Search PubMed⌕ Search

Biomedical subjects

T J Rohner

Publications and source records attributed to T J Rohner.

At least 55 records · Page 3Linked to original sources
PubMed1978

Recognition of virally transformed cells by lymphocytes from patients with prostatic cancer.

Data presented describe the first assay using human peripheral blood lymphocytes (PBL) against two unique virally transformed cell lines in vitro. Human cells transformed by a cytomegalovirus (CMV-Mj) isolated from normal human prostate tissue were used as target cells in microcytoxicity assays with lymphocytes from 100 patients. Three target cell types were used: control human embryonic lung cells (HEL), transformed HEL cells (CMV-Mj-HEL-2), and transformed HEL cells retrieved from tumors induced in athymic nude mice (CMV-Mj-HEL-2, T-1) by injection of CMV-Mj-HEL-2 cells. PBL preparations from 84% of all patients tested significantly killed CMV-Mj-HEL-2, T-1 cells. However, only PBL from patients with prostatic carcinoma were cytotoxic for CMV-Mj-HEL-2 cells significantly more often than for control HEL. The implications of this approach are discussed.

Adult↗
PubMed1978

Establishment and characterization of a new human urinary bladder carcinoma cell line (PS-1).

An epithelioid cell line (PS-1) has been established from a transitional cell cancer derived from human urinary bladder. Subcutaneous injection of the epithelioid cells into weanling athymic nude mice induced solid tumors histologically similar to the original tumor. A cell line was also established from a tumor induced in the athymic nude mouse (PS-1, T-1). Both cell lines exhibited essentially identical growth characteristics and formed a monolayer growth of epithelioid cells in culture. Electron microscopic studies confirmed epithelioid morphology. No fibroblastoid elements were observed. Chromosomal analysis revealed heteroploidy with persistent marker chromosomes; all cells contained a Y chromosome. The presence of tumor-specific antigen(s) in PS-1 cells was suggested by microcytotoxicity assays with peripheral allogeneic lymphocytes from other transitional cancer cell patients. Sera of urinary bladder cancer patients reacted with nuclear antigens of the established cells.

Animals↗
PubMed1977

In vitro culture of human prostatic tissue.

A procedure is described which yields a significant percentage of long-term mixed cell cultures of human prostatic tissue. Attempts were made to suppress the proliferation of stromal fibroblasts and to characterize the cultured cells as those of prostatic origin. The problems associated with establishing epithelial cell lines are discussed.

Acid Phosphatase↗
PubMed1977

Clinical and radiographic findings of focally infected polycystic kidneys.

Three patients with localized polycystic kidney infections are presented with the pertinent clinical, laboratory, and radiographic findings. Gallium-67 citrate and angiography play an important role in evaluation of these patients. Angiography in particular is valuable in the diagnosis and the exact localization of the inflammatory disease. Localization is extremely important in planning surgical treatment should conservative therapy fail.

Adult↗
PubMed1977

Perinatal asphyxia and renal failure in neonatal patients.

Herein we discuss oliguria and azotemia in neonatal patients associated with perinatal complications, including difficult labor and delivery, and respiratory asphyxia. Renal failure in these patients is accompanied by proteinuria, microscopic hematuria and red blood cell casts, and it generally resolves in 7 to 10 days. Umbilical aortography can be helpful in determining the presence of normal kidneys in these patients.

Asphyxia Neonatorum↗
PubMed1977

Evidence for the association of cytomegalovirus with carcinoma of the prostate.

A human genital isolate of cytomegalovirus is shown to have transformed human embryonic lung cells in vitro. These cells produce tumors when injected into athymic nude mice. Two cell lines derived from tissue from human prostatic carcinoma have survived more than 20 passages in vitro and demonstrate cytomegalovirus-specific membrane antigen. Significant humoral antibody titers against cytomegalovirus have been demonstrated. Cell-mediated lymphocytotoxicity against these transformed cells has been demonstrated in patients with urinary tract tumors. This evidence indicates that an association between cytomegalovirus and human prostatic cancer may be more than coincidental.

Adenocarcinoma↗
PubMed1977

Lymphocyte reactivity against virally transformed cells in patients with urologic cancer.

