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Biomedical subjects

T Ikeuchi

Publications and source records attributed to T Ikeuchi.

At least 253 records · Page 14Linked to original sources
PubMed1984

[Combination chemotherapy of mitomycin C and cytosine arabinoside in the intravesical treatment for superficial bladder tumors: report 2. Evaluation of prophylactic intravesical infusion therapy].

Combined chemotherapy of Mitomycin C (20 mg to 30 mg) and Cytosine Arabinoside (200 mg) was given by intravesical infusion to 25 patients with superficial bladder tumor who underwent transurethral treatment for prevention of postoperative recurrence. The prophylactic effect of this therapy was evaluated as follows. 1) The postoperative recurrence rates by the actuarial method were 4.0% in six months, 8.1% in one year, 22.2% in two years, 37.4% in three years and 37.4% in five years. 2) The prophylactic effect on recurrence tended to be marked on primary tumors, solitary tumors, low grade tumors and relatively large tumors. 3) The correlation of doses and the recurrence rate was significantly observed: prophylactic effect tended to increase in proportion to increase of doses of both Mitomycin C and Cytosine Arabinoside.

Adult↗
PubMed1983

Cloning of sporulation genes spo0A and spo0C of Bacillus subtilis onto rho 11 temperate bacteriophage.

A HindIII fragment harboring the intact spo0C gene of Bacillus subtilis was cloned with rho 11 temperate bacteriophage as a vector. Transformation experiments with the DNA from rho 11 dspo0C+ specialized transducing phage showed that the spo0C gene resides on a 5.3-megadalton fragment generated by HindIII digestion. The 5.3-megadalton fragment also contains the intact spo0A gene, but not spoIIIA, spoIIIB, or spoIVB.

Bacillus subtilis↗
PubMed1983

Chemical composition of winged bean (Psophocarpus tetragonolobus) varieties.

The chemical composition of dry seeds of four varieties, pods, stalks and leaves of winged beans (Psophocarpus tetragonolobus) was determined. The seeds had a high range of protein (27.8-36.6%) and fat (14.8-17.9%), which were similar to soybeans. the seeds contained high phosphorus, calcium and magnesium. The leaf was highest in protein content (33.7%) of all the parts studied except for the seeds. The protein and fat content of pods decreased as pods ripened. the calcium content in the leaf was much higher than in the other parts. Protein was extracted sequentially with 2% NaCl, 30% isopropyl alcohol, 4% lactic acid and 0.5% KOH from dry seeds of four varieties of winged beans. The NaCl extract showed the highest range of protein concentration (60.2-77.6%). The NaCl extract was separated into two fractions based on solubility in water. the amino acid composition of the flour from the seeds and of the two fractions from the NaCl extract were determined. Contents of lysine, aspartic acid, glutamic acid and leucine were large, while the sulfur-amino acid content was small. Trypsin and chymotrypsin inhibitory activities of 2% NaCl extract from the seeds were determined, and chymotrypsin inhibitory activity was higher than the trypsin.

Calcium↗
PubMed1983

[Combination chemotherapy of mitomycin C and cytosine arabinoside in superficial bladder tumors. 1. Evaluation of intravesical infusion therapy].

A combined chemotherapy of Mitomycin C (20-30 mg) and Cytosine Arabinoside (200 mg) was given intravesical by to 37 patients with superficial bladder tumor. The antitumor effect of the therapy was evaluated as follows. 1) The results were: remarkably effective in 12 cases (32.5%); effective in, 14 cases (37.8%); non-effective in 11 cases (29.7%), with overall rate of 70.3%. 2) The antitumor effect tended to be evident in multiple tumors, small tumors, and tumors of low malignancy. 3) Side effects were found in 35.1% of the cases including 24.3% of bladder irritating symptom. 4) The follow-up period ranged from 12 to 60 months with an average of 36.6 months. Recurrence rate was 27.0%. There was a tendency that recurrence occurred more frequently in cases of multiple tumors and small tumors than in other cases.

Adult↗
PubMed1982

Effect of paromomycin sulfate on endotoxemia in patients with cirrhosis.

