Biomedical subjects

T Juhás

Publications and source records attributed to T Juhás.

At least 19 recordsLinked to original sources

PubMed2004

[Immunofluorescent analysis of anterior lens capsule allotransplantation for chronic corneal ulcers].

This study uses immunofluorescent analysis to find out in histologic corneal sections a possible rejection reaction in anterior lens capsule allotransplantation for chronic corneal ulcers. The experiment included control group of rabbit corneas with repeat injury to epithelium and anterior stroma as well as surgically treated group. Fluorescein-conjugated human antibodies to IgA, IgM, IgG, complement and fibrinogen were used in the study. For detection we used method of direct immunofluorescence. Positive was only examination on fibrinogen in sections of treated group eyes 2 days after surgery. Last examination after 3 months failed to detect positivity in any of the tested markers. We preliminarily conclude that anterior lens capsule allotransplantation for chronic corneal ulcers does not result in rejection reaction and that components of humoral immunity do not participate in immune reaction.

Animals↗

PubMed2003

UV-B induced production of MMP-2 and MMP-9 in human corneal cells.

The purpose of this study was to determine the production of metalloproteinases (MMP) 2 and 9 following UV-B irradiation in human corneal epithelial cells and fibroblasts. Epithelial cells and fibroblasts were separated from human donor corneas and exposed to UV-B lamp irradiation for 20, 40, 80 and 120 s. Media samples were collected at 8, 24, 48 and 72 h and gelatinase A and B production was assayed by the ELISA test. Statistical significance of production was assessed by the paired t-test. Increased production of MMP-2 was found in human corneal fibroblasts in response to UV-B irradiation. A statistically significant production of MMP-2 was not observed in human corneal epithelial cells following UV-B exposure. We did not detect any increase in MMP-9 after irradiation in either epithelial cells or fibroblasts. MMP-2 is produced by the corneal fibroblasts in the acute phase after UV-B irradiation. MMP-9 is not released in vitro following UV-B irradiation damage and therefore does not directly participate in the pathophysiology of acute photokeratitis.

Cells, Cultured↗

PubMed2003

[Corneal topography and LASIK].

PURPOSE: The purpose of this study is to outline the importance of corneal topography after LASIK surgery and to show the association between topographic findings and postoperative results. PATIENTS: One hundred and fourteen eyes, 70 patients with myopia (from -6.00 to -14.00 Dsph) who underwent surgery by LASIK. METHODS: We identified 5 types of topographic patterns which were classified to symmetric and asymmetric groups. We evaluated the association between the type of topography pattern and the following parameters: age, sex, astigmatism, pre-operative refraction, post-operative uncorrected and best corrected visual acuity. RESULTS: Fifty eight eye filled the criteria of the first group (uniform pattern), 30 eyes of the second group (bowtie), 4 eyes of the third group (penninsula), 3 eyes of the fourth group (semicircular) and 3 eyes of the fifth group (irregular pattern). Significant association between topographic corneal patterns and observed parameters has been confirmed in post-operative uncorrected visual acuity, post-operative refraction and astigmatism (p < 0.05). Age and sex were not significant parameters for the outcome of surgery. CONCLUSION: Majority of eyes (89%) had symmetric corneal topography pattern which highly surpassed our expectations. Comparison of topography patterns with observed parameters proves that the shape of ablation zone influences post-operative quality of vision.

Adolescent↗

PubMed2003

[Immunofluorescence identification of limbal stem cells in a culture of donor corneas].

The purpose of this study was to identify limbal stem cells in the culture of human donor cornea. After standard cultivation procedure we used monoclonal antibody against alpha-enolase for identification of stem cells. This antibody serves as a probe for the identification of biochemical marker of stem cells. Using immunofluorescent microscopy we identified in specimens alpha-enolase positive cells which in vitro but not in situ showed also some morphologic differences from other cellular elements of the culture.

