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Biomedical subjects

T K Dzagurova

Publications and source records attributed to T K Dzagurova.

At least 37 records · Page 2Linked to original sources
PubMedDate not supplied

[Hantaan virus serotypes circulating in foci of the Far Eastern region of the USSR].

Studies on serodifferentiation by indirect immunofluorescence antibody method and neutralization test of Hantaan virus strains isolated from human patients and rodents revealed circulation on the Maritime Territory of 5 virus serotypes and demonstrated that rodents of one species could be virus carriers of different virus serotypes. On the basis of the antigenic similarity of strains isolated from human patients, field mice, and Clethrionomys rufocanus it is concluded that the virus serotypes circulating among rodents of these species may play a role in the etiology of cases of hemorrhagic fever with renal syndrome.

Animals↗
PubMedDate not supplied

[Virus-specific proteins and RNA of the virus of hemorrhagic fever with renal syndrome].

Virus-specific proteins G1, G2, and N with molecular weights of 70, 55-57, and 50 kilodaltons, respectively, were detected by radioimmunodiffusion tests in VERO E-6 cells infected with strains of virus of hemorrhagic fever with renal syndrome (HFRS) isolated in the European USSR from a patient with HFRS, a fatal human case of HFRS (the strains K-27 and P-360) and from a bank vole (strain CG-1820). The sera from human convalescents after HFRS in the European USSR and rat sera prepared with the CG-1820 strain precipitated proteins possessing similar electrophoretic characteristics from a lysate of cells infected with the CG-1820, K-27 and P-360 strains. The sera from human HFRS convalescents in the Far East did not precipitate protein Gl. The viral RNA derived by immunosorption method from intracellular nucleocapsids of CG-1820 strain and strain 4590 (isolated from Ap. Peninsulae in the Far East) contained 3 classes of molecules: L, M, and S. L- and M-RNA of these strains had the same molecular weight (1.62 and 1.38 megadaltons). The molecular weight of S-RNA of the strain 4590 was 0.76 megadalton and that of the CG-1820 strain 0.83 megadalton. It is assumed that there are species differences among the viruses, causative agents of HFRS, circulating in the European and Far East regions of the USSR.

Animals↗
PubMedDate not supplied

[Hemorrhagic fever with renal syndrome in Penza Province].

The evidence of the existence of natural foci of HFRS in Penza region has first been presented on the basis of serological examinations of patients and normal population as well as HFRS virus antigen demonstration in the lung tissue of small mammals. An outbreak of HFRS among children in a pioneer camp is described. Aerogenic infection of the children contracted from HFRS virus-infected bank voles (Clethrionomys glareolus) living near the camp is assumed. The detection of HFRS antigen in 5 species of small mammals indicates the polyhostal nature of HFRS foci existing in the region.

Animals↗
PubMedDate not supplied

[Detection of natural foci of hemorrhagic fever with renal syndrome on the territory of the Komi ASSR].

Natural foci of hemorrhagic fever with the renal syndrome (HFRS) have been found for the first time in the Komi ASSR. The main source of the causative agent in this territory was Clethrionomys glareolus the average infection rate of which was 4.4% and in some areas as high as 22.6%. For the first time in the republic the portion of human population naturally immune to HFRS was determined, comprising 5.2% of the population. The immune structure of the population did not differ from that in other focal territories of the European part of the USSR.

Adolescent↗
PubMedDate not supplied

[Detection of natural foci of the hemorrhagic fever with renal syndrome in the Georgian SSR].

The circulation of the causative agent of hemorrhagic fever with renal syndrome (HFRS) in Georgia was studied. Altogether, 7,938 small mammals of 16 species trapped in 23 administrative areas of the republic were examined, and the specific antigen of HFRS virus was detected in 308 (3.8%). In serological examinations of 10 patients, antibody to HFRS virus was found in 4. Analysis of the above results indicates the existence of natural foci of HFRS in the Georgian SSR.

Animals↗
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[Effectiveness of using cultured antigens for the serodiagnosis of hemorrhagic fever with renal syndrome by the immunofluorescence method].

Clinical serological studies of hemorrhagic fever with renal syndrome (HFRS) demonstrated high effectiveness of indirect immunofluorescence procedure (IF) for serodiagnosis of this infection. The use of HFRS virus-infected VERO-E6 cells as the antigen for IF enhanced the sensitivity and specificity of the method as compared with the use of cryostatic sections of the lung tissue from wild rodents spontaneously infected with HFRS virus as the antigen. A technology for preparation of a polyvalent HFRS diagnosticum containing the antigens of two virus serotypes has been developed for serological diagnosis of HFRS in the European foci where the serological evidence of circulation of strains of serotypes 1 and 2 of HFRS virus pathogenic for man has been obtained. The specificity and sensitivity of detection of antibody to HFRS virus serotypes 1 and 2 by IF test using the polyvalent diagnosticum is equal to that in tests using individually tissue culture antigens of HFRS virus serotypes 1 and 2. It was found that in the patients' native sera antibodies of the IgM class are masked by antibodies of the IgG fraction, therefore the use of IF technique for detection of IgM antibody to HFRS virus aimed at early serological diagnosis of this infection is not effective.

Animals↗
PubMedDate not supplied

[Stratum of the population of the Bashkir ASSR with natural immunity to hemorrhagic fever with renal syndrome].