Lymphocytes from patients with urologic cancer were tested in microcytotoxicity assays against human cells transformed by cytomegalovirus. Human lymphocytes were significantly cytotoxic against the transformed cell line when compared to a normal human control cell line. Patients with prostatic carcinoma demonstrated greater target cell reduction than those with benign prostatic hyperplasia.

Adenocarcinoma↗
PubMed1977

The effects of castration on adrenal testosterone secretion in men with prostatic carcinoma.

Selective adrenal vein catheterization was done on intact and castrated men with prostatic carcinoma. Adrenal to peripheral venous testosterone gradients were observed in all patients, indicating adrenal production of this hormone. No compensatory adrenal production of testosterone was noted during a 17-month period after orchiectomy. The data suggest that the human adrenal in castrates produces testosterone, which may explain why adrenal ablation can offer palliation in some patients with prostatic carcinoma.

Adrenal Glands↗
PubMed1976

Diagnosis of hyperparathyroidism.

Hyperparathyroidism is an important cause of calculous disease. A simple reliable protocol for evaluation of patients has been developed. In 20 patients with hyperparathyroidism this protocol has produced no false positive studies. Normocalcemic hyperparathyroidism can be diagnosed by this convenient method, and this protocol can be carried out in any hospital as part of an out- or inpatient workup.

Adult↗
PubMed1976

In vitro viral transformation of human prostatic carcinoma.

Cell cultures derived from fetal human and prostatic adenocarcinoma were inoculated with SV40 virus. Within 6 weeks in vitro morphologic transformation occurred, after which the cells proliferated rapidly. They exhibited the SV40 T-antigen by immunofluorescence and survived propagation beyond the limited lifespan of uninfected cultures. We suggest that this may be a useful technique in establishing stable cell lines of human prostate.

Adenocarcinoma↗
PubMed1976

Acute interstitial nephritis.

Acute allergic interstitial nephritis is manifested clinically by rash, fever, eosinophilia, hematuria, oliguria and azotemia. Histologically a monocytic inflammatory process in the renal interstitium is seen. The clinical course of a patient after excessive sodium cephalothin administration suggested allergic interstitial nephritis and implicates this drug as an etiologic agent.

Acute Disease↗
PubMed1976

Aminoglutethimide medical adrenalectomy for advanced prostatic carcinoma.

Complete adrenal suppression with aminoglutethimide has been accomplished in 7 patients with progressive stage D carcinoma of the prostate who had become refractory to orchiectomy and the administration of exogenous estrogens. A favorable response was noted in 3 patients. These preliminary results indicate that this agent may be as useful as surgical adrenalectomy in the treatment of progressive prostatic carcinoma. A brief discussion of the pharmacology of this agent, its mode of administration and the side effects are presented.

Adenocarcinoma↗
PubMed1976

Cystinuria.

Explore the source record for details and available documents.

Adult↗
PubMed1976

In vitro dog detrusor strip contractile responses and calcium ion activity.

In vitro detrusor strip experiments show that the presence of calcium ion is essential for spontaneous and bethanechol-induced detrusor contractile activity. 45Ca uptake experiments show that detrusor strips subjected to bethanechol show increased 45Ca uptake with significant decrease in total muscle strip calcium. There is a significant increase in the percentage of calcium exchange, suggesting that calcium efflux has exceeded influx. Kinetic analysis of 45Ca uptake by detrusor strips shows more 45Ca to reside in the slow component of strips following exposure to bethanechol. This suggests that calcium associated with bethanechol-induced contractile activity comes to occupy intracellular or more tightly bound membrane sites.

Animals↗
PubMed1975

Preliminary evaluation of urinary polyamines in the diagnosis of genitourinary tract malignancy.

Normal ranges for urinary polyamines have been obtained. Non-malignant genitourinary tract disease is not associated with elevated urinary polyamines. A high correlation exists between the presence of active urologic cancer and elevated urinary excretion of polyamines. Post-treatment polyamines correlate with the continued presence or absence of genitourinary tract malignancy.

Adenocarcinoma↗
PubMed1975

Imipramine toxicity.

Clinical experience has firmly established the usefulness of imipramine in the management of enuresis. Our report describes the serious dose-related cardiac and central nervous system toxic effects of imipramine. Comparison of the human and animal toxicity data is presented and recommendations are given for safe human dose ranges.

Animals↗