Biomedical subjects
Publications and source records attributed to T Horn.
We report on a 82-year-old woman suffering from malignant eccrine poroma on her back. The infiltrated surrounding tissue suggested lymphangiosis carcinomatosa; one axillary lymph node was enlarged. Both features, however, turned out to be only of inflammatory nature.
One hundred and twelve consecutive liver biopsies were studied by transmission electron microscopy. In 18 out of 23 biopsies from chronic alcoholics showing preserved architecture by light microscopy, basal laminas were found in acinar zone 3 applied to endothelial cells as well as to hepatocytes. Basal laminas occurred independently of Mallory bodies and lipogranulomas, and were observed even in cases where light microscopy showed no fibrosis in zone 3. In addition to basal laminas, deposition of collagen and defenestration of the endothelial cells were observed. Basal laminas were seen only rarely in biopsies from non-alcoholics. We suggest that basal lamina formation in acinar zone 3 is initiated by changes in the perisinusoidal space and that it may contribute to impaired liver cell function and possibly initiate liver cell necrosis.
The brown bowel syndrome (BBS) is a rare disease characterized by malabsorption and accumulation of lipofuscin in the smooth muscle cells of the muscularis externa of the small intestine. Recently, we incidentally observed a case of BBS in a colon operated on because of a neoplasm. Our ultrastructural investigation, which demonstrated changes in the mitochondria, further supports the significance of mitochondrial damage in BBS.
A novel combined solid-phase DNA synthesis and solid-phase DNA purification strategy is described. Removal of all N-, cap-, and phosphate protecting groups while maintaining a 5'-blocking group on the final product followed by a 5'- specific exonuclease digestion of failure sequences with the synthetic DNA still attached to the solid phase yields an essentially pure oligomer.
Explore the source record for details and available documents.
We have found large oligodeoxyribonucleotides (50-120 bases) synthesized with N,N-dialkylmethylphosphoramidites to have considerably lower cloning efficiencies than biological DNA. As evidenced by HPLC analysis, these large fragments contain as much as 30% thymidine conversion to 3-methylthymidine. If cyanoethyl- instead of methyl-phosphorous protection is employed, as anticipated, no thymidine methylation is noted. More importantly, large fragments produced by N,N-diisopropylcyanoethyl-phosphoramidite coupling show cloning efficiencies indistinguishable from biological DNA.
Explore the source record for details and available documents.
Acute pancreatitis with necrosis was followed by infection with Clostridium perfringens and formation of gas in the omental bursa. This could be seen preoperatively on an abdominal roentgenogram and was verified at operation. The patient recovered after debridement, drainage and irrigation and antimicrobial therapy.
Primary mediastinal yolk sac tumor is rare. In this report, a case of a 32-year-old male with a mediastinal yolk sac tumor was studied by light, electron, and immunofluorescent microscopy. A positive immunofluorescent reaction to alpha-fetoprotein (AFP) correlated with the PAS-positive hyaline globules and electron-dense deposits seen in intracellular and extracellular locations. Serum AFP is a useful tumor marker for monitoring the results of therapy. Review of the literature indicates that the prognosis for patients with yolk sac tumor is generally poor.
Large-cell calcifying Sertoli cell tumor (LCCSCT) is a rare histologic variant of Sertoli cell tumor. Recently we observed a case of LCCSCT of the testis with no associated endocrine abnormality. Our ultrastructural findings of rows of tight junctions, numerous intracytoplasmic filaments, and abundant rough endoplasmic reticulum in whorled formations support the Sertoli cell origin of this neoplasm.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Observations in 136 liver biopsies from patients with alcoholic and nonalcoholic liver diseases reveal that IgA deposition in liver tissues appears to have a high degree of morphologic specificity for alcohol injury. Using a direct immunofluorescence technic with fluorescein-conjugated anti-IgG, anti-IgA, anti-IgM, and anti-C1q, four different staining patterns are recognized. These are labelled as "continuous," "discontinuous," "granular," and "pericellular" types depending on their morphologic characteristics and distribution patterns. Fifty of 64 biopsies from alcoholics showed a "continuous" pattern of anti-IgA activity while only three of 72 biopsies from nonalcoholics showed a similar pattern (P less than 0.001). A "pericellular" pattern of anti-IgA activity appears to indicate a more aggressive behavior of alcoholic liver disease. "Continuous" and "pericellular" patterns are seen in "chronic active hepatitis of alcoholics" but not in chronic active hepatitis in nonalcoholics. Anti-IgM activity appears to indicate chronicity of the disease process but does not have any specificity.
