Biomedical subjects

T Kimoto

Publications and source records attributed to T Kimoto.

At least 91 records · Page 5Linked to original sources

PubMed1992

Androgen receptor in cirrhotic liver, adenomatous hyperplastic nodule and hepatocellular carcinoma in the human.

Androgen receptors (AR) were assayed for cirrhotic liver, adenomatous hyperplastic nodule (AHN), and hepatocellular carcinoma (HCC) which were removed by partial hepatic resection from five patients. There were three men and two women. Age ranged from 59 to 68 years. Underlying cirrhosis was macronodular in three and micronodular in two. AHN was present within HCC in two patients, but these two lesions were found in different lobes of the same liver in another two patients. Only AHN was seen in the last patient. ARs in the cytosol of cirrhotic liver ranged from nil to 12.8 fmol/mg of protein with the dissociation constant (Kd) of 3.2-20.3 x 10(-10) M. AHNs possessed ARs ranging from 4.0 to 27.6 fmol/mg of protein (Kd values, 7.4-21.0 x 10(-10) M. All HCC nodules had ARs ranging from 11.8 to 72.8 fml/mg of protein with Kd values of 5.3-27.1 x 10(-10) M. Thus, cytosolic AR concentrations were highest in HCC, lowest in cirrhotic liver, and intermediate in AHN. The present study seems to indicate that AHN may be a precancerous lesion and that androgen and its receptor play an important role in human hepatocarcinogenesis.

Aged↗

PubMed1992

[Evaluation of repeated hepatic dearterialization combined with intra-arterial infusion chemotherapy of unresectable primary or secondary cancer of the liver].

Repeated hepatic dearterialization combined with intra-arterial infusion chemotherapy was performed in 29 patients with unresectable primary or secondary cancer of the liver. Partial Response (PR) was obtained in 4 cases (1 hepatocellular carcinoma and 3 gastric secondaries), when evaluated by measuring the regression rate radiologically. The most remarkable effect was found in those with metastases from gastric cancer. A satisfactory result was not obtained for hepatocellular carcinoma with liver cirrhosis because of frequent associated complications. A strategy to modulate the resistance of tumors to ischemia and anticancer drugs should be considered in order to obtain a better clinical result by this method.

Catheterization↗

PubMed1991

Progesterone receptor in hepatocellular carcinoma. Correlation with androgen and estrogen receptors.

Progesterone receptors (PgR), estrogen receptors (ER), and androgen receptors (AR) were assayed consecutively for hepatocellular carcinoma (HCC) that was surgically removed from 19 men and three women. The methods of receptor assay were the enzyme immunoassay (EIA) for PgR and the dextran-coated charcoal (DCC) technique for ER and AR. The patients ranged in age from 32 to 77 years (average, 60.3 years). No patients had received any specific anti-cancer therapy before tissue collection. All patients but one had underlying liver disease: cirrhosis in 13 and chronic hepatitis in eight. The positive rate of each receptor was 18% for PgR, 48% for ER, and 82% for AR. The titer was highest for AR, intermediate for ER, and lowest for PgR. The titers of PgR in four PgR-positive patients ranged from only 1.1 to 3.0 fmol/mg of protein. There was no relationship between PgR, ER, and AR in terms of positivity and titer. Also, other clinical and histopathologic data did not influence the positivity or concentration of these three sex hormone receptors. It can be concluded that no or little PgR exists in the cytosol of untreated HCC.

Adult↗

PubMed1991

[Diagnostic ability of various biopsy needles].

We compared the diagnostic ability of various biopsy needles. A liver of an anesthetized rabbit was biopsied using 8 needles for cytology (23G, 22G, 21G Spinal; 23G, 22G, 21G Chiba; 22G Westcott; 22G Rotex II) and 12 needles for histological core biopsy (21G, 18G Sonopsy; 23G, 21G, 19G, 17G Sure-cut; 21G Majima; 18G, 16G, 14G Quick-cut; 14G Tru-cut; 18G Roth-biopsy). The volume of the recovered cells was not dependent on the bore of the needles for cytological biopsy (21-23G). However, the volume of the recovered tissue was dependent on the bore of the needles for core biopsy (14-23G). 22G Westcott needle and 22G Rotex II needle both showed excellent ability to obtain large amount of cells. 21G Sure-cut needle and 21G Majima needle both could obtain enough volume for histological diagnosis.

Animals↗

PubMed1991

Immunoelectron microscopic detection of band 3 protein during erythroid cell differentiation by a monoclonal antibody.

