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Biomedical subjects

T Kanno

Publications and source records attributed to T Kanno.

At least 613 records · Page 34Linked to original sources
PubMed1977

[Classification of intracerebral hematoma (lateral type) on C.T. and its relation to the shift of the lenticulostriate arteries (author's transl)].

Classification of intracerebral hematoma (lateral type) with C.T. was tried. And also, the relationship among the location of the hematoma, the shift of the lenticulostriate arteries and the prognosis of hemiparesis was verified. The results were as follows; 1) The location of the hematoma was classified into four types: 1. anterior type (the hematoma mainly located in the anterior limb of internal capsula). 2. Posterior type (the hematoma mainly located in the posterior limb of internal capsula). 3. lateral type (the hematoma mainly located just below the insula). 4. spread to the all direction type. 2) The lenticulostriate arteries was divided into three portions: L1 portion is the part from the origin at the middle cerebral artery to the knee point, L3 is the part of the distal portion of the lenticulostriate arteries, and L2 portion in the part between L1 and L3 portions. L1 portion was mainly shifted medially in the anterior type of the hematoma. L2 portion was mainly shifted medially in the lateral type of the hematoma. L3 portion was mainly shifted in the posterior type of the hematoma. L1, L2, L3, all portions were shifted medially in the type in which the hematoma spread to all directions 3) The prognosis of the hemiparesis was best in the lateral type of the hematoma, and next in the anterior type. In the posterior type and all spread type, it was worst.

Cerebral Angiography↗
PubMed1976

Properties of the amylase produced in carcinoma of the lung.

The properties of the amylase produced by carcinoma of the lung were studied. The abnormal amylase recognized in the serum of a patient with carcinoma of the lung had a mobility with beta-position and showed reduced migration to the cathodic side after neuraminidase digestion. This abnormal amylase had a close affinity for Concanavalin A and this affinity was not retarded by the neuraminidase digestion. However, the purified, tumor-extracted, amylase from the same patient had the same electrophoretic migration as normal human salivary amylase and was not affected by neuraminidase treatment. The abnormal affinity for Concanavalin A was not observed in this purified tumor-extracted amylase. It is suggested that some transglycosidation steps are needed for the appearance of the abnormal amylase in the patient's serum, and that the terminal sialic acid is independent of the affinity for Concanavalin A. The dissociation constants of the tumor amylase for several substrates were smaller than those of normal pancreatic or salivary amylases. Moreover, maltotriose had no affinity for the tumor-extracted amylase and it was not digested to maltose and glucose by the purified tumor extracted amylase. These differences in the kinetic properties and in the mode of digestion were of interest in the study of tumor-produced amylases.

Adenocarcinoma↗
PubMed1976

Stimulus-secretion coupling in pancreatic acinar cells: inhibitory effects of calcium removal and manganese addition on pancreozymin-induced amylase release.

The role of Ca ions in stimulus-secretion coupling has been analysed in the isolated and perfused rat pancreas. 2. The omission of [Ca2+]O diminished but did not abolish the release of amylase in response to continuous stimulation with 5 m-u. pancreozymin (Pz)/ml. The addition of Mn2+ (1-0 mM) to this Ca-deficient environment abolished the residual release of amylase. This was followed by a complete recovery of amylase output when the control [Ca2+]O was reestablished. 3. The addition of Mn2+ (1-0 mM) to the extracellular environment containing 2-5 mM-Ca2+ reversibly inhibited the Pz-induced release of amylase. 4. A kinetic scheme based on competition of Ca and Mn at a carrier in the acinar cell membrane could quantitatively explain the effects of Ca and Mn upon the Pz-induced amylase release. 5. These results support the view that the Ca2+ influx into the acinar cells is the major contributor to the rise in [Ca2+]i which, in turn, mediates the processes in the stimulus-secretion coupling in the exocrine pancreas, and suggest that the mode of Ca influx is a facilitated diffusion.

Amylases↗
PubMed1976

Progressive enhancement in the secretory functions of the digestive system of the rat in the course of cold acclimation.

1. The secretory function of the exocrine pancreas and the stomach have been studied in the course of cold acclimation of rats that had been fed at an ambient temperature of 1 degree C in a climatic room. 2. The secretory responses of pancreatic enzymes evoked by continuous infusion of pancreozymin (PZ, 2-5 mu./kg. hr) and a rapid single injection of PZ (1.7 mu./kg) reached a maximum in the group of rats fed at 1 degree C for 4 weeks, and fell to the control levels after 8 weeks. The increase in the flow of pancreatic juice evoked by single injection of PZ was maximal at 4 weeks and slightly decreased after 8 weeks. 3. The insulin (3-0 i.u./kg) evoked secretion of pancreatic enzymes gradually increased after cold exposure, reached a maximum at 4 weeks and fell to the control levels after 8 weeks. The flow of pancreatic juice after insulin injection was almost the same in every group throughout the course of cold exposure. 4. The ratio of amylase to the total amount of the protein in the pancreatic juice decreased abruptly, in contrast to an increase in the ratio of protease in the process of cold acclimation. The change in the ratio of enzyme activity in the pancreatic juice may reflect parallel changes in enzyme activity in the exocrine pancreas. 5. The gastric secretion in response to insulin and bile secretion in the group fed at 1 degree C for 7 weeks was significantly higher than that in the control group. 6. It was thus concluded that the secretory activities of digestive system were enhanced by prolonged cold exposure and then returned to control level, and that the activites of the pancreatic enzymes were altered in the process of cold acclimation in rats.

Acclimatization↗
PubMed1976

The potentiating influences of insulin on pancreozymin-induced hyperpolarization and amylase release in the pancreatic acinar cell.