The synthesis of oligothymidilic acids, (dT)m (where m = 4, 7, 10, 13, 16, 19, 22, and 25), was carried out using a solid phase approach in combination with the modified phosphotriester methodology developed in solution. Cellulose was used as the solid support after its functionalization with a specially featured dinucleoside diphosphate, 5'-0-p-chlorophenylphospho-2'(3')-0-acetyluridilyl-[2'(3')-3']-5'-0-dimethoxytritylthymidine p-chlorophenylester. The fully protected trideoxynucleoside triphosphate containing only thymidine was repeatedly used to elongate the oligonucleotide chain in the 3'-5' direction. Individual coupling yields ranged from 45% to 75%. The total time needed to prepare (dT)25 was four days. Similarly, the tridecanucleotide d(AGAAGGTACTTTT) was synthesized in good yield. The results show that this approach can be used for a fast and economic way to synthesize oligodeoxynucleotides.
A solid-phase approach using trinucleotide building blocks and functionalized cellulose as solid support was used to synthesize the 22 oligodeoxynucleotides that constitute a totally synthetic gene coding for a 43 amino acid peptide. Gastric inhibitory Polypeptide. The overall strategy used for the design and synthesis of the oligomers is described.
There were examined in situ the length, the diameter and the insertions of the 4 recti and the levator on 59 orbits, furthermore the mm. obliqui in the same way. The M. levator palpebrae superioris has an average length of 42.13 mm on the right (r) and 40,24 mm on the left (l). It is at the origin 3,93/3,54 (r/l)1 mm wide and 15.23/4.89 mm on the transitional area to its aponeurosis. The M. rectus superior is (mean) 37.31/37.02 mm long, in the middle area 8.59/7.83 mm wide and 2.32/2.20 mm thick. Its tendon in 4.29/4.26 mm long and 10.43/9.84 mm wide. The M. rectus medialis has an averaged length of 37.68/37.33 mm, a width of 9.41/9.73 mm and a thickness of 3.80/3.70 mm. Its tendon is 3.04/3.90 mm long and 10.39/9.92 mm wide. The M. rectus inferior is (mean) 36.95/37.70 mm long, 4.73/4.50 mm wide and 3.87/3.92 mm thick. Its tendon is 4.70/4.66 mm long and 8.50/8.68 mm wide. The M. lateralis has an average length of 36.36/35.94 mm, a width of 10.87/10.32 mm and a thickness of 2.87/2.54 mm. Its tendon is 7.19/7.82 mm long and 9.57/9.22 mm wide. Its longitudinal part is (mean) 37.59 mm (right) and 38.54 mm (left) long and 5.74/5.63 mm wide and 2.30/1.94 mm thick. Its oblique tendinous part has an average length of 22.12/22.93 mm, a width of 9.85/9.55 mm and a thickness of 1.50 mm (r/l). The M. obliques inferior is 31.46/30.92 mm long, 7.56/7.13 mm wide and 2.54/2.50 mm thick. The bulbus oculi has a longitudinal outer diameter of 24.57/24.8 mm and transversal diameter of 23.63/23.56 mm (mean). Its vertical diameter is 23.29/23.18 mm. The transversal diameter of the cornea is 11.70/12.08 mm, the vertical diameter 10.80/11,06 mm. Besides of these measurements the distances were estimated between the muscle-tendons and the cornea as well as the optic nerve.
Explore the source record for details and available documents.
Explore the source record for details and available documents.