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Biomedical subjects
Publications and source records attributed to T I Ul'iankina.
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The authors have developed an immunochemical method for determining low concentrations of morphine in human serum based on using the reaction of passive hemagglutination inhibition. The method is highly sensitive and practical, does not require special equipment, expensive reagents, and preliminary treatment of the biological fluid. The method has already been used for determining the morphine level in narcomaniacs with the opium form of morphinism.
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Structural changes in the human serumal albumin under the effect of roentgen-contrast media, viz. bilignost, triiotrast, endografine, iodamide, triombrine and cardiotrast, were investigated by the method of fluorescence. The complex-formation of albumin with triiodotrast, bilignost and endografine was shown to call forth a short-wave shift of the fluorescence peak by approximately 20 nm. The binding section for these substances is characterized by an association constant equalling 1.2-10(5) M-1. The authors presume that triiodotrast, bilignost and endografine cause changes in the microstructure of the aqueo-protein layer in the area of the tryptophan residue localization. As to triombine, iodamide and cardiotrast fluorescence failed to disclose their combination with the human serumal albumin.
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