1. Insulin (1 mu./ml.) potentiated the release of amylase from isolated pancreas of rats perfused with erythrocyte-containing medium and stimulated by 0-5 m-u. pancreozymin (Pz)/ml., whereas the same concentration of insulin failed to potentiate the response evoked by or 200 m-u. PZ/ml. 2. Intracellular measurement of membrane potentials from the acinar cells of the same preparations showed that insulin (1 mu./ml.) simultaneously potentiates the hyperpolarization and amylase release in response to 0-5 m-u. PZ/ml. 3. These effects of insulin on the PZ-induced responses were inhibited by ouabain (3 X 10(-5) M). 4. The results suggest that insulin, in the concentration studied, has a potentiating action on the activity of an electrogenic Na pump, which maintains the hyperpolarization and amylase release during continuous stimulation with Pz. 5. Insulin in also potentiated the Pz-induced amylase release in the rat pancreata in situ after vagotomy and ligature of the pyloric region.

Amylases↗
PubMed1976

Reduction of the hypoxia-induced depression in the intracellular electrical activity of the ventricular muscle fibers of the rabbit fed on food containing Crataegutt.

The rabbits were divided at random into 2 groups: one was fed for 6 weeks on the rabbit food containing 5 mg Crataegutt per 100 Gm food (CR group), and the other was fed for the same period on the commercial rabbit food and used as a control. Intracellular records were made from fibers in the ventricle of isolated rabbit heart. The inhibitory influences of hypoxia on the resting membrane potential and on the amplitude of action potential of the fiber of the CR group are significantly less than those of the control group. However, the inhibitory influence on the time courses of contraction of the papillary muscle was almost the same between these 2 groups.

Action Potentials↗
PubMed1976

Effects of oxygen supply on electrical and secretory responses of humorally stimulated acinar cells in isolated rat pancreas.

A technique is described for perfusing isolated rat pancreas with a modified Krebs-Henseleit solution. This preparation made feasible the simultaneous measurement of transmembrane potential, effective membrane resistance, rate of flow of pancreatic juice and amylase output. The hyperpolarizing effect of cholecystokinin-pancreozymin (CCK-PZ) was confirmed by perfusion with a solution containing 5 mU CCK-PZ/ml. The hyperpolarizing efffect of CCK-PZ was enhanced when the pancreas was perfused with an oxygenated solution containing dog erythrocytes. This effect was inhibited by ouabain and anaerobic conditions. It is suggested that the oxidative metabolism supplies energy for a mechanism which is responsible for the hyperpolarizing effect of CCK-PZ. The amylase output and the rate of flow of pancreatic juice were enhanced concurrently under conditions that augumented the hyperpolarizing effect, and they were inhibited under conditions that suppressed the effect. Cellular events in stimulus-secretion coupling in the pancreatic acinar cell are discussed by correlating the electrophysiological responses and the secretory responses under various conditions.

Amylases↗
PubMed1975

Sialic acid containing abnormal amylases in human sera.

The properties of abnormal amylases with unusually fast electrophortetic mobility from five patients were studied. These abnormal amulases showed markedly reduced electrophoretic migration to the cathodic side by digestion with neuraminidase. It was suggested that these five abnormal amylases contained sialic acid in their molecules, and the increased electrophorectic mobility resulted from sialic acid, which is not present in normal human amylases.

Amylases↗
PubMed1975

The electrogenic sodium pump in the hyperpolarizing and secretory effects of pancreozymin in the pancreatic acinar cell.

1. Transmembrane potential, effective membrane resistance, and amylase release were recorded simultaneously from acinar cells of isolated rat pancreas perfused with Krebs-Henseleit solution. 2. The hyperpolarizing effect of pancreozymin (Pz) was confirmed by perfusing with a solution containing 5 m-u. Pz/ml. 3. The hyperpolarizing effect of Pz disappeared in the following environments: (a) in low ambient temperature, (b) in K-free medium, (c) in low Na medium, and (d) in the presence of ouabain. In these environments, transient depolarization was frequently observed immediately after stimulation. It is suggested that there are two components in the effect of Pz on the membrane potential of the acinar cells: transient depolarization which coincides with an increase in Na permeability in the initial phase, and continuous hyperpolarization due to an electrogenic Na pump which conceals transient depolarizing phase when the pump is dominant. 4. The secretory effect of Pz was inhibited under conditions that suppressed the electrogenic Na pump. It is proposed that the Na pump activated by Pz maintains the passive Na-influx, increases [Ca2+]i, and, in consequence, uphold the amylase output during continuous stimulation. 5. A medium which was used to bathe the sectioned pancreas with Pz was found to contain a substance which depolarized the acinar cells.

Amylases↗
PubMed1975

Rabbit's ear in cold acclimation studied on the change in ear temperature.

The role of the rabbit's ear in cold acclimation was studied by varying the temperature of a climatic room in the range from -10 to +30 degrees C; The skin temperature in a nonanesthetized rabbit's ear showed a characteristic response to changes in ambient temperatures; plotting the ear temperature against the ambient temperature yielded an S-shaped curve. The mean ambient temperature corresponding to the inflection point on the S-shaped curve shifted significantly from about 13 degrees C to about 8 degrees C after cold acclimated of a group fed for 7 wk at -10 degrees C. The shift of the S-shaped curve after cold acclimation may not be due to the change in the norepinephrine sensitivity of the vascular beds of the ear: the effect of norepinephrine on the pressure-flow curve in the isolated rabbit's ear was almost unchanged between the control and the cold-acclimated groups. It is proposed that the shift of the inflection point gives a qualitative index of the acclimated state of the rabbit at a particular temperature.

Acclimatization↗