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Biomedical subjects

T Iwasaka

Publications and source records attributed to T Iwasaka.

At least 307 records · Page 17Linked to original sources
PubMed1984

[Evaluation of cervical conization as a definitive treatment for borderline lesions of the uterine cervix].

Therapeutic efficacy of cervical conization was examined on 93 patients with borderline lesions of the uterine cervix who underwent hysterectomy following conization. No lesions or only minimal residual lesions were found at subsequent hysterectomy when the surgical margins of cone specimens were not involved in the malignant or premalignant lesions. From these results, we concluded that cervical conization could be considered as a therapeutic procedure when the following conditions were fulfilled. Cone specimens are sufficient in size. Both endocervical and exocervical margins are negative. No malignancy is found on endocervical curettage. No abnormal cytologic findings are found after conization. Another follow-up study was performed on 69 patients who received therapeutic conization between 1971 and 1981. Among them one developed stage II cervical cancer two years after the last follow up. Two other patients had recurrent CIN in the cervix and another patient was found to have an endometrial cancer. These patients were successfully treated after early detection of the lesions by the cytological examination. From these facts, cytological examination is recommended in the follow-up after therapeutic conization.

Adenocarcinoma↗
PubMed1983

Immunity to herpes simplex virus type 2: recurrent lesions are associated with the induction of suppressor cells and soluble suppressor factors.

Cell-mediated immunity to herpes simplex virus type 2 was investigated in infected inbred strain 13/N guinea pigs with (45%), and without, a history of recurrent herpetic disease (A. D. Donnenberg, E. Chaikof, and L. Aurelian, Infect. Immun. 30:99-109, 1980). Induction of suppressor cells capable of reducing the proliferative response of herpes simplex virus type 2-stimulated immune lymphoid cells was demonstrated in spleen cells from animals with a history of recurrent disease at recrudescence and convalescence but not in spleen cells from quiescent animals or from animals without a history of recurrent herpetic disease (seropositive controls). Suppressor cells were also detected in the peripheral blood but only from three of seven studied animals, and only at recrudescence. In addition to inhibitory cell-cell interactions, the herpes simplex virus type 2-activated regulatory cells of animals with recrudescent herpetic lesions elaborated soluble suppressor factors affecting lymphocyte proliferation. Suppression mediated by suppressor factors was observed only when suppressor factors were added at an early stage of in vitro culture and was reversed by medium exchange throughout the 6 days of culture. Sephadex chromatography revealed the presence of factors capable of differentially modulating the proliferative response of herpes simplex virus-stimulated immune cells and concanavalin A-stimulated normal lymphoid cells.

Animals↗
PubMed1982

[Over diagnosis of the biopsy: from the analysis of the extirpated uterus after radical hysterectomy (author's transl)].

From January, 1973 to December, 1980, 396 cases of cervical cancer were treated with radical hysterectomy under the diagnoses of stage Ib, IIa and IIb. Precise postoperative pathological examinations about the extirpated uterus revealed that 63 of them had only early invasive cancer or less advanced lesions though they had been considered as frank invasive cancer by preoperatively performed biopsies. The major causes of the misinterpretations were, 1) misjudging the glandular involvements as frank invasive lesions, or 2) misjudging the tangentially sectioned lesions of the bulky outgrowth as frank invasions. Furthermore, it is sometimes difficult to identify the invasive lesions in the biopsy specimens whether they are early invasive or frank invasive. Colposcopic and cytological examinations were simultaneously performed to each cases. Either cytology or colposcopy did not suggest frank invasions in 44.2% of cases. Both of them did not suggest frank invasions in 37.2% of cases. At least, cone biopsy should have been performed to identify the degree of invasions of these 37.2% cases. The authors emphasize that the directed biopsies are not always accurate to diagnose the early invasions, and one should use colposcopy, cytology and directed biopsy to make an accurate diagnosis.

Adenocarcinoma↗
PubMed1980

Tubular structures in the cervix of mice experimentally infected with herpes simplex virus type 2.

Experimental infection of the C3H/N mouse genital tract was demonstrated after intravaginal inoculation with herpes simplex virus type 2 (HSV-2). About 75% of the infected animals died by Day 7, and 75% of the surviving animals had severe vaginitis or neurological signs on Day 7. Titers of the virus recovered from vaginal secretions of infected animals reached a maximum on Day 2 and gradually decreased until Day 7. On the other hand, under the electron microscope, virus particles and tubular structures could be found in the nuclei of infected cells of the cervix in the 1st, 2nd and 4th days after infection. All cases in which virus particles could be found in the nuclei of infected cells were also positive for tubular structures and vice versa. These observations indicate that in situ diagnosis of HSV-2 infection can be made in the mouse model. The same method would be applicable for the diagnosis of human HSV-2 infection.

Animals↗
PubMed1979

Effects of antimetabolites on the production of tubular structures in Vero cells infected with herpes simplex virus type 2.

Treatment of Vero cells infected with herpes simplex virus type 2 with the proper concentrations of hydroxyurea (HU) reduced the production of infectious virus and markedly increased the accumulation of tubular structures in the nuclei when the drug was added within 6 hours after infection. Similar accumulation of tubular structures in the infected nuclei was also observed when infected cells were treated with phosphonoacetic acid at proper concentrations. The release of HU-treated, herpes simplex virus type 2-infected cells from the drug-induced blocking of synthesis of infectious virus resulted in the marked decrease of tubular structures coincidentally with the beginning of production of infectious virus. The data suggest the possibility that the disappearance of tubular structures may be related to the active production of infectious virus.

