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Biomedical subjects

T Kajiwara

Publications and source records attributed to T Kajiwara.

At least 145 records · Page 8Linked to original sources
PubMed1989

Effects of NSAID on collagen and proteoglycan synthesis of cultured chondrocytes.

Three major NSAID (tiaprofenic acid, indomethacin and acetylsalicylic acid) were studied with regard to their effects on the biosynthesis and gene expression of collagen and proteoglycan of chondrocytes. We found that the biosynthesis of type 11 collagen was suppressed in 60-70% of the controls by either indomethacin or acetylsalicylic acid; tiaprofenic acid did not suppress collagen synthesis. Although the biosynthesis of proteoglycan was suppressed to 80-90% of the control by indomethacin or acetylsalicylic acid, tiaprofenic acid did not suppress proteoglycan synthesis. In order to investigate the gene expression of type 11 collagen and proteoglycan, dot blot hybridization was performed using type 11 collagen and proteoglycan cDNA. The effects of NSAID on the amount of type 11 collagen and proteoglycan mRNA were consistent with those on collagen and proteoglycan biosynthesis. Thus, we concluded that tiaprofenic acid has less effect on cultured chondrocytes.

Animals↗
PubMed1988

Variations in the disappearance rate of indocyanine green.

Time-associated changes in the disappearance rate of indocyanine green from the blood (K.ICG) as an index of liver function, were studied. Blood was drawn 5 times at 3-minute intervals from 32 patients. Early, intermediate, and late K.ICG values were 0.087 +/- 0.040, 0.082 +/- 0.038, and 0.076 +/- 0.033 min-1, respectively, showing serial decreases. When blood was drawn 8 times at 2-minute intervals from 22 other patients, the means of the K.ICG values at 11 time points showed a nearly linear relationship (r = -0.986). These findings indicated that K.ICG is approximated by a linear function of time, K(t) = -K'.t + K0. According to this function, K.ICG is considered to decrease by 1.96% every minute. The K.ICG value determined by the conventional method is, therefore, a mean disappearance rate of 15 minutes, and K0 is considered to reflect the initial reaction speed.

Cholestasis↗
PubMed1988

[Rational surgery of early gastric cancer].

Reduced operation for gastric cancer (R1 operation) is indicated for early gastric cancer without lymph node metastasis. We examined which cases can be regarded as early gastric cancer. Mucosal cancer of macroscopically I, IIa, and IIb type that is 2 cm or less in diameter and located at C and macroscopically IIa type submucosal cancer 1 cm or less in diameter appeared to be indicated for this operation. However, preoperative selection of these cases is difficult, and at present, reduction surgery cannot be performed with confidence. Therefore, we have proposed rational surgery for early gastric cancer consisting of removal of the tumor and dissection of group 1 lymph nodes, the lymph node along the left gastric artery, that along the common hepatic artery, and that around the celiac artery. This procedure seems to be appropriate in terms of antitumor immune response of regional lymph nodes and surgical results.

Humans↗
PubMed1988

[Cerebral aspergillosis as a cerebral vascular accident].

Cerebral aspergillosis is one of the most common mycotic infections in the central nervous system causing different clinical features such as brain abscess, granuloma, meningitis, and encephalitis. Cerebral aspergillosis, however, may lead to a cerebral vascular accident such as intracranial hemorrhage or cerebral infarction. In this report, we present two patients with cerebral aspergillosis accompanied by intracranial hemorrhage. A total of 124 reported cases of cerebral aspergillosis are reviewed to ascertain the pathogenesis of the associated vascular lesion. The first patient was a 9-year-old girl, who developed drowsiness with a headache during the medical treatment for acute myelocytic leukemia. CT disclosed subarachnoid and intraventricular hemorrhage. The autopsy revealed that the aspergillus arteritis was the cause of repeated hemorrhage. The second patient was a 15-year-old boy with allergic purpura and renal failure, who suddenly developed a stupor with convulsive seizure. CT disclosed an intracerebral hemorrhage in the right parieto-occipital area. The patient gradually deteriorated and died in spite of the surgical removal of the hematoma. The autopsy revealed that the hemorrhage was caused by the aspergillus arteritis. Cerebral aspergillosis has two routes of infection to the central nervous system: hematogenous dissemination from the distant site (usually the lung) and direct extension from the contiguous site (usually the paranasal sinuses or orbit). The primary mechanism of neuropathology is different between these two types. Primary cerebral arteritis is most often seen in patients with the former type, whereas primary basal meningitis occurs in the latter. The incidence of clinico-pathological features is different between hematogenous dissemination type and direct extension type.(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗
PubMed1987

Exocrine and endocrine stomach after gastrobulbar preserving pancreatoduodenectomy.

