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Biomedical subjects

T Kanno

Publications and source records attributed to T Kanno.

At least 397 records · Page 22Linked to original sources
PubMed1990

Prospective study of early detection of hepatocellular carcinoma in patients with cirrhosis.

We prospectively monitored 140 cirrhotic patients for the development of hepatocellular carcinoma for 6 yr, using periodical screening by high-resolution convex-array ultrasonography and alpha-fetoprotein. Twenty-eight patients were positive for HBs antigen, 26 patients had received blood transfusions and were negative for HBs antigen and 26 patients had a history of heavy drinking. We detected hepatocellular carcinoma in 40 patients during this period. The overall cumulative incidence of hepatocellular carcinoma in the 6 yr was 39%; the cumulative incidence was 59% in patients with HBsAg, 53% in patients who had had blood transfusions and were negative for HBsAg and 22% in patients who had a history of heavy drinking and who were without HBsAg. Detection of the carcinoma in 85% of these 40 patients was based on results of ultrasonography. Twenty-six of the patients (65%) had a small hepatocellular carcinoma of 2 cm or less. alpha-Fetoprotein levels were lower than 100 ng/ml in 56% of these 40 patients. Patients with cirrhosis are at high risk of developing hepatocellular carcinoma, especially patients with HBsAg or with a history of blood transfusion who are negative for HBsAg. Periodic monitoring by use of ultrasonography in particular is recommended for early detection of hepatocellular carcinoma.

Adult↗
PubMed1990

Chloride and potassium channels in U937 human monocytes.

Ionic channels in a human monocyte cell line (U937) were studied with the inside-out patch-clamp technique. A Ca2(+)-activated K+ channel and three Cl- -selective channels were observed. The Ca2(+)-activated K+ channel had an inward-rectifying current-voltage relationship with slope conductance of 28 pS, and was not dependent on membrane potential. Among the three Cl- channels, an outward-rectifying 28-pS channel was most frequently observed. The permeability ratio (Cl-/Na+) was 4-5 and CH3SO4- was also permeant. The channel became less active with increasing polarizations in either direction, and was inactive beyond +/- 120 mV. The channel, observed as bursts, occasionally had rapid events within the bursts, suggesting the presence of another mode of kinetics. Diisothiocyanatostilbene-disulfonic acid (DIDS) blocked the channel reversibly in a dose-dependent manner. The second 328-pS Cl- channel had a linear current-voltage relationship and permeability ratio (Cl-/Na+) of 5-6. This channel became less active with increasing polarizations and inactive beyond +/- 50 mV. DIDS blocked the channel irreversibly. The channel had multiple subconductance states. The third 15-pS Cl- channel was least frequently observed and least voltage sensitive among the Cl- channels. Intracellular Ca2+ or pH affected none of the three Cl- channels. All three Cl- channels had a latent period before being observed, suggesting inhibitory factor(s) present in situ. Activation of the cells with interferon-gamma, interferon-alpha A or 12-O-tetradecanoylphorbol-13-acetate (TPA) caused no change in the properties of any of the channels.

4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid↗
PubMed1990

Effects of histamine on slowly adapting pulmonary stretch receptor activities in vagotomized rabbits.