Animals↗
PubMed1979

Mycoplasma infection and infertility.

Attempts were made to isolate mycoplasmas from the uterine cervix of infertile women and normal pregnant and nonpregnant women to investigate the relationship of genital mycoplasma infection to infertility. Ureaplasma urealyticum was demonstrated in 63% of patients with infertility, 68% of normal pregnant women, and 62% of normal nonpregnant women. The incidence of Mycoplasma hominis infection was found to be noticeably lower, with corresponding isolation rates of 10%, 11%, and 6%, respectively. The differences in rate of isolation for U. urealyticum and for M. hominis among the three groups did not reach statistical significance. During a follow-up period of more than 12 months without any treatment for mycoplasma infection in the infertile cases, 11 women (27.5%) became pregnant. These included 7 (28%) of 25 women with positive U. urealyticum cultures. The results demonstrate a fairly high incidence of female genital infection with U. urealyticum which, as the data would indicate, cannot be directly associated with infertility.

Adult↗
PubMed1979

Production of tubular structures in Vero cells infected with herpes simplex virus type 2; effects of ultraviolet light irradiation and antimetabolites.

In order to investigate the nature of tubular structures specifically found in herpes simplex virus type 2 (HSV-2)-infected cells, the multiplication of HSV-2 was studied in Vero cells cultured in the presence of varying concentrations of cytosine arabinoside (Ara-C) and cycloheximide (CH), inhibitors of DNA synthesis and protein synthesis respectively. Ara-C, at a concentration of 60 micrograms/ml, inhibited the multiplication of HSV-2 by more than 99% and also prevented the appearance of tubular structures and virus particles in the nuclei of infected cells. Nevertheless, the synthesis of virus specific surface antigens of HSV-2-infected Vero cells was not reduced, as revealed by the fluorescent antibody technique. On the other hand, 10 micrograms/ml of CH inhibited both the appearance of tubular structures and virus particles and the synthesis of virus specific surface antigens by more than 99%. These observations strongly suggest that the appearance of tubular structures is one of the late events in the process of virus multiplication. To measure the comparative genome size needed to produce membrane antigens, tubular structures and infectious centres, the effect of u.v.-inactivation of HSV-2 on these processes was studied. After u.v.-irradiation, the capacity to induce tubular structures was inactivated at a slower rate than the capacity to form infectious centres, but at a faster rate than the induction of surface antigens. Furthermore, more tubular structures could be induced by u.v.-inactivated virus than by the non-irradiated virus which was diluted to the same infectivity as the u.v.-irradiated virus. These results indicate that expression of the entire genome is not required for the production of tubular structures.

Animals↗
PubMedDate not supplied

Urinary excretion of biopyrrins, oxidative metabolites of bilirubin, increases after spasm provocation tests in patients with coronary spastic angina.

BACKGROUND: Bilirubin apparently functions as an antioxidant in vivo by reacting with reactive oxygen species, and, as a result, becomes oxidized. The urinary excretion of oxidative metabolites of bilirubin, biopyrrins, could be a biological marker for in vivo production of reactive oxygen species. The purpose of this study was to examine the extent of oxidative stress in patients with possible ischemic heart diseases (n=44) by measuring urinary biopyrrins by enzyme-linked immunosorbent assay before and after the spasm provocation test (SPT). METHODS: Spot urine samples were collected five times; 1 day before, in the morning just before, immediately after, 6 h after, and 1 day after the SPT. Nineteen patients were positive to SPT judged from the specific changes in electrocardiogram for myocardial ischemia following intracoronary injections of ergonovine. RESULTS: The baseline data such as age, sex, number of risk factors and concentrations of serum bilirubin, and the measured hemodynamic parameters of heart rate, blood pressure, left ventricular end-diastolic pressure and left ventricular ejection fraction were not different between the positive and negative groups. The baseline concentrations of biopyrrins during the control period were not significantly different between the two groups. However, they increased significantly after the SPT, thereby the magnitude of increases immediately after and 6 h after the SPT were significantly (P<0.001 and P<0.01, respectively) greater in the positive group than in the negative. CONCLUSION: The present findings strongly suggest that coronary arterial occlusion augments production of biopyrrins, which indicates exposure to oxidative stress in patients with ischemic heart diseases.

Adult↗
PubMedDate not supplied

Endometrial extension of adenosquamous carcinoma of the uterine cervix.

Endometrial in situ extension of cervical cancer is extremely uncommon. Previous reports only present the cases of squamous cell carcinoma or related category. This report presented adenosquamous carcinoma of the uterine cervix that showed a paradoxical extension of each component in a 72-year-old patient. Main tumor in the cervix was revealed to be adenosquamous carcinoma. The glandular component extended to the vagina, while the squamous component grew into the entire uterine cavity and replaced the glandular epithelium. We presented the first case of adenosquamous carcinoma of the uterine cervix with vaginal and endometrial extension. Furthermore, the endometrium was replaced with squamous component, while the vagina was invaded by glandular component. The observed paradoxical extension of the present case was extremely rare.

Aged↗