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Biomedical subjects

T Horiuchi

Publications and source records attributed to T Horiuchi.

At least 811 records · Page 45Linked to original sources
PubMedDate not supplied

Surgical treatment for isolated hypoplasia of the right ventricle.

Four patients with isolated hypoplasia of the right ventricle who underwent intracardiac repair are described. An atrial septal defect (ASD) was successfully closed in 3 patients with mild hypoplasia of the right ventricle. The remaining patients underwent ASD closure and right atrium-to-pulmonary artery connection with a conduit with a bovine pericardial valve because of severe hypoplasia of the right ventricle and tricuspid valve. Postoperative angiocardiogram in the last patient demonstrated two-channel pathways from the right atrium to the pulmonary artery via both the right ventricle and external conduit. At long-term follow-up studies, 3 patients had developed arrhythmias and/or sinus node dysfunction, and one had died, probably because of arrhythmia, 13 years postoperatively. The right ventricular ejection fractions were below normal levels at 11 and 12 years postoperatively in 2 patients who underwent closure of ASD. It was concluded that the mild form of hypoplasia of the right ventricle should be surgically corrected by ASD closure, while right atrium-to-pulmonary artery connection or Fontan's operation should be chosen for cases of severe hypoplasia. Arrhythmias and hypofunction of the right ventricle are long-term problems after intracardiac repair of this anomaly.

Adolescent↗
PubMedDate not supplied

Measurement of peritoneal dialysate volume by CT.

Three-dimensional (3-D) graphic reconstruction of serial image data of x-ray CT on three dialysis patients was studied to measure peritoneal dialysate (PD) volume. Volume estimation with a surface reconstructed model provided a 16% error; a 35% error was found with a voxel model, although the total time required by the voxel model was 15 times less.

Aged↗
PubMedDate not supplied

Detection of fowlpox virus DNA by in situ hybridization using a biotinylated probe.

In situ hybridization was applied to detect fowlpox virus (FPV) DNA in formalin-fixed paraffin-embedded sections of the skin from infected chickens by using a biotinylated probe and a streptavidin-alkalinephosphatase conjugate. The immunohistochemical examination was applied to compare the distribution of the FPV DNA to that of related antigenic protein in serial sections. In the infected epithelial cells, FPV DNA was detected in cytoplasmic inclusion bodies and in the rest of cytoplasm. Likewise, immunohistochemical examination revealed the virus antigen in cytoplasm. Ultrastructurally, virions were observed in the cytoplasmic inclusion bodies, and immature virus particles were in the rest of the cytoplasm. The study proved restricted distribution of FPV DNA in the cytoplasm.

Animals↗
PubMedDate not supplied

Pathological changes in chicks inoculated with the picornavirus "avian nephritis virus".

One-day-old chicks were inoculated intraperitoneally with a newly isolated picornavirus. The inoculated chicks showed no clinical signs until 28 days postinoculation (PI), but discoloration of the kidneys was recognized from 7 to 21 days PI at autopsy. Histologically, focal lesions were observed in the cortex of the kidneys from 3 to 21 days PI. The lesions were characterized by interstitial lymphocytic infiltration and degeneration of epithelial cells of the proximal convoluted tubules. The degenerated cells contained acidophilic granules in their cytoplasm. Electron-microscope examination of the cytoplasm revealed electron-dense amorphous areas, phagosomal areas with viral particles, and isolated crystal arrays of the virus particles, 23 to 30 nm in size. Granular antigen was also detected by fluorescent-antibody technique in the cells.

Animals↗
PubMedDate not supplied

Pathologic changes in chickens caused by intravenous inoculation with fowlpox virus.

Twenty chickens were inoculated intravenously with fowlpox (FP) virus, and clinical and pathological examinations were carried out chronologically. Upon gross examination, miliary nodules scattered in the kidneys were observed from 10 to 18 days postinoculation (PI), as were papules on the skin and diphtheritic lesions on the mucous membrane of the upper respiratory tract. Microscopically, characteristic FP lesions, composed of swelling and proliferation of cells with formation of Bollinger bodies, were observed in the epithelial cells of renal tubules from 4 to 14 days PI and in the epithelial reticular cells of the thymic medulla from 4 to 10 days PI, as well as in the skin and mucous membrane. Immunofluorescent and electron microscopic observations confirmed the presence of viral antigen and virus particles in the characteristic lesions of FP.

Animals↗
PubMedDate not supplied

Pathological changes of tracheal mucosa in chickens infected with lentogenic Newcastle disease virus.