Thirty-two SAR fibers located below the carina were used in 32 anesthetized, bilaterally vagotomized, and artificially ventilated rabbits. After intravenous administration of histamine (20, 40, and 80 micrograms/kg, n = 13) or ACh (10, 20, and 40 micrograms/kg, n = 13), SARs became active during expiration but decreased their inspiratory activity. The effects of both drugs were dose-dependent. The injection of histamine or ACh at all doses examined had no effect on tracheal pressure (PT). Atropine (3 mg/kg) and isoprenaline (100 micrograms/kg) blocked the responses of SAR activity to low doses of histamine (20 and 40 micrograms/kg) and to all doses of ACh. The response of SARs to 80 micrograms/kg of histamine was not altered by atropine (n = 10) nor by isoprenaline (n = 10). These results suggest that low-dose effects of histamine on SARs occur as the result of ACh release whereas with high doses the effect of histamine on the receptors is mainly independent of ACh released from the nerve endings. In another series of experiments (n = 6) where animals were treated with isoprenaline (100 micrograms/kg) and, subsequently, physostigmine (200 micrograms/kg), histamine (20 and 40 micrograms/kg) or ACh (10 and 20 micrograms/kg) increased the inspiratory discharge in SARs and the level of PT. From these results, it is assumed that the changes of SAR activity following histamine injection in vagotomized animals reflect a local bronchomotor effect which takes place in a peripheral bronchial tree that does not affect the level of PT. Histamine seems to release ACh and to elicit bronchoconstriction that can be manifested by physostigmine treatment.

Acetylcholine↗
PubMed1990

Rapid enzymatic analysis of plasma for tyrosine.

In this rapid, simple, and convenient enzymatic method for measurement of tyrosine in plasma, tyrosine is converted to tyramine by action of tyrosine decarboxylase (EC 4.1.1.25) and the tyramine produced is oxidized to p-hydroxybenzyl aldehyde and hydrogen peroxide by action of tyramine oxidase (EC 1.4.3.9). The hydrogen peroxide is reacted with 4-aminoantipyrine and N-ethyl-N-(2-hydroxy-3-sulfopropyl)-m-toluidine in the presence of peroxidase (EC 1.11.1.7) to obtain quinoneimine dye, the absorbance of which is measured at 570 nm. Thus tyrosine is measured in the visible range. The CV was 4.6% or less, and the measurement was unaffected by other amino acids, except for phenylalanine. The values obtained (y) correlated well with those obtained with an amino acid analyzer (x): y = 0.902x + 3.92 mumol/L (Syx = 12.3; r = 0.985; n = 54).

Autoanalysis↗
PubMed1990

Cerebral hemodynamics in patients with chronic obstructive carotid disease by rCBF, rCBV, and rCBV/rCBF ratio using SPECT.

To evaluate cerebral hemodynamics, 21 patients with chronic occlusion or severe stenosis of the internal carotid or middle cerebral artery with normal or only lacunar infarction on x-ray CT were studied using single photon emission computed tomography (SPECT). We measured rCBV with 99mTc erythrocytes after rCBF with 133Xe, and calculated rCBV/rCBF. rCBF and rCBV of the 25 affected hemispheres were classified as (a) patients with normal rCBF [type I (n = 7) and type II (n = 3)]; (b) patients with decreased rCBF [type III (n = 6) and type IV (n = 9)]. These two groups then could be subdivided according to findings of rCBV, normal, and increased blood volumes. rCBV/rCBF increased as the cerebral perfusion pressure dropped from type I to type III. In type IV, other situations but cerebral autoregulation could be assumed. rCBV/rCBF signifies vascular mean transit time. Type III (high rCBV/rCBF) assumed as the increased OEF, misery perfusion as reported in PET. We propose rCBF, rCBV and rCBV/rCBF using SPECT can be an index for cerebral circulatory reserve.

Arterial Occlusive Diseases↗
PubMed1990

Increased activities of cytosol aminopeptidase and lactate dehydrogenase in serum originate from lymphocytes in necrotizing lymphadenitis.

In three pediatric patients with necrotizing lymphadenitis, cytosol aminopeptidase activity (c-AP; EC 3.4.11.1) in serum was markedly increased to 509, 417, and 191 U/L, respectively (normal range 25-60 U/L). Lactate dehydrogenase (LD; EC 1.1.1.27) was also increased, with LD-3 predominating. The increased concentrations of c-AP and LD presumably originated from the destruction of infected, activated lymphocytes, especially T lymphocytes. Necrotizing lymphadenitis is probably caused by a lymphocytotropic virus.