Antibodies, Monoclonal↗

PubMed2003

[Surgical correction of lagophthalmos by implantation of a gold weight].

This study concerns the problematics of upper branch of n. VII rehabilitation. Surgical technique is presented which involves implantation of gold weight into the upper eyelid which was used in 17 patients. The study emphasizes importance of oculoplastic surgery which should become an integral part of management of facial nerve paresis.

Adult↗

PubMed2001

[The histopathologic picture after chemical eye injury and the role of limbal stem cells].

The aim of this experimental study was to evaluate the role of limbal stem cells on corneal wound healing. The experimental objects were New Zealand white rabbits. Damage to the cornea was caused by 2.5 N NaOH solution for 50 sec. and was subsequently washed out by saline solution. The rabbits were euthanased 24 hours after alkaly burn and the tissue was fixated in 10% formalin. The samples were embedded in paraffin and stained with haematoxilin and eosin. The specimen showed focal inflammatory reaction with the presence of macrophages an round--nucleated cells in the limbal area.

Animals↗

PubMed2000

[The excimer laser and severe myopia--comparison of LASIK and PRK].

The aim of this study is to compare the results of photorefractive keratectomy (PRK) with method of laser-assisted keratomileusis (LASIK) in the treatment of high myopia by excimer laser. The study compares 100 eyes after PRK operated on by technique of multizonal ablation divided into group of moderately high myopia (-6.0 ... -9.0 Dsph/85 eyes) and severely high myopia (over -9.25 Dsph/15 eyes) with a group o 100 eyes operated on by LASIK similarly classified (moderately high myopia/67 eyes and severely high myopia/33 eyes). The eyes were treated by excimer laser Keracor 117 manufactured by Technolas company. Statistic analysis used one-way analysis of variance. One year after operation emmetropia within +/- 1.0 Dsph was 52% in the first and 47% in the second group after PRK. After LASIK it was 71% and 54% respectively. No statistically significant differences have been found between the groups. In spite of this, analysis of postoperative complications, line loss of the preoperative best spectacle corrected acuity and patients satistaction shows that LASIK is a better method in correction of high myopia by excimer laser.

Adult↗

PubMed1999

[Comparison of hyperopic photorefractive keratectomy and LASIK in correction of hypermetropia with excimer laser].

The aim of this work is to compare results of the correction of hyperopia by method of hyperopic photorefractive keratectomy (HPRK) with method of laser assisted keratomileusis known as LASIK. We evaluate group of 129 eyes operated by excimer laser Keracor 117 manufactured by Technolas company. 77 eyes underwent HPRK and 52 eyes hyperopic LASIK (HLASIK). The patients were divided to two groups, the first of which comprised 47 eyes with low hyperopia to +3.5 Dsph and the second 82 eyes with high hyperopia. Follow-up period was 12 months results were statistically evaluated by Student t-test. One year after treatment, in the first group was 62% eyes with hyperopia to +1.0 Dsph after HPRK and 70% after HLASIK. In the second group it was 32% and 44%, respectively. No statistically significant differences have been found between the two groups. In spite of that, we consider HLASIK method better than HPRK from the point of view of stability of results, occurrence of complications and estimated resulting refraction, especially in the eyes with low hyperopia.

Adult↗

PubMed1999

[Iris cysts].

Explore the source record for details and available documents.

Cysts↗

PubMed1998

[Implantation of silicone intraocular lenses--long-term results].

The author reports long time experience with implantation of silicone intraocular lenses. Briefly is described surgical technique, mainly the most common methods of folding of silicone intraocular lenses. Discussion concerns peroperative and postoperative complications and evaluation of the results after 3 years follow up.

Humans↗

PubMed1998

[Endonasal endoscopic dacryocystorhinostomy].