We evaluated, in a randomized double-blind trial, the efficacy of oral paromomycin sulfate administration in the prevention of endotoxemia in 24 cirrhotic patients with endotoxemia. Renal function was evaluated by glomerular-filtration rate and renal plasma flow at the beginning and at the end of the study period. After the administration of paromomycin sulfate, 2 g/day for 4 weeks, endotoxemia disappeared in 10 out of 13 (76.9%) cirrhotic patients with endotoxemia, whereas it became negative in only 3 of the 11 (27.3%) treated with placebo, the difference being significant (P less than 0.05). With regard to correlation of endotoxemia with renal impairment, endogenous creatinine clearance and p-aminohippurate clearance were significantly improved (P less than 0.02) in those patients whose endotoxemia disappeared on paromomycin sulfate administration. We did not find significant improvement, however, neither in liver function or in blood coagulation tests in the same patients. Paromomycin sulfate seems to be effective in the prevention of endotoxemia and the associated renal impairment in cirrhosis in man.

Acute Kidney Injury↗
PubMed1981

Differential inducibility of chromosome aberrations and sister-chromatid exchanges by indirect mutagens in various mammalian cell lines.

A total of 17 cultured mammalian cell lines of various types was investigated for the inducibility of chromosome aberrations (CAs) and sister-chromatid exchanges (SCEs) after exposure to 3 direct mutagens, cyclophosphamide (CP), dimethylnitrosamine (DMN) and benzo[a]pyrene (BP), without extrinsic metabolizing factors. The following cell lines were highly sensitive to CP as estimated by the dose-dependent increase of either CAs or SCEs, or both: 4 rat ascites hepatomas (AH66-B, AH66-C, AH70B, AH109A), 3 rat esophageal tumors (R1, R2, R3), the Yoshida sarcoma (YS), a Chinese hamster embryonic cell line (B-13), and a human embryonic (HE2236) cell line. 1 golden-hamster embryonic (GHE), 1 human embryonic (HE2144) and 1 human esophageal tumor (TH) cell line were moderately sensitive to CP in terms of the yield of SCEs, though they showed rather low sensitivity when measured by the frequency of CAs. 2 Chinese hamster (CHO-K1, CHL) and 1 human lymphoma (P3HR-1) cell lines were much less sensitive or almost insensitive with respect to the yield of both CAs and SCEs. Considering the above results and taking some other experimental conditions into account, 7 cell lines, including another Chinese hamster cell line (Don 6), were similarly investigated with DMN and BP. The results indicated that AH66-B, which was highly sensitive to CP, was also sensitive to DMN and BP, and that the CP-sensitive R1 and R3 were responsive to DMN. While YS, B-13, Don 6 and P3HR-1 did not show any significant response to DMN, a considerable increment of SCEs was noted in B-13 after treatment with BP, GHE was also notable for its high sensitivity to BP, showing an apparently dose-dependent increase of both CAs and SCEs. HE2144 exhibited a slightly increased rate of SCEs after exposure to BP.

Animals↗
PubMed1981

A possible role of R plasmids in bacterial permeability for beta-lactam antibiotics.

Four strains of clinical isolates of Serratia marcescens (13039, 13090, 13093, 14093) harboring R plasmids were highly resistant to ampicillin (ABPC) and cephaloridine (CER). With elimination of R plasmids from these strains by acriflavine treatment, ABPC-resistance levels of these strains were markedly reduced. Reduction of CER-resistance levels was also demonstrated in strains 13039 and 13093, but not in strains 13090 and 14093. The permeability of the former strains for CER was also decreased, but not in the latter strains. At the same time, beta-lactamase activity of these strains also almost completely disappeared when the R plasmids were eliminated. By broth matings with these strains. The recipient strains of S. marcescens 13031 (rif), Escherichia coli K-12 (rif), and E coli 15046 (rif) all acquired a high permeability barrier against CER with inheritance of the R plasmids from strains 13039 and 13093, but not from strains 13090 and 14093. The transconjugant of strain 13031 that inherited R plasmid 13093 was resistant not only to CER but also to cefazolin, cephalothin, and cephalexin. Its permeability to these antibiotics was significantly lower than that of the original strain. This fact suggest the possibility that the R plasmid from strain 13093 may be involved not only in production of beta-lactamases, but also in regulation of bacterial permeability for cephalosporins.