Aminopeptidases↗
PubMed1990

[Treatment of vegetative status with dorsum column stimulation].

UNLABELLED: We have already reported that DCS (Dorsum Column Stimulation) alleviates the clinical symptoms of patients in vegetative status. Since then, we have accumulated information about 23 cases that have been treated by DCS therapy. In this paper, we describe the clinical outcome and compare cases where the treatment was effective with those where it was non-effective to evaluate the indications for further use of this treatment. RESULTS: 1) Eight of these cases showed good clinical improvement. 2) Most of the cases where the treatment was effective were young. Starting neurostimulation early brought about better clinical effects. 3) The clinical effects appeared 1-12 months after the start of the neurostimulation. 4) CT findings in the cases which had been treated effectively showed no marked cerebral atrophy, and no bilateral large diffuse low-density areas not involving the thalamus. Neither did they show any definite and large low-density areas in the brainstem. In conclusion, when the dorsum column was stimulated to treat vegetative status in which CT disclosed none of the abnormal findings described above, it increased r-CBF, enhanced the metabolism of catecholamine, and brought clinical improvement.

Adolescent↗
PubMed1990

[A successful treatment of spontaneous carotid cavernous fistula causing hemorrhagic infarction treated by detachable balloon].

A rare case of hemorrhagic infarction associated with carotid-cavernous fistula is reported. The patient was a 74-year-old female. CT scan showed hemorrhagic infarction of the left superior temporal gyrus, irregular vascular enhancement of the bilateral front-temporal lobe, and dilatation of the cavernous sinus. Left carotid angiogram revealed a high-flow left-sided CCF, cross filling to the right cavernous sinus, and intracavernous aneurysm. Bilateral front-temporal cortical veins were visualized early in the arterial phase. In this case, there was a direct shunt between the internal carotid artery and the cavernous sinus, and venous hypertension. Therefore urgent treatment using detachable balloon catheters was performed. Postoperative digital subtraction angiography revealed the disappearance of CCF. In conclusion, in CCF with cortical venous drainage there is a high possibility of developing hemorrhagic infarction. Only immediate detachable balloon occlusion can improve the outcome in elderly patients.

Aged↗
PubMed1989

Laboratory and clinical features of lactate dehydrogenase subunit deficiencies.

Heterozygous subjects with lactate dehydrogenase (LD) subunit deficiencies do not always show a low serum LD activity. Thus, it is necessary to examine the serum and erythrocyte LD isozyme patterns when screening for these conditions. We assessed patients with heterozygotes for H and M subunit deficiency by immunoblotting with anti-H subunit antibody. We could not find any protein or variant proteins in the heterozygous subjects with M subunit deficiency. On the other hand, the existence of variant protein was confirmed in the heterozygous subjects with H subunit deficiency. However, in one patient with homozygous for H subunit deficiency, no H subunit protein was observed. It is suggested that possibly many types of LD subunit deficiency may exist. Only patients with homozygous for M subunit deficiency show common patterns of clinical findings. All patients with LD subunit deficiencies have the risk of misdiagnosis of other clinical conditions from laboratory data because LD release is reduced. Thus it is important to detect them as a latent hereditary symptom poor disorders recognizing in laboratory medicine.

Cross-Sectional Studies↗
PubMed1989

A long-term follow-up study of total hip replacement with bone graft. Correlations between roentgenographic measurement and hip mobility.

Fifty total hip replacement treating the acetabular deficiency with autologous bone graft from the femoral head were performed on 44 patients with congenital dislocation of the hip. The average age was 55 years. The average follow-up period was 5 years and 2 months, ranging from 3 years, 8 months to 6 years, 11 months. Solid union was demonstrated in all acetabular grafting. No loosening of either acetabular or femoral components occurred. Roentgenological measurements used as a parameter of good results showed that better results were obtained using the true acetabulum (ideal position) with appropriate bony coverage.

