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Biomedical subjects

T J Lam

Publications and source records attributed to T J Lam.

At least 55 records · Page 3Linked to original sources
PubMed1998

Incidence of clinical mastitis in dairy herds grouped in three categories by bulk milk somatic cell counts.

Incidence of clinical mastitis was studied in 274 herds grouped in three categories by bulk milk somatic cell count (SCC). Mean incidence rate of clinical mastitis was 0.278, 0.257, and 0.252 cases per 365 cow-days at risk in herds with low (< or = 150,000), medium (150,000 to 250,000), and high (250,000 to 400,000 cells/ml) bulk milk SCC, respectively. The incidence rate of clinical mastitis was not different among the three categories. Variance in the incidence of clinical mastitis among herds increased as bulk milk SCC decreased. Clinical mastitis caused by Gram-negative pathogens, such as Escherichia coli, Klebsiella spp., or Pseudomonas spp., occurred more often in herds with a low bulk milk SCC. Clinical mastitis caused by Staphylococcus aureus, Streptococcus dysgalactiae, and Streptococcus agalactiae occurred more often in herds with a high bulk milk SCC. Systemic signs of illness caused by clinical mastitis occurred more often in herds with a low bulk milk SCC. Both overall culling rate and culling rate for clinical mastitis were not different among groups catergorized by bulk milk SCC. In herds with a high bulk milk SCC, however, more cows that produced milk with a high SCC were culled. In herds with a low bulk milk SCC, more cows were culled for teat lesions, milkability, udder shape, fertility, and character than were cows in herds with a high bulk milk SCC. In herds with a low bulk milk SCC, cows were also culled more for export and production reasons.

Animals↗
PubMed1998

Management practices associated with low, medium, and high somatic cell counts in bulk milk.

Management practices associated with bulk milk somatic cell counts (SCC) were studied for 201 dairy herds grouped into three categories according to bulk milk SCC. The cumulative production of fat-corrected milk over 305 d of lactation and category for bulk milk SCC were highly correlated; herds within the low category had the highest milk production. Differences in bulk milk SCC among the categories were well explained by the management practices studied. This correlation was not only true for the difference between the high (250,000 to 400,000) and low (< or = 150,000) categories for bulk milk SCC but also for the difference between the medium (150,000 to 250,000) and low categories and the high and medium categories. Management practices that are known to be important for herds in the high category for bulk milk SCC, such as dry cow treatment, milking technique, postmilking teat disinfection, and antibiotic treatment of clinical mastitis, were also found to be important in the explanation of the difference between herds in the medium and low categories for bulk milk SCC. More attention was paid to hygiene for herds in the low category than for herds in the medium or high category. Supplementation of the diet with minerals occurred more frequently for cows in the low category for bulk milk SCC than for cows in the medium and high categories.

Animals↗
PubMed1997

[Machine milking and udder health: a literature review].

This article reviews the influence of machine milking on udder health. The main risks are transmission and penetration of pathogens during milking. Pathogens can be transmitted via the hands, cloths, and liners. Irregular fluctuations in the vacuum can cause penetration of mastitis pathogens into or through the teat canal. Such fluctuations are caused by air blasts in the milking machine. Machine milking can also cause teat lesions, although only severe lesions give rise to new infections. Preliminary research results on the influence of the frequency and degree of udder evacuation show that the clinical symptoms of mastitis generally decreases as the frequency and completeness of milking increase. It is concluded that machine milking can influence udder health but that the influence strongly depends on the exposure to pathogens and the quality of the milker.

Animal Husbandry↗
PubMed1997

[A closer look at the milking machine].

