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T J Kelly

Publications and source records attributed to T J Kelly.

At least 145 records · Page 8Linked to original sources
PubMed1979

Evolutionary relationships of the primate papovaviruses: base sequence homology among the genomes of simian virus 40, stump-tailed macaque virus, and SA12 virus.

Physical maps of the genomes of the two newly discovered primate papovaviruses, SA12 and stump-tailed macaque virus (STMV), were generated by restriction endonuclease analysis. The base sequence homologies among the genomes of SA12, stump-tailed macaque virus, and simian virus 40 (SV40) were studied by heteroduplex analysis. Heteroduplexes between SA12 and SV40 DNAs and stump-tailed macaque virus and SV40 DNAs were constructed and mounted for electron microscopy in various amounts of formamide to achieve a range of effective temperatures. At each effective temperature, the regions of duplex DNA in the heteroduplexes were measured and localized on the SV40 physical and functional maps. By analyzing the data from this study and rom our previous study (N. Newell, C. J. Lai, G. Khoury, and T. J. Kelly Jr., J. Virol. 25:193-201, 1978) on the base sequence homology between the genomes of BK virus and SV40, some general conclusions have been drawn concerning the evolutionary relationships among the genomes of the primate papovaviruses. The extent of homology among the viral genomes does not reflect the phylogenetic relationships of their hosts. At comparable effective temperatures Tm - 33 degrees C), the heteroduplexes between the DNAs of BK virus and SV40 contained the largest amount of duplex (about 90%). The heteroduplexes made between SA12 and SV40 DNAs were slightly less homologous, containing about 80% duplex. The heteroduplexes made between SV40 and stump-tailed macaque virus DNAs were only 20% duplex under the same conditions. When the various heteroduplexes were mounted for microscopy at effective temperatures greater than Tm - 33 degrees C, the fraction of the duplex DNA decreased in each case, indicating the existence of considerable base mismatching in the homologous regions. When specific coding or noncoding regions of the viral genomes were compared, the data indicated that the extent of sequence divergence differed markedly from one region to another. In all the heteroduplexes studied, there were two regions, located near the junctions between early and late regions on the SV40 map, which were essentially nonhomologous. All of the heteroduplexes studied showed significantly greater homology in the late region than in early region. Within the late region, the sequences coding for the major capsid polypeptide, VP1, were the most highly conserved.

Base Sequence↗
PubMed1978

Electron microscope study of the base sequence homology between simian virus 40 and human papovavirus BK.

The base sequence homology between the genomes of simian virus 40 (SV40) and human papovavirus BK (BKV) was studied by the heteroduplex method of Ferguson and Davis (J. Mol. Biol. 94:135-149, 1975). When mounted for microscopy in 30% formamide (Tm-35 degrees C), BKV/SV40 heteroduplexes were an average of 92% double-stranded and contained only two small nonhomologous regions that mapped near the junctions between the early and late regions of the SV40 Genome. At higher formamide concentrations, the fraction of duplex DNA in the BKV/SV40 heteroduplexes decreased, indicating significant base mismatching in the homologous regions. The strongest regions of homology were located in the late region.

BK Virus↗
PubMed1977

Immunological relatedness of papovaviruses of the simian virus 40-polyoma subgroup.

Viral antigens in permissive cells infected with JC virus, K virus, and SA12 virus were reactive in immunofluorescence tests to antisera against sodium dodecyl sulfate-disrupted simian virus 40 capsids and polyoma VP1. The major capsid polypeptides of all papovaviruses of the simian virus 40-polyoma subgroup are immunologically related.

Antigens, Viral↗
PubMed1977

Common structural antigen of papovaviruses of the simian virus 40-polyoma subgroup.

An antigenic determinant common to the major capsid polypeptide (VP1) of simian virus 40 (SV40) and polyoma virus is described. Antisera prepared against intact viral particles reacted only with cells infected with the homologous virus by immunofluorescence tests (IF). However, antisera prepared against disrupted SV40 particles reacted in IF with both polyoma- and SV40-infected permissive cells. The cross-reaction with polyoma was localized to VP1 by the following evidence. (i) The IF cross-reaction was inhibited by preincubation of the antiserum with purified SV40 VP1; (ii) purified radiolabeled polyoma VP1 was precipitated by the cross-reactive serum, and this reaction was inhibited by unlabeled SV40 VP1; (iii) other antisera prepared against purified SV40 VP1 or polyoma VP1 reacted in IF with both SV40- and polyma-infected permissive cells. These cross-reacting antisera also reacted in IF with permissive cells infected with BK virus, rabbit kidney vacuolating virus, and the stumptailed macaque virus, suggesting that all members of the polyoma-SV40 subgroup share a common antigenic determinant located in their major capsid polypeptides.

Antigens, Viral↗
PubMed1976

Integrated simian virus 40 sequences in transformed cell DNA: analysis using restriction endonucleases.

The DNAs from five independent simian virus 40 (SV40) transformants of the BALB/c3T3 mouse cell line were digested with either the HpaII or the BamHI restriction endonuclease and the resulting fragments were fractionated by gel electrophoresis. The DNA fragments were denatured in situ in the gel and transferred to a membrane filter. Fragments containing viral DNA were detected by hybridization with high specific activity 32P-labeled SV40 complementary RNA (cRNA) synthesized in vitro. Each of the lines yielded a small number of fragments containing SV40 DNA and the fragments from each line were different. This observation shows that the structure of the integrated SV40 DNA and/or its location in the host DNA are different in each line.

Cell Line↗
PubMed1976

Identification of the stumptailed macaque virus as a new papovavirus.

A new viral agent, stumptailed macaque virus (STMV), isolated from uninoculated stumptailed macaque kidney cultures was identified. The virions had the size and morphology of papovaviruses of the simian virus 40 (SV40)-polyoma subgroup, but many of them appeared to have an additional outer envelope. The deoxyribonucleic acid of STMV was a superhelical circular molecule, with a mean length 91% of that of SV40. The antigenic relationship of this virus with other members of the group was examined by immune electron microscopy of isolated virions and by immunofluorescent staining of virus-infected cells. STMV was immunologically distinct from SV40, BK virus (BKV), polyoma virus, and JC virus. Its tumor antigen may be related to those of SV40 and BKV.

Animals↗
PubMed1975

Structure of the DNA of the adenovirus 7-simian virus 40 hybrid, e46, by electron microscopy.

The DNA molecule of the adenovirus 7-simian virus (SV) 40 hybrid, E46(+), contains a single substitution of SV40 DNA located about 0.05 adenovirus 7 lengths from one end (arbitrarily designated the left end). The left to right direction in the SV40 DNA segment of the hybrid corresponds to the clockwise direction in the SV40 physical map. The left end point of the segment maps at about SV40 map position 0.50 +/- 0.01 and the right end point at about SV40 map position 0.66 +/- 0.01. The region between SV40 map positions 0.71 and 0.11 (clockwise) is deleted. Thus, the region between SV40 map position 0.50 and 0.66 (clockwise) is repeated in tandem.

Journal Article↗