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Biomedical subjects

T Kanno

Publications and source records attributed to T Kanno.

At least 559 records · Page 31Linked to original sources
PubMed1981

Bivariate ratio monitoring in clinical laboratories.

The usefulness of monitoring bivariates was studied and discussed. Analysis of the correlation between bivariates was studied and discussed. Analysis of the correlation between bivariates demonstrated that combinations of bivariates can be divided into two groups. The first group was the combination in which a close correlation between two variates could be observed in any pathophysiologic state. The monitoring of the ratio of this first group was effective for the detection of analytical mistakes and the detection of symptom poor hereditary disorders, such as pharmacogenetical disorders. The second group was one in which a high correlation between two variates was obtained in some specific pathologic stage, and the monitoring the ratio of this second group gave high predictive values for the screening of that pathologic stage. It was demonstrated that the use of bivariate monitoring was not only very simple but also provided an effective laboratory data analysis system for the laboratory routine.

Aspartate Aminotransferases↗
PubMed1980

Hereditary deficiency of lactate dehydrogenase M-subunit.

A family with a complete deficiency of lactate dehydrogenase M-subunit was investigated. The propositus was an 18-year-old male who complained of exertional pigmenturia and easy fatigue. Marked discrepancy was observed in the ratio between creatine kinase and lactate dehydrogenase (CK/LDH). Electrophoretic analysis of serum LDH isoenzymes of the propositus demonstrated only one activity band of LDH H4. A complete lack of the LDH M-subunit was similarly demonstrated in erythrocytes, leukocytes and in the intermediate vastus muscle. LDH levels in the muscle specimen were markedly decreased in the patient, whereas CK and aspartate aminotransferase were almost the same as in a control subject. LDH isoenzymes of erythrocytes were analyzed in 5 siblings and in the parents. This demonstrated a complete lack of LDH M-subunit in 3 siblings. The ratio between H-subunit and M-subunit (H/M) in erythrocyte LDH suggested a partial absence of the M-subunit in two siblings and in the parents. An abortive increase of blood lactate and a marked increase in blood pyruvate were observed immediately after ischemic work of the forearm, accompanied by an increase in serum creatine kinase and myoglobinuria. The present case represents a newly described form of genetically determined myopathy.

Adolescent↗
PubMed1980

Membrane associated enzyme complexes containing lambda type immunoglobulin A.

The properties of the membrane associated enzyme complexes were investigated. The enzyme complexes of alkaline phosphatase, arylamidase, and L-gamma-glutamyltranspeptidase were excluded by gel filtration on Sephadex G-200. The electrophoretic mobility of the enzyme complexes was found to be in the alpha-1 portion and these enzyme activities were immunologically fixed by anti-IgA and anti-light chain lambda antibodies. Heterogeneity in density was observed by density gradient ultracentrifugation. The activities of membrane-associated enzymes were demonstrated in both high (d > 1.21) and low density particles (1.063 < d < 1.21), and the enzyme activities of a high density particle were immunologically fixed by anti-IgA and anti-light chain lambda antibodies. The heterogeneity in density was based on the presence of immunoglobulin A molecule in the complexes. The specificity of the immunoglobulin which was found in the enzyme complexes proved to be exclusively of the alpha heavy chain classes and lambda light chain types respectively. This isotype specificity of immunoglobulin A is similar to the specificity observed in enzyme-linked immunoglobulins, and simple non-specific binding of immunoglobulin and membrane moiety was eliminated. This isotype specificity is of interest in connection with the properties of the circulating autoantibodies.

Adult↗
PubMed1980

Potentiation of cholecystokinin-induced exocrine secretion by both exogenous and endogenous insulin in isolated and perfused rat pancreata.

Using an isolated perfused rat pancreas preparation, the interrelationship between the endocrine and exocrine portions of the pancreas were studied. Addition of exogenous rat insulin (1-20 mU/ml) to the perfusing solution potentiated the action of cholecystokinin (CCK) (1 mU/ml) to increase both pancreatic juice flow and the release of the enzyme, amylase. Raising the glucose concentration in the perfusing solution from 2.5 to 17.5 mM both increased endogenous insulin release and potentiated the CCK-induced exocrine secretory response. Two lines of evidence indicated that this effect of glucose on the exocrine pancreas was mediated by endogenous insulin release. First, the addition of comparable amounts of xylose or galactose to the perfusion medium neither released insulin nor potentiated the CCK-induced response. Second, epinephrine blocked the effect of high glucose on both insulin release and potentiation of CCK action. Epinephrine alone did not affect the action of CCK. The magnitude of the exocrine response induced by high glucose was comparable to that of 2.5 mU/ml exogenous insulin. It seems possible that pancreatic acinar cells can be exposed to insulin levels of this magnitude in situ.

Animals↗
PubMed1980

A comparison of secretory actions of VIP, secretin and CCK-PZ in the isolated and perfused kitten pancreas.

Secretory actions of VIP (vasoactive intestinal peptide), secretin and CCK-PZ (Cholecystokinin-pancreozymin) were compared in the isolated and perfused kitten pancreas. Perfusion of the isolated kitten pancreas with a solution containing 20 mU/ml CCK-PZ resulted in gradual hyperpolarization of the pancreatic acinar cell. CCK-PZ induced a dose-dependent increase in the pancreatic protein output and a small but definite increase in the flow of pancreatic juice which was also dose dependent upon CCK-PZ. Secretin and VIP induced a dose-dependent increase in the flow but a negligible increase in the pancreatic protein output. The estimated ED50 for VIP was 1.5 nM which was 5 times as large as the estimated ED50 for secretin. A considerable amount of VIP, which corresponded roughly to the activity of exogenous 0.6 nM VIP, was spontaneously released from the isolated kitten duodenum into the portal vein, and this VIP release was nullified during vascular perfusion with a Ca-deficient solution. With respect to these and other results, we will propose a view concerning a possible role of VIP in controlling the function of the exocrine pancreas.

Animals↗