Biomedical subjects
Publications and source records attributed to T Ikegami.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
The distribution of T-1982, a new semisynthetic cephamycin antibiotic, was studied with whole body autoradiography in normal male mice, pregnant mice and experimental pyelonephritic mice following a single intravenous administration of 80 mg/kg of 14C-T-1982. 1. In normal male mice, the radioactivity was distributed at high concentration in the liver, kidney, lung, gastrointestinal tracts, skin, salivary gland, tongue and muscle, but was hardly observed in the central nervous system that composed of the brain and spinal cord. 2. In pregnant mice, the radioactivity was hardly observed in the fetuses. 3. In experimental pyelonephritic mice, considerable radioactivity was concentrated on the acute inflammatory area.
Absorption, distribution and excretion of sodium 7 beta-[(2R,3S)-2-(4-ethyl-2,3-dioxo-1-piperazinecarboxamido)-3-hydroxybutanamido ]-7 alpha-methoxy-3-[(1-methyl-1 H-tetrazol-5-yl) thiomethyl]-3-cephem-4-carboxylate (T-1982) were studied in rats and mice using of 14C-T-1982. 1. The binding rate of 14C-T-1982 to serum protein was about 16% in mouse, 27% in rat and 50% in human. 2. After intravenous administration to mice and rats, blood levels of radioactivity diminished rapidly. While, after subcutaneous administration to mice and intramuscular administration to rats, blood levels reached rapidly to high concentration and, declined gradually in comparison with intravenous studies. 3. Radioactivity after parenteral administration to rats and mice was distributed high into kidney, following by liver, stomach, heart, lung and ovarium, but low into brain. In new born rats, tissue levels diminished slower than that of adult rats. 4. With regard to oral administration to mice, almost all radioactivity was found in feces. This result suggested that T-1982 was hardly absorbed from gastrointestinal tracts. Urinary excretion rate after intravenous administration was about 60% in mice and 19% in rats, and the other radioactivity was found in feces. 5. In rats, radioactivity was excreted in bile at biliary excretion rate of about 80% after intravenous administration. 6. After intravenous administration to nursed rats, radioactivity was hardly detected in gastrointestinal tracts of sucklings. And from the study on distribution of radioactivity in pregnant mice, it was suggested that T-1982 scarcely passed placenta. 7. Excretion pattern after multiple administration to rats was similar to that of single administration. This result suggested that T-1982 did not produce accumulation of it in body.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
The distribution of cefoperazone (CPZ), a new semisynthetic cephalosporin antibiotic, was studied by macroautoradiography following a single intravenous or subcutaneous administration of 100 mg/kg 14C-CPZ to rats, mice, pregnant mice and experimental pyelonephritic mice. (1) In whole body autoradiograms of rats administered subcutaneously and mice administered subcutaneously and intravenously, their distribution patterns of the radioactivity were found to be similar to each case. The radioactivity was distributed at high concentration in liver, kidney, lung, tongue, salivary gland, skin, gastrointestinal tracts and retina. The radioactivity was rapidly excreted in urine and bile. And it was observed that the radioactivity excreted through bile was not reabsorbed from gastrointestinal tracts. (2) In pregnant mice administered intravenously, the radioactivity was observed the same level as whole blood level in placenta, while was hardly observed in fetuses. (3) In the experimental pyelonephritic mice administered intravenously, a considerable radioactivity was concentrated on the acute inflammatory area.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Successful long-term culture of isolated pancreatic islet as well as dissociated islet cells was accomplished with the use of Microtest II trays which provides the very small amount of culture medium that is easily conditioned. In the culture of isolated islet, the ultrastructure of the islet cells was completely preserved up to the 30th day and vigorous secretion of insulin was maintained even on the 75th day. While in the islet cell culture, insulin secretion gradually decreased from the 12th day. The latter procedure makes inoculation of a definite number of dissociated pure islet cells possible, thus opeing the way to the quantitative evaluation of B cell function in vitro.
The content of beta-hydroxyaspartic acid was measured in the urine of man and several species of animals. The configuration of urinary beta-hydroxyaspartic acid was deduced to be L-erythro in form by chromatographic comparisons with authentic samples. An increased excretion of urinary beta-hydroxyaspartic acid was observed in cats when serine or thiamine was administered with glycine. Glycine-1-14C administered to rats was incorporated into the urinary beta-hydroxyaspartic acid. The formation of beta-hydroxyaspartic acid in pig-liver homogenate increased in the presence of glutamate and thiamine pyrophosphate. These results were discussed in relation to the author's working hypothesis on the biosynthesis of beta-hydroxyaspartic acid.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.