We created a monoclonal antibody, designated EB1 (IgM, kappa), that reacts with erythroblasts by fusion of P3-X63-Ag8.653 with splenocytes of rats immunized with erythroblastic islands isolated from mice spleens. Western blotting revealed that EB1 reacted with the band 3 protein of the erythrocytic membrane. It stained erythrocytes and erythroblasts, forming clusters in the bone marrow, splenic red pulp, and fetal liver, but did not stain other tissues in the cryostat sections. The EB1 antigen was detected during dimethyl sulfoxide-induced differentiation of murine erythroleukemia cells. Immunoelectron microscopy revealed that the EB1 antigen was expressed from the basophilic erythroblasts during normal erythroid differentiation. Preferential segregation of the EB1 antigen on the cell membrane of the nucleating erythroblasts was not observed. These results suggest that EB1 is specific for erythrocyte band 3 protein and may be useful for studying erythroid cell differentiation.

Animals↗

PubMed1991

Regulation of Forssman antigen expression during maturation of mouse stromal macrophages in haematopoietic foci.

During a trial to develop a monoclonal antibody (mAb) specific to stromal macrophages (M phi) in haematopoietic foci, we have created a mAb, designated F10, that stains the stromal M phi more selectively than any other mAb reported. As F10 was found to react with Forssman glycosphingolipid (GSL) specifically and to give clearer immunostaining than anti-Forssman GSL IgG, we have studied Forssman antigen expression during maturation of the stromal M phi in splenic haematopoietic foci using F10 and a system of allogenic bone marrow transplantation which allows us to know the turnover of the stromal M phi in vivo. C3H/He mice (H-2k) were lethally irradiated and intravenously infused with the bone marrow cells of BALB/c nu-nu mice (H-2d). Splenic frozen-sections and cytocentrifuge preparations of splenic haematopoietic clusters from the recipient mice were stained with F10 and with mAb against major histocompatibility class I antigens. H-2d-type stromal M phi began to appear in the haematopoietic clusters at Week 5 and they gradually replaced H-2k-type stromal M phi. The percentage of Forssman+ stromal M phi gradually decreased and reached a nadir at Week 6, when most stromal M phi were already of the donor type. At Week 8, however, Forssman+ stromal M phi levels returned to normal. The delayed expression of Forssman antigen on the stromal M phi in haematopoietic foci following genotypic conversion suggests that Forssman antigen is regularly expressed on the subpopulation of stromal M phi, which mature well under specific microenvironmental factors in vivo.

Animals↗

PubMed1990

Isolation and short-term culture of mouse splenic erythroblastic islands.

We isolated and cultured erythroblastic islands (EI) from the spleens of phlebotomized mice using a combination of collagenase digestion, unit gravity sedimentation, and Percoll density gradients separation. The isolated EI were composed of surrounding erythroid cells and central stromal macrophages (M phi), which were identified by Forssman antigen. While 60% of the erythroblasts incorporated bromodeoxyuridine, the M phi did not. EI could be maintained on a plastic dish for a short period in the presence of erythropoietin. Two hours later, the central M phi spread well and bound to erythroblasts via cytoplasmic processes. One day later, erythropoietic activity on the M phi surface continued, although their processes had retracted. Some EI showed synchronized expansion of erythroblasts and others showed differentiation to reticulocytes. Two days later, about 50% of the EI still showed erythropoietic activity and most erythroblasts differentiated to the orthochromatic stage. On the other hand, the M phi secreted colony-stimulating activity during the culture. It was infrequently observed that erythroid and myeloid populations simultaneously expanded on a central M phi. These results indicate that this EI culture system is useful for studying interactions between the stomal M phi and hematopoietic cells.

Acid Phosphatase↗

PubMed1990

Clinicopathologic features and long-term results of alpha-fetoprotein-producing gastric cancer.

During a 10-yr-period, 24 cases of alpha-fetoprotein-producing gastric cancer were experienced in our department. The mean age was 62.5 yr, and the sex ratio of males to females was 3:1. Borrmann II and III types of gastric cancer were predominant (83.3%). The prognosis was dismal. Most of the patients, including three radically operated cases of early gastric cancer, died from liver metastasis within 2 yr. The 1-, 3-, and 6-yr survival rates were 37.5%, 8.3%, and 8.3%, respectively, for all cases and 75.0%, 25.0%, and 25.0% for radically operated cases. The incidences of synchronous and metachronous liver metastasis were 31.8% and 40.9%, significantly higher than the incidences of AFP-negative gastric cancer (p less than 0.91). Despite radical gastrectomy, metachronous liver metastasis occurred in 75.0% of the cases. Two radical hepatic resections, including extended right lobectomy, were performed in one patient with early gastric cancer who had repeated metachronous liver metastasis. However, the tumor recurred immediately. Apparently, radical gastrectomy or hepatic resection alone may not suffice for this particular type of cancer. The methods of treatment and follow-up considered should be different from that for other types of gastric cancer.

Adult↗

PubMed1990

[A case of lateral thoracic meningocele without neurofibromatosis].