Machine milking has changed enormously in the past decades. The simple milking machine developed at the end of the nineteenth century has become a complex appliance which removes milk from the udder in a fast and efficient way. There is no doubt about the importance of a well functioning milking machine as regards milk quality and udder health. This article reviews the literature on the basic mechanics of machine milking, with special emphasis on the action of the cluster during milking. The movement of the teat cup liner is particularly important in influencing the efficiency of milk extraction and udder health. Therefore special attention is paid to the operation of the liner, how it works, and how the opening and closing of the liner affects the milking process. The functioning of the pulsator, the vacuum fluctuations occurring during milking, and the effect of these fluctuations on the opening and closing of the liner are discussed. The maintenance of the milking machine is also described.

Animal Husbandry↗
PubMed1997

Biosynthesis in vivo and excretion of cortisol by fish larvae.

There is a posthatching rise in levels of endogenous cortisol during the ontogeny of those teleosts studied to date. This is thought to be the result of de novo synthesis of cortisol by the larvae, although there is no direct evidence for this. The present study aimed to demonstrate this process in Asian seabass (Lates calcarifer). Larvae (4 days posthatching) were maintained for up to 12 hours in seawater containing [3H]17 alpha-hydroxyprogesterone. High performance liquid chromatography analysis of extracts of the medium, before and after treatment with glucuronidase, indicates conversion of the precursor to several metabolites. One of these was identified as cortisol on the basis of its isopolarity with authentic standard in thin-layer chromatography, and confirmed by recrystallisation to constant specific activity. Immunohistochemistry on siblings shows that the interrenals are immunoreactive for adrenodoxin (adrenal ferredoxin) and cytochrome P-450(21) (steroid 21-monooxygenase [steroid, hydrogen-donor:oxygen oxidoreductase, 21-hydroxylating]; EC 1.14.99.10), and the pituitary for adrenocorticotrophic hormone. These findings suggest that the pituitary-interrenal axis is functional even at this early stage, and are consistent with the hypothesis that the posthatching rise in endogenous cortisol levels is the result of de novo steroidogenesis.

Animals↗
PubMed1997

Hypothalamic regulation of the pituitary-thyroid axis in the tilapia Oreochromis mossambicus.

Electrolytic lesioning of the preoptic area resulted in an increase in plasma thyroxine (T4) and reverse triiodothyronine (rT3) 10 days later; plasma triiodothyronine (T3) levels were not affected, so that there was also a significant decrease in the T3:T4, but not rT3:T4, ratios. No significant changes in T4, T3, or rT3 levels were observed in fish with lesions in either the anterior or posterior portions of the lateral tuberal nucleus. The pituitary contents of growth hormone and the two prolactins were not affected by any lesion. This indicates that the preoptic area may play a role in the inhibitory regulation of the pituitary-thyroid axis in Oreochromis mossambicus, presumably by way of effects on thyrotropin secretion.

Animals↗
PubMed1997

Variation in infection levels with gastrointestinal nematodes in first-year grazing calves in The Netherlands.

ELISAs with crude adult Ostertagia and crude adult Cooperia antigens were used in a survey to monitor the levels of gastrointestinal nematode infections of 21 dairy calf herds. This survey was performed at the end of the grazing season of 1994 in The Netherlands. The mean extinction value of antibody levels of each herd was compared with those of four groups of reference sera of animals that had experienced moderate infection levels (one group), low infection levels (two groups) or no infection (permanently housed group). This was based on faecal examination, pasture larval counts, serum pepsinogen values and worm counts in tracer and main trial calves. The results of the ELISAs indicated a wide variation in infection levels. Most herds (90%) had infection levels equal to, or even lower than, those of the low infection-reference groups. Herds that had been moved every 2 or 3 weeks had lower infections than set stocked herds. The length of the grazing season showed a positive correlation with Ostertagia, but not Cooperia, antibody titres. There was no evident correlation between infection levels and anthelmintic treatment. The implications of these observations in relation to management and nematode control are discussed.

Animal Feed↗
PubMed1997

The effect of discontinuation of postmilking teat disinfection in low somatic cell count herds. I. Incidence of clinical mastitis.

