Biomedical subjects

T Kikuchi

Publications and source records attributed to T Kikuchi.

867 records · Page 49Linked to original sources

PubMedDate not supplied

Post-transfusion graft-versus-host disease following open heart surgery. Report of six cases.

Six cases of post-operative erythroderma after open heart surgery are described. About 10 days after seemingly uneventful recovery, all patients developed fever, erythroderma, liver enzyme elevation, pancytopenia, and an aplastic bone marrow. Their condition rapidly deteriorated, and they died within 20 days of the onset of symptoms. Skin biopsy specimens from two patients showed mild leukocytic infiltration in the epidermal basal layer and upper dermis. Immunostaining by the ABC method showed that most of these infiltrating cells were suppressor/cytotoxic T cells. HLA study of peripheral lymphocytes from two patients and their families revealed that the patients' HLA phenotypes were incompatible from their children's HLA findings. Y chromatin was present in the lymphocytes of the skin biopsy specimen of a female patient. Based on the clinical picture, skin biopsy, HLA study, and Y chromatin study, the authors strongly suspect post-transfusion GVHD as the etiology of postoperative erythroderma, although these patients lacked any known immunodeficiency.

Adult↗

PubMedDate not supplied

Cytophotometric study of premalignant and malignant cells of the cervix in an approach towards automated cytology.

Based on a cytophotometric procedure in combination with Feulgen reactive DNA and Pap-1 labeled specimens, the DNA values and hematoxylin-dense substances were examined in individual cells from 62 cases of premalignant and malignant lesions of the cervix, in contrast to normal squamous epithelia of 40 cases and 500 normal lyphocytes. The range of DNA values for the cells from benign proliferation and mild dysplasia cases was within a normal diploid-tetraploidy. In contrast, cases with carcinoma in situ and microinvasive carcinoma exhibited a DNA range which extended beyond hypertetraploidy with many cellspossessing a DNA value in excess of hexaploidy. Marked dysplasia from 11 cases also showed the same DNA range as carcinoma in situ. Our decision criteria derived from thresholding DNA value may be useful in an automatic cancer diagnostic test. In an aid to the manual measurement, an automatic glass fibrooptic system is now in preparation in an approach towards automation of uterine cytology for scanning of malignant cells.

Carcinoma in Situ↗

PubMedDate not supplied

Stimulus induced repetitive muscle potentials in the gracile axonal dystrophy (GAD) mouse.

Gracile axonal dystrophy (GAD) is an inherited neurodegenerative disease in the mouse with progressive sensory ataxia and motor paresis. Electromyographic examination was conducted in 25 unanaesthetized GAD mice and 24 controls of the same strain during 6, 9 and 12 wk of postnatal life. Among 9 and 12 wk old mice, about 75% showed resting spontaneous activities--either fibrillation or fasciculation or both. On stimulation of the tibial nerve at ankle, with single pulse, the EMG showed repetitive muscle potentials of large amplitude, following "M" response. The frequency and duration of the train of these stimulus--induced repetitive muscle potentials (SIRMP) were almost constant in a given animal. The SIRMP failed to reappear in response to the second stimulus within 5 s when twin pulses were applied or within 5 min when tetanic stimuli were applied, indicating their fatigability. It is concluded that the SIRMP originate from immature, supplementary motor endplates that develop at ultraterminal nerve sproutings induced by denervation and reinnervation and possibly are due to hyperexcitable trigger points in the peripheral nerve endings. The EMG abnormalities in the GAD mouse are very much similar to those observed in human syndromes with hyperexcitable peripheral nerves that result in sustained muscle activity like neurotonia and hence the GAD mouse is a good model of such motor abnormalities in man.

Action Potentials↗