A case of lateral thoracic meningocele without neurofibromatosis is reported. CT-myelography revealed the mass has the communication with CSF space. So we could differentiate it from a posterior mediastinal tumor. It is important to think of the presence of lateral thoracic meningocele when any posterior mediastinal mass is detected.

Adult↗

PubMed1990

[New devices for non-vascular interventional radiology].

Interventional Radiology (IVR) is a field of medicine, which has made diagnosis or treatment previously performed only by surgery possible percutaneously. For safe and successful IVR, good devices are necessary. Therefore, development of new devices is essential for the progress of IVR. Various kinds of new devices for non-vascular IVR are introduced, including new biopsy needles, AccuStick, Cope Gastrointestinal Suture, Rösch-Thurmond Fallopian Tube Catheterization Set, ESP Glove and others. Most of them are now commercially available in Japan. However, all of them cost much higher than they cost in the USA.

Humans↗

PubMed1989

Establishment of five human myeloma cell lines.

Five human myeloma cell lines, KMM-1, KMS-5, KMS-11, KMS-12-PE, and KMS-12-BM, have been established at Kawasaki Medical School since 1980. As the KMS-12-PE and KMS-12-BM lines were obtained from the same patient, these five cell lines have been derived from four patients with multiple myeloma. The five myeloma cell lines are stably growing at present in RPMI 1640 medium supplemented with 10% fetal bovine serum. They can also grow in a defined culture medium without serum. That these cell lines were human myeloma cells was confirmed by the following findings. Ultrastructurally, all five cell lines showed features characteristic of plasma cells. KMM-1 and KMS-11 cells secreted lambda and kappa chains into the culture medium, respectively, but the other cell lines produced no immunoglobulins. KMM-1 expressed cytoplasmic lambda antigen, KMS-5 showed cytoplasmic delta, and KMS-11 expressed surface kappa, whereas KMS-12-PE and KMS-12-BM cells showed no surface or cytoplasmic immunoglobulins. Regarding reaction with a monoclonal plasma cell antibody (PCA-1), four of the five lines were positive, the exception being KMS-5. Another monoclonal antibody (CD38), which also recognizes plasma cells, responded to KMM-1, KMS-12-PE, and KSM-12-BM. KMS-5 cells expressed acute lymphoblastic leukemia antigens (CALLA). These data suggest that such lines as KMM-1, KMS-11, KMS-12-PE, and KMS-12-BM represent later stages of B-cell differentiation, and that KMS-5 represents a relatively early stage of B-cell differentiation. All the cell lines lacked Epstein-Barr virus nuclear antigen, showed abnormal karyotypes of human origin, and differed from each other in the isozyme patterns examined. Only KMS-5 was tumorigenic when transplanted subcutaneously into nude mice.

Aged↗

PubMed1989

Participation of radioresistant Forssman antigen-bearing macrophages in the formation of stromal elements of erythroid spleen colonies.

Using immunohistological methods with anti-Forssman glycosphingolipid antiserum and F4/80 monoclonal antibody, we investigated the dynamics of stromal macrophages during the development of haematopoietic spleen colonies in lethally irradiated adult mice which had been transplanted with syngeneic bone marrow cells. After irradiation, the spleen had shrunk and both Forssman +F4/80+ and Forssman-aggregated F4/80+ macrophages tended to aggregate in the red pulp. On the other hand, after irradiation and transplantation, discriminate erythroid foci developed in the shrunken splenic red pulp and both types of aggregating macrophages, which were involved in the foci, began to extend their cytoplasmic processes. Later, the macrophages distributed themselves within the enlarged erythroid colonies and showed fine cytoplasmic extensions among erythroblasts. We also examined the occurrence of stromal macrophages in the haematopoietic foci during spleen development. While Forssman + macrophages were scarcely present at birth when erythropoietic activity had already occurred, many F4/80+ macrophages were observed. Forssman + macrophages gradually increased in number up to 3 weeks after birth. The results clearly demonstrated heterogenicity of stromal macrophages in haematopoietic foci.

Animals↗

PubMed1989

[Finishing and polishing of composite resins. The experimentally designed silicone cup hards and their polishing ability].

To establish the most efficient polishing technique for composite resins, four kinds of silicone cup hards were experimentally designed, and the surface texture of two semihybrid composite resins polished with these experimental polishing tools were examined using a surface roughness recorder and by SEM. The individual silicone cup hard consisted of a hard rubber and silicone carbide abrasive particles being sized into #180 (P0), #360 (P2), #600 (P3), and #2500 (P4), respectively. Combination polishing with both P3 and P4, as well as from P0 through P4 in this order, efficiently created the smoothest surfaces for the semihybrid composite resins, which are generally considered to be hard to polish in the routine clinic.