Results are described of a split-udder trial on the effect of discontinuation of postmilking teat disinfection on the incidence of clinical mastitis in seven dairy herds with a low bulk milk somatic cell count and a high incidence of clinical mastitis. Overall incidence of clinical mastitis was non-significantly lower (18%), whereas the incidence of the most prevalent pathogen associated with clinical mastitis, Escherichia coli, was significantly lower in quarters for which postmilking teat disinfection was discontinued. We concluded that discontinuation of postmilking teat disinfection may decrease the incidence of clinical Escherichia coli mastitis in herds for which standard mastitis prevention measures are executed adequately, bulk milk somatic cell count is low, and incidence of clinical mastitis is high. However, because an increase in intramammary infections with contagious pathogens may occur, care is recommended when advising discontinuation of postmilking teat disinfection.

Animals↗
PubMed1997

The effect of discontinuation of postmilking teat disinfection in low somatic cell count herds. II. Dynamics of intramammary infections.

Results of a 20 month split-udder trial on the effect of discontinuation of postmilking teat disinfection on intramammary infections (IMI) with major and minor pathogens in seven dairy herds with a low somatic cell count are described. The incidence of Escherichia coli IMI was found to be significantly lower, whereas the incidence of IMI with Staphylococcus aureus and minor pathogens was significantly higher in quarters for which postmilking teat disinfection was discontinued than in disinfected quarters. It was concluded that discontinuation of postmilking teat disinfection decreased the incidence of E. coli IMI, accompanied by a, from a practical point of view, acceptable rise in somatic cell count. However, the possible increase in the incidence of S. aureus IMI calls for careful monitoring of the dynamics of IMI with contagious pathogens, when postmilking teat disinfection is discontinued in an attempt to reduce E. coli mastitis.

Animals↗
PubMed1997

An attempt to control lungworm disease and parasitic gastroenteritis on commercial dairy farms by the use of an adaptation of Michel's dose-and-move system.

On seven dairy farms an attempt was made to control lungworm disease in calves by turnout on a pasture grazed earlier by cows, followed by a move to aftermath and ivermectin treatment 2 months later. Transmission of lungworm was observed on all farms. Lungworm disease occurred on four farms at treatment. Coughing re-occurred on three of these farms in some animals 2 months later. Owing to poor performance between turnout and treatment, weight gain was below the norm on the farm with the highest infections and most severe respiratory signs. On the other farms respiratory signs did not result in poor weight gain. Gastrointestinal nematode infections remained low on all farms. The conclusion is that this dose and move scheme cannot be recommended for the control of lungworm.

Animals↗
PubMed1997

Effect of freezing on somatic cell count of quarter milk samples as determined by a Fossomatic electronic cell counter.

The objective of this study was to determine the effect of freezing and the duration of freezing on somatic cell count (SCC) in milk samples from quarters with no signs of clinical mastitis. Effect was determined by a fluoro-opto-electronic cell counter. Samples were frozen in a commercial freezer at approximately -20 degrees C. The milk samples were thawed at 1, 2, 3, 4, 7, 14, or 28 d. Another batch of samples was frozen 1 d, thawed, frozen again for 1 d, and then thawed. The SCC was determined on the fresh samples and after any one of the freezing periods. On average, the SCC were lower after all freezing periods than they were before freezing. However, the difference in SCC before and after freezing was small and relatively constant on the natural logarithm scale. The decrease in SCC was larger with longer freezing periods. When SCC thresholds of 200,000, 250,000, and 500,000 cells/ml were used to predict infection, freezing appeared to have little impact on the sensitivity and specificity of diagnostic parameters. Therefore, SCC, as determined by a fluoro-opto-electronic cell counter, determined for frozen quarter milk samples can be used in mastitis control and research programs.

Animals↗
PubMed1997

Estimation of interdependence among quarters of the bovine udder with subclinical mastitis and implications for analysis.

