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Biomedical subjects

T Kamio

Publications and source records attributed to T Kamio.

90 records · Page 5Linked to original sources
PubMed1981

Direct immunofluorescent staining of Leucocytozoon caulleryi of different developmental stages.

Direct immunofluorescence was first used successfully for the staining of Leucocytozoon caulleryi of different developmental stages, such as the 2nd generation schizont and merozoite, gametocyte, zygote, ookinete, and sporozoite. Fluorescent antibody of IgG nature prepared from sera of chickens over a period from the 21st to the 100th day after sporozoite inoculation was used in this experiment. The cross reactivity on different developmental stages of L. caulleryi in the direct fluorescent antibody test suggested that antigens common to different developmental stages, or shared antigens, might exist, as well as specific antigens so far well-known. The fluorescent intensity of oocyst sporozoite, and schizont contents was apparently lower than that of any other stage. This evidence might be elucidated by the antigenic incompletion.

Animals↗
PubMedDate not supplied

Eustachian tube lymphatics.

Serous otitis media is the most common cause of hearing impairment. The role of lymphatic obstruction in the pathogeneis of serous otitis media is significant. A method for removeal of the human Eustachian tube specimen and two techniques for identification of Eustachian tube lymphatic capillaries are described. One involves the antemorten intratympanic installation of Berlin blue. The other utilizes electron microscopy. Lymphatic capillaries cannot be reliably differentiated from blood capillaries with the light microscope. With electron microscopy, lymphatic capillaries can be differentiated from blood capillaries by differences in the basement membrane. The lymphatic capillary has gaps in the basement membrane with large nuclei in the wall. A blood capillary has a continuous basement membrane and sometimes red blood cells be be identified in the lumen. Using these methods, Eustachian tube lymphatic capillaries in the human are described for the first time in this report.

Adolescent↗
PubMedDate not supplied

New neurotological test for detecting cerebellar dysfunction. Vestibulo-ocular reflex changes with horizontal vision-reversal prisms.

Adaptation of the vestibulo-ocular reflex (VOR) was studied in 26 normal subjects and 15 patients with cerebellar lesions, using horizontal vision-reversal prisms. In normal subjects, adaptation of gain after wearing prisms for one hour was approximately 50% of the VOR value in the dark. In contrast to this, patients with cerebellar lesions showed less adaptation, approximately 20% after a one-hour forced adaptation task. These cases showed three different types of abnormalities: 1) high gain before wearing prisms and normal adaptation, 2) high gain before wearing prisms and reduction of adaptation, 3) normal gain and reduction of adaptation. From these results it is suggested that observation of the effect of vision-reversal prisms on the VOR may permit the detection of cerebellar lesions of a type or subtlety which escape established tests.

Adult↗