Exocrine and endocrine stomach was studied serially in 13 patients who had gastrobulbar preserving pancreatoduodenectomy (GPPD). In most of them, acid output temporarily increased just after operation but recovered. Gastrin response level decreased transiently but returned to the preoperative level. A negative correlation was observed between the acid and gastrin levels, which suggests that the negative feedback mechanism between parietal cells and G cells was maintained. Acid and gastrin levels in GPPD were higher than those in conventional pancreatoduodenectomy (cPD) but not remarkably different from those of the controls. No peptic ulcer was detected after the operation. These findings indicated that GPPD poses little problem concerning offensive factors. Postoperative ulcer formation is considered to be prevented by the authors' procedure, which is devised to best preserve defensive mechanisms so that duodenectomy is minimized and the gastrointestinal continuity is reconstructed physiologically from mouth to anus by end-to-end duodenoduodenestomy, end-to-side pancreatojejunostomy, and end-to-side choledochojejunostomy.

Adult↗
PubMed1987

[Clinical evaluation of tumor markers in patients with colorectal cancer].

In 117 patients with colorectal cancer, the serum levels of CEA, TPA, CA 19-9, IAP, and, AFP have been compared with the clinical findings. The combined assay of 5 tumor markers were found to be useful for the screening of colorectal cancer. Excluding the AFP, positive rate of the markers for colorectal cancer was higher than that for breast cancer (p less than 0.01) and similar to that for gastric cancer. The serum levels of TPA, CEA and CA 19-9 correlated with the histological progression. Thus, they may be an indicator of the degree of lymph node metastasis, liver metastasis, the histological depth, and also may be useful in evaluating the prognosis of patients with colorectal cancer.

Antigens, Neoplasm↗
PubMed1986

Factor VIII concentrate-responsive thrombocytopenia, hemolytic anemia, and nephropathy. Evidence that factor VIII:von Willebrand factor is involved in its pathogenesis.

A 4-year-old Japanese girl had a congenital disorder that was characterized by recurrent thrombocytopenia, hemolytic anemia, hematuria, and proteinuria, which were repeatedly improved by the infusion of factor VIII concentrate. She developed the similar symptoms within 1 h after 1-desamino-8-D-arginine vasopressin (DDAVP) administration. Coagulation studies 30 and 60 min after DDAVP infusion showed a disappearance of large factor VIII:von Willebrand factor (VIII:vWF) multimers, which was the same abnormality that was observed at acute episodes. There were no significant changes in the plasma levels of 6-keto-prostaglandin F1 alpha and thromboxane B2 before and after DDAVP infusion. These results provide further support that VIII:vWF is directly involved in the pathogenesis of this congenital disorder.

Anemia, Hemolytic↗
PubMed1985

[Clinical utility of tumor markers in gastric and colon cancer].

Serum TPA and CEA level were measured in patients with gastric or colon cancer during the course of surgical treatment, and those clinical utility was evaluated. Both markers level were shown to be highest in colon cancer, followed by gastric cancer, benign diseases, and healthy subjects. Late stage patients, especially inoperable patients or patients in recurrence showed significantly high TPA and CEA level compared to early stage patients. Both markers were considered to be useful not only postoperative following up for recurrence but also preoperative evaluation for resectability and prognosis and postoperative evaluation for the treatment itself.

Antigens, Neoplasm↗
PubMed1982

Effects of the ionophore monensin on type II collagen and proteoglycan synthesis and secretion by cultured chondrocytes.

Detailed studies of the effects of the ionophore monensin upon avian chondrocyte ultrastructure, macromolecular synthesis, and macromolecular secretion have been carried out. Embryonic avian chondrocytes in suspension culture were incubated in concentrations of monensin ranging from 1 X 10(-7) to 1 X 10(-6) M for durations up to 8 h. Electron microscopy revealed that the treated chondrocytes developed abnormal Golgi structures and a markedly distended rough endoplasmic reticulum. Biochemical and immunoassay studies showed that while total protein synthesis was only slightly impaired by monensin, the ionophore had pronounced effects upon the secretion of both type II collagen and proteoglycans. These two macromolecules responded to monensin inhibition in a similar fashion and accumulated within the affected chondrocytes. The kinetics of response over the monensin concentration range used was virtually identical for type II collagen and proteoglycan. Undersulfation of proteoglycan, caused by monensin, was examined by ion exchange chromatography and analysis of the products of chondroitinase ABC digestion. The results indicated that undersulfation affected all glycosaminoglycan chains in a general fashion rather than affecting a specific population of chains.

Animals↗
PubMed1982

Proteoglycan core protein is accumulated in cultured chondrocytes in the presence of the ionophore monensin.

The nature of the proteoglycan antigen which accumulates in chick embryo chondrocytes that had been incubated in monensin was examined. Both the culture media and cell lysates were immunoprecipitated using antibody which primarily is directed against core protein. Gel filtration and electrophoresis of the immunoprecipitates showed that molecules corresponding to completed proteoglycan, core protein, and link protein were present in the immunoprecipitates. Monensin caused the cellular accumulation of core protein which was both underglycosylated and undersulfated. These results suggest that monensin affects early events of proteoglycan biosynthesis and that it may be useful for elucidating those events.

Animals↗