Interdependency among udder quarters with subclinical mastitis was evaluated on 150 farms using a total of 35,828 udder quarters. The occurrence of high somatic cell count (SCC) (> 250,000 cells/ml) in 0, 3, and 4 quarters occurred at a higher rate than would be expected based on independence of the quarters. For all bacterial species, intramammary infection in 0, 2, 3, or 4 quarters of the same cow occurred at a higher rate than would be expected based on independence of the quarters. Intramammary infection and high SCC were found less often in front quarters than in rear quarters. High SCC and intramammary infection occurred more often in right front quarters than in left front quarters. High SCC in diagonal quarters occurred at a lower rate than expected. Corynebacterium bovis, Streptococcus agalactiae, and Staphylococcus aureus had the highest intraclass correlation within herd. Streptococcus uberis had a very low intraclass correlation within herd. The intraclass correlation within cow for the natural logarithm of SCC was 0.47. Corynebacterium bovis and Strep. agalactiae had the highest intraclass correlation within cow, and Streptococcus dysgalactiae had the lowest. Analytical methods were proposed to manage the problem of interdependence and its effect on the design or evaluation of field studies on subclinical mastitis.

Animals↗
PubMed1997

Estimation of variance components for somatic cell counts to determine thresholds for uninfected quarters.

The objective of this study was to determine the factors affecting somatic cell count (SCC), to estimate variance components of these factors, and to calculate and evaluate the thresholds for intramammary infection based on SCC. The infection status from 22,467 quarter milk samples from 544 cows in seven herds was determined. Infections status was the most important factor affecting SCC. The increase in SCC was more pronounced for major pathogens than for minor pathogens. Even after adjustment for infection status, the interaction between stage of lactation and parity was significant. For culture-negative samples within a lactation, the shape of the SCC curve was inversely related to the shape of the milk production curve. The shape of the SCC curve was flat for first lactation cows compared with the shape of the SCC curve for cows in subsequent lactations. The effect of clinical mastitis on SCC was significant. The use of SCC thresholds for specific parities and stages of lactation to detect intramammary infection improved quality parameters only slightly over a fixed threshold of 200,000 cells/ml.

Animals↗
PubMed1997

Effect of natural infection with minor pathogens on susceptibility to natural infection with major pathogens in the bovine mammary gland.

OBJECTIVE: To evaluate the effect of natural udder infection with minor pathogens on subsequent natural infection with major pathogens. SAMPLE POPULATION: 7 dairy herds with low bulk milk somatic cell count. PROCEDURE: During a 20-month prospective study, milk samples were collected from diary cows at regular intervals and from quarters with clinical signs of mastitis. Incidence of intramammary infection was calculated in uninfected quarters and in quarters infected with minor pathogens. A within-cow, matched case-control analysis was used to evaluate the effect of minor pathogens on subsequent infection with major pathogens. RESULTS: Quarters infected with minor pathogens had higher somatic cell count than did uninfected quarters. In quarters infected with Corynebacterium bovis, the rate of infection with major pathogens was lower, whereas in quarters infected with coagulase-negative Micrococcaceae, the rate of infection with major pathogens was higher than that in uninfected quarters. From the within-cow comparison, it appeared that, in quarters infected with minor pathogens, infection with major pathogens was significantly lower than that in comparable control quarters not infected with minor pathogens. CONCLUSIONS: Minor pathogens have a protective effect against infection with major pathogens. The protective effect of C bovis against subsequent infection with major pathogens appears to be greater than the effect of coagulase-negative Micrococcaceae.

Animals↗
PubMed1996

Transcription regulatory signals in the 5' and 3' regions of Oreochromis aureus ER gene.