Ampicillin↗
PubMed1979

Genetic evidence for possible interaction between a ribonucleic acid polymerase subunit and the spo0C gene product of Bacillus subtilis.

Spontaneous rifampin-resistant mutants (9V Rifr) were isolated from a mutant strain of Bacillus subtilis, 9V, which has a spo0C mutation. Whereas 90% of the 9V Rifr double mutants maintained the Spo0C phenotype (Spo- Abs +/-), the remaining 10% had the Spo0A phenotype (Spo- Abs-). The latter mutants, termed 9V Rifr Spo- Abs-, were revealed to have other Spo0A characters, such as reduced transformability, higher sensitivity to phage phi 2, and reduced frequency of lysogenization by phage phi 105. The rif mutation of these 9V Rifr Spo- Abs- strains was mapped near the cysA locus. The phenotype of the Rifr transformants of strain 9V by deoxyribonucleic acid derived from these 9V Rifr Spo- Abs- strains was Spo0A, and that of the Rifr transformants of strain 168 was Spo+ Abs+. The ribonucleic acid polymerase of the 9V Rifr Spo- Abs- strains was shown to be resistant to rifampin.

Bacillus subtilis↗
PubMed1979

Relationship between endotoxaemia and protein concentration of ascites in cirrhotic patients.

Endotoxaemia was investigated by the Limulus assay in 42 cirrhotic patients with ascites and in 33 without ascites. The incidence of endotoxaemia in the former group (59.5%) was significantly (P less than 0.05) higher than in the latter (36.4%). Correlation between endotoxaemia and specific gravity and concentrations of total protein, albumin, and globulin in ascitic fluid was studied in the group with ascites. The specific gravity of ascites in 25 patients with endotoxaemia was significantly greater than that in 17 patients without endotoxaemia (P less than 0.01). The concentration of total protein in patients with endotoxaemia (13.95 +/- 7.18 milligram, mean +/- SD) was nearly twice as high (P less than 0.01) as in patients without endotoxaemia (7.49 +/- 3.60 milligram). The protein content of those who showed reactions greater or equal to 2(+) in the Limulus assay (16.78 +/- 7.14 milligram) was a significantly (P less than 0.05) higher than in those with 1(+) reaction (11.26 +/- 6.33 milligram). Moreover, the concentration of albumin in patients with endotoxaemia (7.68 +/- 4.60 milligram) was more than twice that of the patients without endotoxaemia (3.39 +/- 1.58 milligram, P less than 0.01). On the other hand, globulin concentration in patients with endotoxaemia was 1.6 times that of patients without endotoxaemia (P less than 0.01). Similar differences were noted between endotoxaemic and non-endotoxaemic patients in the ascites-to-serum ratio in protein, albumin, and globulin. These results suggest that in liver cirrhosis endotoxaemia may cause an increase in protein concentrations in ascitic fluid, and that it may be a precipitating factor in the formation of ascites.

Adult↗
PubMed1978

Early-blocked asporogenous mutants of Bacillus subtilis are lysogenized at reduced frequency by temperate bacteriophages.

The establishment of lysogeny in early-blocked asporogenous (Spo-) mutants of Bacillus subtilis 168, which were also defective in the production of antibiotics (Abs-), by temperate phage phi105 or SPO2 was studied. It was found that the frequency of lysogenization of Spo-Abs-mutants was 10 to 20% that of the wild-type bacteria. There was no difference in the efficiency of plating and the burst size of phi105 between wild-type and mutant strains. Phi105 lysogens of mutant strains were as stable as those of the wild type. Several rifampin-resistant mutants defective in the production of antibiotics were isolated. They were also defective in spore formation and lysogenized by phi105 at reduced frequency.

Anti-Bacterial Agents↗