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Biomedical subjects

T Ichikawa

Publications and source records attributed to T Ichikawa.

At least 577 records · Page 32Linked to original sources
PubMed1984

[Antitumor effect of bestatin combined with bleomycin against hepatoma AH 66 subcutaneously transplanted in rats].

Biological effect of bestatin in combination with bleomycin against rat ascites hepatoma AH 66 was investigated. The combined use of bestatin and bleomycin both at a dose of 0.5 mg/kg was found to have a strong inhibition of the tumor growth. The combined effect was also confirmed histologically as follows. In combination group, increase of necrobiosis area in the tumor lesion, augmentation of infiltration of lymphocytes and macrophages around the solid tumors, and marked replacement of necrotic area by fibrous granular lesion were stronger and more rapidly than that in the other group.

Administration, Oral↗
PubMed1984

[pm Gastric cancer and modes of invasion to the pm layer].

Two hundred and eighty-eight cases of gastric cancer, whose deepest layer of cancerous invasion was the tela muscularis propria (pm), were divided into the following 4 types according to modes of invasion to the pm layer: 1) minimal invasion type (45 cases, 16%); 2) localized invasion type (128 cases, 44%); 3) diffuse invasion type (98 cases, 34%), and 4) vascular invasion type (17 cases, 6%). This classification of modes of invasion to the pm layer was useful and reflected the gross findings of cancer, grade of vascular invasion and prognosis quite well.

Adult↗
PubMed1983

Immunochemical and immunohistochemical studies on the specificity of a monoclonal antibody to choline acetyltransferase of rat brain.

Choline acetyltransferase (ChAT) preparations partially purified from brains of rat, mouse and cat were electrophoresed in polyacrylamide gels and blotted on nitrocellulose sheets. A monoclonal antibody, Ab-57, bound specifically to one protein band which coincided with the peak of ChAT activity in each species. Using Ab-57, ChAT was demonstrated immunohistochemically in cholinergic structures in rat, mouse and cat brains. Thus, the specificity and cross-species reactivity of the monoclonal antibody Ab-57 have been established.

Animals↗
PubMed1983

Monoclonal antibodies to choline acetyltransferase of rat brain.

Monoclonal antibodies to rat brain choline acetyltransferase were produced by the hybridoma technique. Two stable cell lines, Ab-57 and Ab-60, secreted immunoglobulin of subclass IgG1. The monoclonal antibodies bound to choline acetyltransferase without blocking catalytic activity. Affinity of Ab-57 was 100-times higher than that of Ab-60. Both antibodies bound to the rat enzyme in a mutually exclusive fashion. The antibodies showed cross-species reactivity with choline acetyltransferase from several mammalian brains.

Animals↗
PubMed1983

Isolation, analysis of structure, synthesis, and biological actions of urotensin I neuropeptides.

The 41-residue neuropeptide urotensin I (UI), from the urophyses of two teleost fish species (Cyprinus carpio and Catostomus commersoni), was isolated and purified, and its amino acid sequence was determined and confirmed by synthesis of a fully active peptide. The UI peptide was found to be a close structural and biological homologue of the ovine hypothalamic corticotropin-releasing factor (CRF) and the frog skin peptide sauvagine; UI is, therefore, a phylogenetic prototype of this group of peptides. Extraction of urophyses in hot acetic or hydrochloric acid cleaves an amino terminal tripeptide yielding a fully active UI(4-41). The UI peptides are equipotent with the other two naturally occurring peptides (CRF and sauvagine) in the release of mammalian pituitary corticotropin (ACTH), but UI is several times more potent than the mammalian homologue in the stimulation of release of fish pituitary ACTH. The UI peptide and its mammalian or amphibian homologues have a long-lasting hypotensive action in mammals, via a uniquely selective vasodilatation in the superior (anterior) mesenteric vascular bed only. The significantly lower hypotensive vasodilatory action of the mammalian homologue (CRF) suggests a change in the unknown physiological role of the haemodynamic actions of the UI peptides in the mammalian gastrointestinal tract during phylogenetic progression from fishes to mammals.

Adrenocorticotropic Hormone↗