically defined individual with ACR should prove useful for research in human oncology or genetics.

Adenocarcinoma↗
PubMed1983

A lymphoblastoid cell line with high phagocytic activity established from peripheral blood of a patient with chronic myelocytic leukemia in blast crisis.

A human hematopoietic cell line (K-23-M) was established from a patient with chronic myelocytic leukemia in blast crisis. Morphologically, the cultured cells were lymphoblastoid cells that produced IgA and were Epstein-Barr viral nuclear antigen positive. But they showed high phagocytic activity to glutaraldehyde-treated sheep red cells and had properties of a monocyte or macrophage that included surface Fc receptors, alpha-naphthyl butyrate esterase positivity blocked by NaF, migration in soft agar and the ability to attach to a glass surface. Lysozyme secretion was absent, and chromosomes were diploid and Ph1 negative. This cell line is unique in that it has strong phagocytic activity. Its existence shows that lymphoblastoid cell line may be a more important cell line for the study of human hematopoietic cells than previously has been believed.

Antigens, Viral↗
PubMed1983

[Potentiation of the cytotoxic effects of various anticancer drugs by interferon on human neoplastic cells (HeLa) in culture].

Potentiation of the cytotoxic effects of various anticancer agents by interferon on human malignant cells was examined in culture. The human neoplastic cells used were HeLa cells derived from uterine cervical cancer. The interferon was produced in human diploid fibroblasts treated with Poly I:C. Anticancer drugs examined were as follows; antibiotics (aclacinomycin, actinomycin D, adriamycin, cycloheximide, mitomycin C, peplomycin, puromycin), antimetabolites (cytosine arabinoside, 5-fluorouracil, 6-mercaptopurine, methotrexate), alkylating agents (ACNU, melphalan), and others (cisplatin, hydroxyurea, vincristine). The cytotoxic effects were determined by colony formation. Our results demonstrated that interferon potentiated significantly the cytotoxic effects of peplomycin, aclacinomycin, cisplatin, 5-fluorouracil, and adriamycin on HeLa cells. The present results indicate that a combined administration of interferon and these drugs may be effective in the treatment of human cancers.

Aclarubicin↗
PubMed1982

Effects of feeder layers made of human, mouse, hamster, and rat cells on the cloning efficiency of transformed human cells.

The effect of feeder layers on cloning efficiency of transformed human cells was investigated. Embryonic human skin or lung fibroblasts; adult human skin fibroblasts; early passage cells from embryos of mouse, rat, and hamster; established mouse cell lines; 3T3 and 10T1/2 were used as feeder layers after they were lethally exposed to Co-60 gamma-rays at 3,000 rad. As test cells to study the effect of feeder layers on cloning efficiency, WI-38 CT 1 cells transformed in vitro by Co-60 gamma-rays and HGC cells cultured from a human gastric cancer were used. The effect of feeder layers on the cloning efficiency of the test cells was dependent on cell density of feeder layer cells, sources of the feeder layer cells, and kinds of test cells. An optimal density of feeder cells produced cloning efficiencies 3 to 15 times higher than in cultures without a feeder layer. Generally, high density of cells in feeder layers decreased the cloning efficiency of the test cells, presumably owing to contact inhibition of growth and depletion of essential nutrients by the feeder layer cells. Regarding the effect of the feeder layers made of human fibroblasts, there were no significant differences in population doubling levels; tissue origins of fibroblasts, or fibroblasts derived from normal individuals, patients with cancer, or with a genetically high familial incidence of cancer, hereditary adenomatosis of the colon and rectum.

Animals↗
PubMed1982

Computed tomography of mediastinal bronchogenic cysts.

The computed tomographic (CT) findings in seven cases of mediastinal bronchogenic cysts were reviewed. Two showed homogeneous high density masses with CT numbers of 97 and 89 Hounsfield units (HU). One had a CT number of 40 HU. Four cases had low density masses with CT numbers ranging from -3 to 20 HU; however, two of these four low density masses had either high density outer walls or deposits. All but one of the patients' chest radiographs revealed similar water density masses. Radiographic evidence of calcium deposits was previously considered rare, but its demonstration with CT appears to be common in mediastinal bronchogenic cysts.

Adult↗
PubMed1982

Hepatocellular carcinoma combined with hepatic mesenchymal cell tumor and sarcoma.

An autopsy case of hepatocellular carcinoma combined with sarcoma and mesenchymal cell tumor or cholangiole cell carcinoma is presented. The main tumor nodes in the liver consisted of hepatocellular carcinoma and sarcoma. Extrahepatic metastases showed characteristics of monocytic or reticulum cells and hemangioendothelial sarcomas. In this report, some problems concerning hepatocellular carcinoma and sarcomatous representation of the liver tumor are proposed. Differentiation to the monocyte-macrophage-like cells and a reticulum cell pattern were present in the metastatic locations. Thus strongly suggesting that the sarcoma may have arisen from mesenchymal cells, particularly from the Kupffer cells or endothelia consisted of the sinusoid. However, mesenchymal tumor from cholangiole cells might be associated in a part of the sarcomatous cells.

Adenocarcinoma↗