Composite Resins↗

PubMed1989

[Establishment of a composite resin inlay technique. Part 1. The effects of various curing modes on mechanical properties of composite resins].

The effects of the curing mode on mechanical properties of composite resins were examined. Four resins as inlay, and three chemically-cured and five visible light-cured restorative resins were employed. The resin specimens were prepared by three kinds of curing modes; regular setting (according to the manufacturer's instruction), subsequently added light and heat curing after regular setting, and subsequently added heat and pressure curing after regular setting. Knoop hardness, flexure strength, compressive strength, and diametral tensile strength were determined. All restorative composites were remarkably increased in knoop hardness number due to the subsequently added curing methods. Both subsequently added curing methods provided higher flexure strength to all restorative resins, and particularly in the chemically-cured resins the flexure strength provided by the subsequently added light and heat curing was higher than those by the subsequently added heat and pressure curing. Compressive strength and diametral tensile strength were slightly increased by the subsequently added curing methods with the restorative resins. No correlation was found between the filler distribution and the mechanical properties provided by the subsequently added curing methods. The subsequently added heat curing seems to be preferable for creating higher mechanical properties of resins. The IC-2 resin, experimentally designed for resin inlay, seems to be the most promising resin for inlay restoration, based on the mechanical properties, and further detailed laboratory and clinical researches are required.

Composite Resins↗

PubMed1988

Multi-step neoplastic transformation of normal human fibroblasts by Co-60 gamma rays and Ha-ras oncogenes.

As reported previously (Namba et al., 1985; Namba, 1985), normal human fibroblasts were transformed into immortal cells with abnormal karyotypes by Co-60 gamma-ray irradiation. These immortally transformed cells (KMST-6) showed no clonability in soft agar and were not tumorigenic. However, by treatment with Ha-ras oncogenes derived from a human lung carcinoma or Harvey murine sarcoma virus, the KMST-6 cells acquired elevated clonability in soft agar and transplantability in nude mice. All the tumors produced grew progressively without showing regression and killed the mice. The tumors were also serially transplantable into other mice. The Ha-ras oncogene alone did not convert normal human fibroblasts into either immortal or tumorigenic cells. Our current data suggest that gamma rays worked as an initiator of carcinogenesis in normal human cells, giving rise to chromosome aberrations and immortality, and the Ha-ras oncogene played a role in the progression of the immortally transformed cell population to a neoplastic one showing enhanced colony formation in soft agar and tumorigenicity in nude mice.

Cell Line↗

PubMed1988

Fibroblasts of two patients with trisomy 18 show 1.5-fold increase in peptidase A activity over normal human diploid fibroblasts.

Peptidase A (Pep A) activity assigned to chromosome 18q23 was biochemically examined in fibroblasts cultured from two patients with trisomy 18 and in fibroblasts derived from normal individuals. The trisomy 18 fibroblasts showed approximately a 1.5-fold increase in Pep A activity over that of the control fibroblasts. Agar gel electrophoretic analysis revealed no detectable differences in the electrophoretic mobility and isoenzyme patterns of Pep A between the trisomy 18 fibroblasts and normal ones. The present results show the trisomy 18 fibroblasts to be suitable for study of the gene dosage effect of Pep A.

Aspartic Acid Endopeptidases↗

PubMed1988

[A case of cystadenocarcinoma of the liver complicated by gastric cancer--a review of 83 cases of primary cystic liver cancer in the literature].

So far as is known, this is the first reported case in the literature of a primary cystic liver cancer complicated with a gastric cancer. A 77-year-old female was admitted to hospital because of a swelling in the upper abdomen and appetite loss. CT scanning, an echogram, and an angiogram of the abdomen revealed a large cystic lesion which originated from the left lobe of the liver. A DIC indicated a gall stone, Further, upper GI fluoroscopy and endoscopy revealed a gastric cancer of the Borrmann II type, and thus a dome resection of the cyst, cholecystectomy, aad 3/4 partial gastrectomy was performed. The liver was found to contain much mucin. The histology indicated a cystadenocarcinoma of the liver, and an adenocarcinoma of the stomach of a poorly differentiated type.

Aged↗

PubMed1988

[Two color analysis of peripheral lymphocyte subsets in patients with cancers during and after radiotherapy].

Lymphocyte subsets of peripheral blood in patients with cancers were analyzed through two color analysis using FCM. Leu-2/Leu-3 and Leu-2+15+/Leu-8-3+ were compared during radiotherapy and periods of follow up in cases with uterine cervical ca. and lung ca. They did not show similar tendencies but just a opposite one in some cases. Both of them, on the other hand, did not appear to be related with the prognosis of patients. However, CTL and NK (potent) did show a relevance with it through the period of follow up. They might be indices which allow to predict the clinical course of Patients with cancers. Further study is now in progress.

Adult↗