The Oreochromis aureus estrogen receptor (OaER) 5' region is 62.2% AT-rich and does not display well-positioned consensus TATA and CAAT boxes. A functional imperfect 13 bp ERE, TGTTAtggTGACC, deviating at 2 bases is located in the leader exon. Transient transfection assays indicate that this ERE confers a 5-fold increase in SEAP reporter gene activity at 20 h post E2-induction. The continued elevation of transcription after the initial peak could be responsible for the "memory' effect of E2-induced vitellogenesis. The 4.4 kb OaER 3' region is AT-rich and has a high representation of 1/2 EREs and GREs. There are 10 copies of the destabilizing pentamer ATTTA which, in transient transfection experiments strongly suppressed SEAP activity. The 3' EREs are functional, and on their own, induce a bimodal increase in SEAP activity of 9-fold at 6 h and 11-fold at 18 h post-E2 induction. A recombinant construct of SEAP gene flanked by the 5' upstream and 3' flanking regions of the OaER gene allowed these regulatory signals to "cross-talk' to achieve a 5- and 25-fold increase for the first and second peaks, respectively. This bimodal response to E2-induction is attributed to both transcriptional and translational controls over the SEAP gene. This work represents a novel illustration of a synergistic interaction between the 5' and 3' regulatory elements of a steroid receptor in autoregulation.

Animals↗
PubMed1996

The value of bulk milk ELISA Ostertagia antibody titres as indicators of milk production response to anthelmintic treatment in the dry period.

An experiment was done to study whether estimation of the herd infection level, by assessing Ostertagia antibodies in bulk milk samples can serve to predict the effect of anthelmintic treatment on milk production. Bulk milk samples were collected from 134 farms at monthly intervals on three occasions prior to the start of the study. The ELISA titres to Ostertagia found in October at the end of the grazing season served as a basis to select 16 and 18 farms as having high and low levels of parasitism respectively. Heifers and cows within each herd were ranked by expected calving date, paired and randomly allocated to be treated with ivermectin or a placebo. Records of milk production and composition were collected for all the trial animals. The response to treatment expressed as the 305 day corrected milk yield of anthelmintic treated animals minus that of placebo treated animals was not statistically significant and amounted to 78 kg for multiparous cows and 124 kg for heifers. For cows as well as heifers the response to treatment was larger in the high antibody level herds than in low antibody level herds, but these differences also lacked statistical significance.

Animals↗
PubMed1996

Comparison between fenbendazole and moxidectin applied in a dose and move system for the control of Dictyocaulus viviparus infections in calves.

A grazing study was performed with the main objective to compare the effect of moxidectin (MDT) and fenbendazole (FBZ) in a 'dose and move' system on nematode infections in calves with special emphasis on Dictyocaulus viviparus. Three groups of six calves were grazed from May to October 1994. All groups grazed together until 9 weeks after turnout when they were moved to separate mowed pastures. One group (MM) was then treated with 0.5 mg kg-1 MDT pour-on, the second group (FM) was treated with 7.5 mg kg-1 FBZ drench and the third group served as untreated pasture control group (PC). Two calves from MM and two from FM were experimentally infected with 20 lungworm larvae at turnout in order to initiate low infections in the herd. Pairs of tracer calves grazing during the first 7, 8 or 9 weeks after turnout acquired mean burdens of 218, 255 and 1156 lungworms, respectively. MDT and FBZ treatment removed adult lungworms from MM and FM. In PC faecal larval counts increased until the end of July, when most animals were suffering from lungworm disease. No lungworm disease occurred in both dose and move groups. In FM larvae reappeared in the faeces of some of the calves from 1 month after treatment and low patent infections remained to be present in FM in some calves until the end of the experiment. No reappearance of larvae after treatment was observed in MM. The mean Optical Density (OD) values of the three groups on pasture closely followed the infection patterns. After housing in October all calves, and also a group of five permanently housed non-infected control calves (HC), were infected experimentally with 5000 D. viviparus larvae to evaluate development of immunity. The worm counts at necropsy showed that groups on pasture had developed immunity. However, the degree of immunity was lower in MM than in FM and PC.

Animal Husbandry↗