Acetabulum↗
PubMed1989

Implications of hyperechoic lesions in small hepatocellular carcinoma.

Of 34 solitary small hepatocellular carcinomas (HCC) 2 cm in diameter or less, 13 with hyperechoic lesions were observed serially by sonography, and 11 of these were examined histologically. Serial examination showed that hypoechoic areas appeared at the periphery of or within, the hyperechoic tumor, and that these areas expanded more with tumor growth than the hyperechoic areas as if compressing or displacing the existing hyperechoic areas. Histologically, the hyperechoic lesions were composed mostly of well-differentiated cancer cells containing fat droplets, whereas the hypoechoic lesions were composed of cancer cells without fat droplets. In the two tumors that were formed almost completely of cancer cells showing fatty metamorphosis, cancer cells without fat droplets proliferated mainly in the periphery of the tumor. These findings suggest that, in hyperechoic HCC, cancer cells with fat droplets appear in the early stage of HCC, and probably change into concer cells without fat droplets by the time that a certain tumor size is reached, with gradual displacement by the latter type of cell during tumor growth.

Biopsy↗
PubMed1989

A pitfall in the interhemispheric translamina terminalis approach for the removal of a craniopharyngioma. Significance of preserving draining veins. Part I. Clinical study.

The interhemispheric translamina terminalis approach has made total removal of a craniopharyngioma less risky. Nevertheless, one of the pitfalls of this approach is cutting of the draining veins combined with retraction. A case of craniopharyngioma in which a subcortical hematoma developed is reported. The divided draining veins and the 60-minute retraction period were thought to have caused this complication. We therefore determined the clinical effect of cutting the draining veins and retraction on the regional cerebral blood flow and the motor nerve action potential. There were marked changes and a hematoma occurred mostly when the veins were divided and retraction was applied over the area.

Action Potentials↗
PubMed1989

A pitfall in the interhemispheric translamina terminalis approach for the removal of a craniopharyngioma. Significance of preserving draining veins. Part II. Experimental study.

The authors have reported a case of a craniopharyngioma removed by the interhemispheric translamina terminalis approach, and the postoperative development of a subcortical hematoma. We suspected that the cause of the subcortical hematoma was dividing the bridging veins and applying a retractor over them. In this paper, we report our experimental study, using dogs and monkeys, to evaluate the effect of cutting the vein and applying a retractor over it. The incidence of a subcortical hematoma was highest (60%) when the veins were cut and the retraction was applied. Vein occlusion only did not cause hematoma to develop, and the retraction alone caused 13% of the hematomas. This clearly indicated that the clinical case reported in Part I developed the subcortical hematoma postoperatively because the frontal bridging vein was cut and a retractor was applied for 60 minutes over the cut vein.

Action Potentials↗
PubMed1989

Effects of dorsal column spinal cord stimulation (DCS) on reversibility of neuronal function--experience of treatment for vegetative states.

We have reported that DCS (dorsal column stimulation) improves the clinical symptoms of cases in persistent vegetative states. Since then, we have accumulated 23 cases who have been treated by DCS. We describe the clinical EEG, rCBF, and biochemical changes in cerebrospinal fluid (CSF) caused by DCS in patients in persistent vegetative states: (1) eight of these cases showed good clinical improvement; (2) most patients were young and the CT scan did not show large, diffuse, definite or bilateral low density areas in cortex, brainstem of thalmus; (3) neurostimulation increased rCBF (cerebral blood flow) in many parts of the brain in cases with vegetative states; blood flow increased in the cerebrum, cerebellum and brainstem; (4) neurostimulation enhanced the metabolism of catecholamines in CSF, NE, DA, DOPAC, HVA, and 5HIAA increased, but 3MT and 5HT decreased in CSF. DCS increased rCBF, enhanced the metabolism of catecholamines in CSF, and improved the EEG in patients in persistent vegetative states.

Cerebrovascular Circulation↗