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T J Tong

Publications and source records attributed to T J Tong.

12 recordsLinked to original sources
PubMed1994

[Cyclins].

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Animals↗
PubMed1993

[Study on endocytosis and nuclear accumulation of epidermal growth factor (EGF) in normal C3H mouse fibroblast cells C3H10 T1/2C18 and 3H-TdR transformed counterpart cells].

In this study, we investigated endocytosis and nuclear accumulation of epidermal growth factor (EGF) in normal C3H mouse embryo fibroblast cells C3H10 T1/2 C18 (designated NC3H10) and 3H-TdR transformed counterpart cells (designated TC3H10). The results revealed that 125I-labelled EGF bound to the receptor on the cell surface of the two cell lines was subsequently endocytosed and translocated from the cell membrane to the nucleus, where it progressively accumulated. NC3H10 showed more EGF accumulation in nucleus than TC3H10 (p < 0.05). SDS-polyacrylamide gel electrophoresis revealed that the endocytosed 125I-EGF associated with the nucleus was intact, with negligible breakdown products present. The nuclear accumulation of intact EGF in the mentioned cell lines exposed to ammonium chloride (a lysosomal inhibitor) was higher than the control (p < 0.05). These results suggested that receptor-mediated endocytosis and nuclear accumulation of EGF may play an important role in mediation some of the action of EGF.

Animals↗
PubMed1992

[The effect of epidermal growth factor on the expression of erb B2/neu oncogene in mouse embryo fibroblast cells].

The erb B2/neu oncogene encodes a protein which sequence is closely similar to the epidermal growth factor receptor (EGFR). We have previously found that EGF can induce the expression of erb B1/EGFR gene in normal and 3H-TdR transformed C3H/10T1/2CL8 mouse embryo fibroblast cells i.e. NC3H10 and TC 3H10 respectively, but we do not know whether the neu oncogene expression can be induced by EGF. In this study, the effect of EGF on NC3H10 and TC3H10 has been observed by Northern blot analysis. The result indicated that EGF had a obvious induction effect on neu oncogene expression in these cells. Thus, the expression of both erbB 1/EGFR gene and erbB 2/neu oncogene can be induced by EGF. This result may provide a novel clue to the molecular mechanism of EGF action in cell nucleus.

Animals↗