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T J Robinson

Publications and source records attributed to T J Robinson.

115 records · Page 7Linked to original sources
PubMedDate not supplied

Derivation and characterization of a somatic cell hybrid containing the portion of mouse chromosome 11 (MMU11) homologous to human chromosome 17q.

To contribute to the physical gene map of mouse chromosome 11 (MMU11) and to extend the mapping resources available for this chromosome, we have produced mouse x rat somatic cell hybrids containing only bands B5 to E of MMU11. Characterization of the hybrids by polymerase chain reaction (PCR) amplification and Southern blot analyses of MMU11 markers revealed two hybrids, T16Ad14B and T16Ad19A, that had selectively retained the 3(11) translocation product containing distal MMU11 (bands B5-E). Cytogenetic analysis of the hybrid T16Ad14B by fluorescence in situ hybridization (FISH) and conventional G-banding confirmed the presence of the 3(11) translocation chromosome. Mapping of markers in both the T16Ad14B and T16Ad19A hybrids localized the T16Ad translocation breakpoint between the proximal markers Atplb2 and Acrb and the more distal markers Scya2 and Mpo. Loci for D11Mit5, Rpo2-1, Trp53, Glut4, Acrb, and Atplb2 could all be localized proximal to the T16Ad breakpoint in band B5, between bands B1 and B5 on MMU11.

Animals↗
PubMedDate not supplied

Genetic divergence in South African Wildebeest: comparative cytogenetics and analysis of mitochondrial DNA.

The blue and the black wildebeest, Connochaetes taurinus and C. gnou, are currently classified as congeneric, but previous reports have placed C. taurinus in its own genus, Gorgon. To further clarify the evolutionary relationship between these two species, we examined and compared their mitotic chromosomes and mitochondrial DNA (mtDNA). No species-specific G-banded or C-banded chromosomal markers were found, and we conclude that the karyotypes are invariant at the level of resolution obtained. An evolutionary divergence time of approximately 1 million years was calculated from mtDNA restriction fragment data, indicating a close phylogenetic relationship for the two wildebeest species. The low nucleotide diversity detected within the black wildebeest (0.09%) is thought to reflect the recent population bottleneck to which the species has been subjected. In contrast, the limited heterogeneity (0.02%) within the South African blue wildebeest herds sampled in this study was surprising, and we argue that for many populations, especially those on smaller reserves, this may reflect common descent from a small number of animals through management-controlled translocations.

Animals↗
PubMedDate not supplied

Genetic divergence in South African wildebeest: analysis of allozyme variability.

Genetic variability within the blue (Connochaetes taurinus) and black (C. gnou) wildebeests in South Africa was estimated from allozyme frequencies at 31 protein-encoding loci. Seven loci, Ak-1, Alb, Est-D, Icdh-1, Mpi-1, Pgm-1, and Tf, were polymorphic in the blue wildebeest, but only two loci, Est-D and Tf, were variable in its congener. Average gene diversity was H = 0.018 +/- 0.013 in the black wildebeest and was significantly smaller than that, H = 0.081 +/- 0.030, in the blue wildebeest. The reduced level of heterozygosity in black wildebeest is most likely due to a bottleneck in population size in the early 1900s. The genetic distance between the two species was 0.059 +/- 0.027, which is consistent with an estimate of low mtDNA sequence divergence found previously.

Africa, Southern↗
PubMedDate not supplied

Assessing dyspepsia in general practice.

The value of the symptom pattern in achieving an accurate diagnosis in dyspeptic disease is still controversial. A number of investigators have used it to distinguish those patients who are at a high risk of serious disease (ulcer or cancer) from those who are not. Such findings have important implications for patient management, both in clinical and economic terms.

Dyspepsia↗