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Biomedical subjects

T Inagaki

Publications and source records attributed to T Inagaki.

At least 289 records · Page 16Linked to original sources
PubMed1975

Albumin synthesis and effect of betamethasone on albumin synthesis in perfused liver of normal and CCL4-intoxicated rats.

Synthesis of plasma albumin was studied in perfused liver of normal and CCl4-intoxicated rats, using 14C-bicarbonate method. 1. In normal liver, albumin synthesis averaged 5.6 mg/hr/100 g body weight. 2. In CCl4-intoxicated liver, albumin synthesis decreased to 4.6 mg at acute stage, but restored to 5.1 mg at non-septal fibrotic stage. At septal fibrotic, cirrhotic and cirrhotic stage with ascites, the synthesis rate averaged 4.5 mg, 4.1 mg and 3.2 mg, respectively. From these results, it is inferred that albumin synthesis rate decreases in accordance with the progress of the liver injury. 3. Effect of betamethasone on plasma albumin synthesis in perfused rat liver was investigated. Albumin synthesis rate in normal liver averaged 5.4 mg, and after addition of betamethasone, the rate averaged 4.3 mg, 3.3 mg and 2.8 mg, respectively. The rate after addition of betamethasone increased to 7.3 mg, 5.5 mg and 4.5 mg, respectively. From these results obtained, it is inferred that betamethasone increases plasma albumin synthesis in both normal and CCl4-intoxicated liver. 4. In normal liver, actinomycin-D did not inhibit basal albumin synthesis, but inhibited the increase of albumin synthesis by betamethasone, when actinomycin-D was added together with betamethasone. From these facts, it is concluded that betamethasone is able to induce albumin synthesis.

Animals↗
PubMed1975

Clinico-pathological studies of the liver in asymptomatic carriers of Australia antigen.

In an attempt to clinico-pathologically examine asymptomatic HBs AG carriers, follow-up studies were made on 57 HBAg-positive blood donors with the S-GPT within normal range and the following conclusions were arrived at: 1) The results of liver function tests made in the present studies revealed the following rates of abnormalities: the S-GPT was abnormal in 14.5% of the subjects, the S-GOT was abnormal in 9%, the serum total bilirubins were abnormal in 12.2%, the serum alkaline phosphatase level was abnormal in 24.5%, the TTT was abnormal in 4.4%, the ZTT was abnormal in 2.2%, the gamma-globulin was abnormal in 21.2%, and the ICG retention was abnormal in 25.6%. It was thus necessary to make a follow-up study of the results of liver function test. 2) Anti-HBs was negative in all subjects, the rate of lymphocytic blastogenesis in the peripheral blood (tested by the addition of PHA) was low in 7 (36.8%) of 19 patients, and the MIF test by the addition of the purified HBs Ag revealed that 17 subjects, excluding one subject with a histologic picture of acute hepatitis, were not susceptible to HBs Ag. It was, therefore, surmised that immunological insufficiency would be involved in the development of asymptomatic carriers. 3) Histologic examinations, made on 20 subjects, revealed A.V.H. in one subject, N.S.R.H. in seven, N.S.R. in ten and fatty liver in two, and further revealed mild, diffuse inflammations in 8 subjects in the first two group (40% in total). Further, pleomorphism was noted in the hepatocytes of 8 (40%) of these 20 subjects, and a study is under way of the significance of the pleomorphism.

Alanine Transaminase↗
PubMedDate not supplied

A low molecular weight binding protein for organic anions (Z protein) from human hepatic cytosol: purification and quantitation.

Human Z protein from liver was purified to homogeneity. The protein has a molecular weight of 11,000 an an isoelectric point of pH 5.8. Circular dichroism spectra of Z protein-bilirubin (unconjugated and diglucuronide) complexes revealed two ellipticity extrema, a negative peak at 460 nm, and a positive peak at 410 nm. Human serum albumin had a higher affinity for bilirubin than did Z protein. Fluorescence studies showed the approximate association constants of this protein and bilirubin, bromosulfophthalein, and indocyanine green were 10(6) M-1, 10(5) M-1, and 10(6) M-1, respectively. Immunofluorescence studies revealed that Z protein was localized in the cytoplasm of hepatocytes, proximal tubular epithelium, and epithelial cells of the small intestine. Radioimmunoassay studies were done to assess the amount of Z protein in controls and in various liver diseases. The highest concentrations of Z protein were found in the liver, kidney, heart muscle, and small intestine, in that order. In cases of acute and chronic hepatitis, hepatic Z protein concentrations were generally decreased, whereas serum Z protein concentrations were increased. In contrast, both serum and hepatic Z protein concentrations were decreased in cases of constitutional hyperbilirubinemia.

Bilirubin↗
PubMedDate not supplied

Intracranial dural cyst.

Intracranial dural cyst is a very rare congenital malformation. The presence of such a cyst was confirmed by means of computed tomography and magnetic resonance findings in a child with an enlargement of the head and developmental retardation and confirmed histologically in surgically obtained specimens of cyst wall.

Arachnoid Cysts↗
PubMedDate not supplied

Atrial natriuretic peptide in aged patients with iron deficiency anemia.

Anemia is a common disease in elderly people. However, since hemoglobin concentration often decreases subclinically with aging because of nutritional impairment, its pathological significance is unclear. To investigate the pathological significance of low hemoglobin concentration, we studied the relation between hemoglobin levels and arrhythmia, as well as circulatory parameters. Arrhythmia was detected by Holter type ambulatory electrocardiography in 42 elderly people (aged 60 or over) living in a nursing home. Plasma concentrations of human atrial natriuretic peptide (hANP) after iron therapy were determined by immunoradiometric assay. Changes in circulatory parameters in elderly people with iron deficiency anemia were examined. Supraventricular and ventricular premature contractions significantly increased in elderly people with low hemoglobin concentrations or hematocrit. hANP increased significantly as the hemoglobin concentration decreased in 22 elderly people. Of these 22 subjects, 11 showed a low serum concentration of iron, and were administered ferrous salts. No side effects, such as nausea, occurred. After iron supplementation, the average hemoglobin level increased from 9.0 to 10.5 g/dl, and the average hANP level was reduced from 58.3+/-23.5 to 41.2+/-27.9 pg/ml, which was statistically significant by Wilcoxon's signed rank sum test. The increase in the hemoglobin level inversely correlated with the hANP level. Heart rate, blood pressure and body weight of subjects decreased significantly after iron supplementation therapy. Although hemoglobin levels were increased by iron supplementation therapy after a long period of anemia, the duration of the period with low hemoglobin levels showed no significant relation to initial hANP concentration. In conclusion, low hemoglobin levels induced secretion of hANP, and treatment of iron deficiency might exert favorable effects on the circulatory system.

Journal Article↗