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Biomedical subjects

T J Liu

Publications and source records attributed to T J Liu.

At least 127 records · Page 7Linked to original sources
PubMed1988

Differential expression of individual members of the histone multigene family due to sequences in the 5' and 3' regions of the genes.

Histone proteins are encoded by a multigene family. The H3.2(614) and H2a(614) genes are present as single copies which are expressed at high levels, accounting for 30 to 40% of the H3 and H2a mRNAs, respectively, in different types of mouse cells. The other genes which have been isolated each contribute only a very small amount to the total type-specific mRNA pool. We demonstrate here that the differences in the level of expression of these genes are partly due to differences in their transcription rates. To investigate the sequences responsible for these differences in expression among the members of each family, we carried out DNA-mediated gene transfer experiments with both intact and chimeric histone genes. The 5' region of a highly expressed gene [H3.2(614) or H2a(614)] was attached to the 3' region of a histone gene which was expressed at low levels (H3-221 or H2a-291) and vice versa. The results show that sequences in both the 5' and 3' regions of the H3.2(614) and H2a(614) genes contribute to their high level of mRNA production by two independent mechanisms. The effect of the 3' sequences on mRNA accumulation has been narrowed to a 65-base-pair region including the 3'-terminal palindrome and downstream signal implicated in mRNA processing.

Animals↗
PubMed1988

Pseudoaneurysm of the cystic artery with upper gastrointestinal hemorrhage. Case report.

A rare case of pseudoaneurysm of the cystic artery caused by acute cholecystitis is reported. During the severe inflammatory process the common bile duct, duodenum and transverse colon were eroded, in addition to the cystic artery. Findings at computed tomography prompted celiac angiography, which confirmed the preoperative diagnosis. Surgery was successful.

Aneurysm↗
PubMed1987

Mouse histone H2A and H2B genes: four functional genes and a pseudogene undergoing gene conversion with a closely linked functional gene.

The sequence of five mouse histone genes, two H2a and three H2b genes on chromosome 13 has been determined. The three H2b genes all code for different proteins, each differing in two amino acids from the others. The H2b specific elements present 5' to H2b genes from other species are present in all three mouse H2b genes. All three H2b genes are expressed in the same relative amounts in three different mouse cell lines and fetal mice. The H2b gene with the H2b specific sequence closest to the TATAA sequence is expressed in the highest amount. One of the H2a genes lacks the first 9 amino acids, the promoter region, the last 3 amino acids and contains an altered 3' end sequence. Despite these multiple defects, there is only one nucleotide change between the two H2a genes from codon 9 to 126. This indicates that a recent gene conversion has occurred between these two genes. The similarity of the nucleotide sequences in the coding regions of mouse histone genes is probably due to gene conversion events targeted precisely at the coding region.

Animals↗
PubMed1986

An unusual ganglion communicating with hip joint resembling a femoral hernia. Case report.

A rare case of a ganglion communicating with the hip joint was encountered in a 72-year-old man who presented a reducible soft mass over the left femoral triangle. This mass, clinically considered to be a femoral hernia, proved to be a ganglion after excision. We report the clinical course of this case with the suggestion that caution should be given to the patient's subtle symptoms while herniography may be helpful for differential diagnosis of this rare condition.

Aged↗
PubMed1985

Alcohol and social behavior II: the helpful drunkard.

Can alcohol make people more helpful, and if so, how? We hypothesized that alcohol would increase helping when, if the person were sober, the helping response would be under high inhibitory conflict--that is, when it would be affected by strong instigating and inhibiting pressures. Alcohol's damage to inhibitory processing should then allow instigating pressures to have more influence on the response, increasing helping. We expected that under low inhibitory conflict, when either or both of these response pressures would be weak under sobriety, alcohol would have little effect on helping. In two experiments we examined this reasoning. In Study 1, a mild dose of alcohol increased helping among high-conflict subjects pressured to help with a task they did not like, but did not increase helping among low-conflict subjects who either liked the task or were weakly pressured to help. In Study 2, a somewhat stronger dose of alcohol increased helping among all high-conflict subjects pressured to help with an undesirable task, yet again had no effect among low-conflict subjects weakly pressured to help. These studies provided an experimental demonstration of the role of inhibitory response conflict in mediating alcohol's social effects, and show that this process generalizes to prosocial behavior. Additional evidence from both experiments helped to rule out alternative explanations concerning drinking expectancies, alcohol's ability to enhance mood, and its ability to make the task more bearable.

Conflict, Psychological↗