Natural immunity to hemorrhagic fever with the renal syndrome (HFRS) in the human population of the Bashkir ASSR having the highest incidence of this infection was first studied using radioimmunoassay. The antigen was a suspension of lungs of rodents from natural foci containing high antigen titres. When 1726 human sera from 8 districts and 2 towns were examined, antibodies to HFRS were found in 20.1%, with variations from 6.9% to 41.5% in different territories. No relationship between the percentage of immune subjects and average incidence values for 17 years was found. In the immune population, men accounted for 21.3%, women for 19.3%. Among subjects under 40, the immune stratum was lower (17.5%) than among older subjects (26.2%). The percentage of immunity was found to depend on the occupation of the population. No significant difference in immunity could be found in persons with different blood groups. Antibody in HFRS convalescent was shown to persist in high titres for up to 20 years after the onset of the disease.

ABO Blood-Group System↗
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[Serologic examination of patients with hemorrhagic fever with renal syndrome in the European part of the USSR].

Certain features of humoral immunity in patients suffering from hemorrhagic fever with renal syndrome (HFRS) in the territory of the European USSR were established: a trend for increasing antibody levels for a long time after convalescence (approximately 6 months) and the lack of significant decline of antibody levels for many years (5-10). Comparative clinico-serological studies revealed the occurrence of subclinical and atypical forms of HFRS disease.

Adolescent↗
PubMedDate not supplied

[Indirect hemagglutination test for indicating the HFRS virus and the laboratory diagnosis of the disease].

Conditions have first been developed for the preparation of erythrocyte immunoglobulin diagnostic preparation of erythrocyte immunoglobulin diagnostic preparation for HFRS virus which permits express detection of the specific virus antigen in suspensions of the lung tissue of rodents by means of the indirect hemagglutination test (IHA) and laboratory diagnosis of the infection in humans by means of antibody detection by the indirect hemagglutination-inhibition test (IHAI). The IHA test is as sensitive as immunofluorescence, while the sensitivity of the IHAI test is not inferior to that of immunofluorescence but is higher than that of RIA.

Animals↗
PubMedDate not supplied

[Isolation of strains of the virus of hemorrhagic fever with renal syndrome in cell culture].

Four strains of hemorrhagic fever with renal syndrome (HFRS) virus were isolated from the lungs of Cl. glareolus caught in natural foci of HFRS in the European USSR. Preliminary experiments using enzyme-immunoassay (EIA) established the appurtenance of HFRS antigen in lung suspensions to the "western" serotype. Four strains were isolated in Vero-E6 cell culture after several blind passages and identified as the "western" serotype of HFRS virus. One stram originally isolated in Wistar rats and passaged in them 4 times and then adapted to Vero-E6 cells showed a closer antigenic relationship with the "eastern" HFRS virus serotype.

Animals↗
PubMedDate not supplied

[Genetic differentiation of hantaviruses using the polymerase chain reaction and sequencing].

Thirty-two hantavirus strains and 8 samples of lung tissue from rodents collected in different regions of Russia have been examined by molecular biological methods. Two methodological approaches have been employed for the study of genetic relationships between the viruses: nested PCR assay and common RT-PCR with subsequent direct sequencing of 200 and 365 base pair of G2 protein encoding regions of M-segment, respectively, and the resultant sequences were compared with those of the prototype hantavirus. The study revealed a mosaic pattern of distribution of different hantavirus genotypes on the territory of Russia.

Animals↗
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[Dot-blot analysis in laboratory diagnosis of hemorrhagic fever with renal syndrome].

A sensitive micro EIA utilizing antigen or antibody dotted onto nitrocellulose filters (Dot-ELISA, Dot-blot) has been developed for laboratory diagnosis of hemorrhagic fever with the renal syndrome (HFRS) by detecting the virus specific IgM and IgG in the sera of HFRS patients and the antigen in crude lung suspensions of wild rodents. Measurements of specific IgM and IgG in paired HFRS sera collected during the first month of the disease showed clear-cut seroconversion and 100% correlation with the results of immunofluorescent test. The results of antigen detection in lung suspensions of 605 wild rodents trapped in various regions of Russia were identical to standard ELISA results. This rapid and inexpensive test may be useful in early serologic diagnosis of HFRS and in field serological and epizootological studies.

Animals↗
PubMedDate not supplied

[Polyclonal immunoenzyme system for determining hantaviral antigens: assessment of specificity using monoclonal antibodies and the polymerase chain reaction].

Genus-specific monoclonal antibodies to nucleocapsid protein of Pumala virus and polymerase chain reaction were used to assess the specificity of a previously described polyclonal (based on human IgG to Pumala hantavirus) enzyme immunoassay (EIA) system for detecting hantavirus antigens. Parallel testing of crude lung suspensions from wild rodents trapped in different regions of Russia for hantavirus antigens and RNA showed 100% coincidence of the results of three tests. These data indicate a high specificity of polyclonal EIA system and its efficacy for screening of natural samples.

Animals↗
PubMedDate not supplied

[A system of immunoenzyme analysis using biotinylated monoclonal antibodies for typing hantavirus antigens].

Enzyme immunoassay with biotin-labeled monoclonal antibodies to Puumala virus was used for typing hantavirus antigens. The system is type-specific, that is, it detects Puumala virus only, which was demonstrated by typing 9 hantavirus antigens in infected Vero E5 cells and by epizootological analysis of an HFRS outbreak in the Yegoryevsk district of the Moscow region in November-December 1995. The suggested system may be used as an element of EIA screening of natural samples in analysis of the screening results.

Antibodies, Monoclonal↗