Contemporary advances in endoscopic surgery of the paranasal sinuses make it possible to identify and open the lacrimal system via the nasal sinus. The authors describe four cases of chronic dacryocystitis with obstruction of the nasolacrimal duct in three women and one man aged 22 to 56 years. In all four patients the lacrimal sac was opened endoscopically by the endonasal route and safeguarded by drainage. In the discussion the authors deal with contemporary views on this problem.

Adult↗

PubMed1997

[Argon laser trabeculoplasty--long-term results].

A group of 302 eyes in 175 patients with primary open-glaucoma (POAG) were analyzed after argon laser trabeculoplasty (ALT) with postoperative follow-up of 2 up to 10 years. ALT was considered to be successful when IOP remained under 21 mmHg with no further damage of the optic disc or visual fields. The success rate was in 81.8% eyes after two years, 64.4% after five years, 27.8% after 8 years of the follow-up, and only 15.6% eyes were still controlled after 10 years. Our results confirmed, that ALT was effective in the eyes with POAG, yet there was a substantial diminishing effect as time progressed.

Aged↗

PubMed1995

[Neodymium:YAG trabeculotomy].

The author presents the results of ND: YAG trabeculotomy on 10 eyes with open-angle glaucoma. The surgery has been carried out as last treatment before trabeculectomy. The mean intraocular pressure (IOP) before laser surgery was 35 +/- 5.2 mm Hg. The IOP decrease below 22.0 mm Hg has been achieved in 50% of eyes. The mean follow-up of the patients was 1 year. Complications included transient postoperative IOP elevation (six eyes) and angle bleeding (three eyes).

Adult↗

PubMed1994

[Diode laser trabeculoplasty in the treatment of primary open-angle glaucoma].

The results of diode laser trabeculoplasty carried out on 14 eyes of primary open-angle glaucoma are demonstrated. Powers of 0.8-1.0 W were used, with spot 75 um and a pulse of 0.1 second and a mean ocular hypotensive effect of 6.82 +/- 2.94 mmHg (27.9%) was observed at 6 months after treatment. The 78.6% compensation was achieved. The results confirm that diode laser trabeculoplasty is an effective mode of treatment for eyes with primary open-angle glaucoma.

Aged↗

PubMed1994

[Nd:YAG laser trabeculoplasty].

The results of ALT and Nd: YAG LTP are presented and compared in group 19 patients. The average IOP reduction and increase of outflow facilities were significant in both methods. Any difference of results between comparing methods were not statistically significant.

Female↗

PubMed1994

[Neodymium-YAG laser capsulotomy].

The results of 79 Nd: YAG capsulotomies in patients after extracapsular cataracta extraction are presented. The improvement in visual acuity of one or more Snellen lines in 94.1% eyes was observed. The complications included transitory hypertension more than 40 mmHg in 1.4% eyes, damage to the intraocular lens in 40.3% eyes, disruption of the anterior hyaloid face with forward displacement of vitreous into capsulotomy din 38.8% eyes. The others complications like retinal detachment, corneal damage, hemorrhage or macular edema were not observed.

Cataract Extraction↗

PubMed1993

[The myopic intraocular lens in a phakic eye. Initial experience].

The very first experiences with implantation of 3 phakic anterior chamber lenses for high myopia correction are presented. After average 4 month follow up good stability and transparation of concave lenses type ZB5M were observed with accurately achieved correction postoperatively.

Adult↗

PubMed1992

[Isolation of Dirofilaria repens in vitreoretinal findings].

The authors present the case of a 18-year-old patient who reported deteriorated vision and mobile dark shadows on the left eye. Examination revealed in the vitreous body an extremely mobile filiform, worm-like formation, white in colour, of the size of an arteriole, about 9 mm long. In the course of hospitalization the formation was isolated by surgery of the pars plana vitrectomy and subjected to detailed helminthological examination. The worm was identified as Dirofilaria repens (Reillet et Henry 1911) and described in detail. The presented finding is the first finding of this parasite in this country and at the same time the first finding of this parasite in Czechoslovakia.

Adolescent↗