Search PubMed⌕ Search

Biomedical subjects

T Kaizu

Publications and source records attributed to T Kaizu.

49 records · Page 3Linked to original sources
PubMed1976

The effects of aldosterone and spironolactone on renal kallikrein in the rat.

1. A technique has been developed for the measurement of kallikrein 'production' in rat renal cortical cells in suspension. 2. After preparative steps, column chromatography on DEAE-cellulose yielded a peak of alpha-N-tosyl-L-arginine methyl ester (Tos-Arg-OMe) esterase activity identical with kallikrein isolated from rat urine in respect of pH optimum, effects of inhibitors, biological activity and immunological properties. 3. The nutrient medium surrounding incubated cells contained measurable kallikrein activity, which was increased by aldosterone and decreased by spironolactone. 4. The results raised the possibility that kallikrein could be an aldosterone-induced protein.

Aldosterone↗
PubMed1975

Studies on rat renal cortical cell kallikrein. I. Separation and measurement.

A technique has been developed to separate and measure kallikrein in a heterogeneous population of rat renal cortical cells in suspension. After rat kidneys were perfused in situ in anaesthetized rats, viable, counted cortical cell suspensions were obtained. Cells were suspended in a sucrose/Tris buffer containing 0.5% deoxycholate, homogenized, centrifuged, dialyzed, and gel filtered on Sephadex G-25. Column chromatography on DEAE-cellulose resulted in a single peak of esterase activity between 0.20 to 0.25 M NaCl/sodium phosphate buffer. Subsequent elution yielded an alkaline esterase which was identical to kallikrein isolated from rat urine, insofar as pH optimum, effects of inhibitors, bioassay activity and immunological properties were concerned. Calculated yields were about 70% of the total esterase activity present in the parent cell homogenates. Recoveries of a purified rat urinary kallikrein added to the cell homogenates, the DEAE-cellulose columns, or the eluates from the columns ranged from 83-108% (mean 96%). Using this technique, it was found that the amount of kallikrein activity present in non-incubated renal cortical cells ranged from 0.6-10(-2) to 4.6 - 10(-2) alpha-N-tosyl-L-arginine methyl ester (Tos-Arg-OMe) esterase units per 10(8) cells. However, cells incubated in a nutrient medium at 37 degrees C for 3-8 h contained no measurable kallikrein activity, whereas the surrounding medium had kallikrein activity which could be significantly increased by aldosterone and decreased by spironolactone.

Aldosterone↗
PubMedDate not supplied

Radiation therapy for stage I and II testicular seminoma: changes in the treatment policy for the prescribed dose.

BACKGROUND: We set out to review treatment policy, survival rate and acute and late sequelae of histologically confirmed seminoma patients who underwent orchiectomy and radiation therapy. MATERIALS AND METHODS: Thirty patients with testicular seminoma were investigated. The follow-up ranged from 1-20 years, with a median of 8.6 years. There were 23 patients with Stage I, six with Stage IIA and one with Stage IIB of the disease. All patients were treated with orchiectomy followed by iliac and paraaortic irradiation (median dose: 3900cGy for Stage I and 4000 cGy for Stage II patients). Up to 1986 the median total dose was 40Gy, and this dose was administered to sixteen patients. After that, fourteen patients received a total dose of 32Gy. RESULTS: For patients with Stage I and Stage II, 5- year disease-free survival rate was 100%. Twenty-three patients experienced certain acute reactions or side effects and all became well after radiation therapy; however, almost no patients showed severe late complications. CONCLUSION: In our study, radical orchiectomy and postoperative irradiation of iliac and paraaortic lymphaticus give good results for patients with Stage I and II testicular seminoma.

Adult↗