Forty-day-old specific-pathogen-free chickens were exposed by aerosol to lentogenic Newcastle disease virus (NDV) and observed for 24 days for pathological changes in the tracheal mucociliary system. Specific fluorescence of NDV antigen was observed through day 5 postexposure (PE) in the cytoplasm of the tracheal epithelium and desquamated epithelium in the lumen. On day 1 PE, scanning electron microscopy revealed hypertrophy of goblet cells and small patches of the deciliated epithelium scattered mainly around the openings of mucous glands. The deciliated area of tracheal surface increased through day 4 PE. Light microscopy showed small vacuoles containing lymphocytes and heterophils in the epithelial layer. Immature epithelium proliferated in some areas. On days 5 and 6 PE, ciliated areas of the trachea tended to increase as a result of regeneration of the epithelium, still leaving many nonciliated patches of various sizes. On and after day 8 PE, there remained plaques with nonciliated flat epithelium, but most areas were covered with well-ciliated epithelium. Non-ciliated plaques were observed until day 24 PE, but they gradually decreased in size. These plaques were covered by a single layer of flat epithelium and were formed upon lymph follicles in subepithelial tissue.

Animals↗
PubMedDate not supplied

Pathological changes of tracheal mucosa in chickens infected with infectious laryngotracheitis virus.

Six-week-old chickens were inoculated via the posterior thoracic air sac with infectious laryngotracheitis virus. Chickens were sacrificed on various days through day 16 postinoculation (PI), and the trachea was examined by scanning electron microscopy (SEM) and light microscopy (LM). The pathological changes observed on day 1 PI were hypertrophy and hyperplasia of goblet cells. From day 3 PI, the epithelial cells protruded collectively and fused to form syncytia, which contained many intranuclear inclusion bodies. Subsequently, epithelial syncytia desquamated, one after another, and connective tissues were exposed in places. Serofibrinous exudate and detritus were abundant on the surface of the exposed connective tissues and seemed to form a pseudomembrane. On day 5 PI, the remaining epithelial cells began to repair the devastated mucosa just under the pseudomembrane. On day 6 PI, microvillus-rich regenerating epithelial cells were arranged like paving stones. On day 8 PI, the epithelial cells proliferated extensively and formed folds with cyst-like structures. By day 16 PI, the tracheal epithelium was covered with cilia and regained its normal histologic appearance.

Animals↗
PubMedDate not supplied

Real time sound spectral analysis for diagnosis of thrombosed prosthetic valves.

A real time sound spectral analyzer was developed at the authors' institute and has been used for diagnosis of thrombosed valves for 15 years. The analyzer consists of a wider frequency microphone, a digital signal processor with multiple bandpass filters, and displays. To quantify the high frequency component in section pattern of opening and closing clicks, maximal frequency at -30 dB level was used at a diagnostic parameter (normalized maximal frequency, NMF). A total of 17 thrombosed valves with or without pannus formations have been experienced and SSA showed abnormality in 13 valves, which comprised of Björk-Shiley (N = 6), Starr-Edwards 2320, 6120, 6520 (N = 5) and Kay-Shiley (N = 2). The abnormal findings included NMF of opening or closing clicks lower than normal range of each type of prosthesis, and significant decrease of NMF more than 450 Hz from the value previously obtained. All diagnoses of thrombus formations and their locations were confirmed by operative or autopsy findings. The SSA failed to diagnose in four valves, which comprised of Starr-Edwards 6520 (N = 3) and Björk-Shiley (N = 1). Operative findings revealed that thrombi were formed in a manner that permitted direct collision of disc and valvular ring causing clicks with high frequency component. No pseudo-positive diagnosis for thrombosed valve was experienced in the authors series, and SSA is thought to be a reliable noninvasive technique that enables early diagnosis of a thrombosed valve.

Adult↗
PubMedDate not supplied

Selective removal of high level cryoglobulins (CG) and immune complexes (IC) by a large pore membrane.

The investigated membranes having a pore size of 3 micron or less can retain over 95% of cryoprecipitable proteins and immune complexes with nearly complete passage of other plasma proteins at 4 degrees C. Based on this result, the G-3 module consisting of V-3000 membrane was chosen for further study. The G-3 module (0.148 m2 of surface area) processed a 10 times higher plasma volume per unit surface area with higher selectivity than the Asahi Plasmaflo AP 06M. Separation efficiency of cryoprecipitable proteins and immune complexes in cryoglobulinemic plasmas by cryofiltration at 4 degrees C is related to the composition of the cryoprecipitable protein; three of four plasmas contained cryoprecipitable proteins that were removable by cryofiltration. Cryoprecipitation was too slow in one of four plasmas. For this plasma SDS PAGE showed its light chain to be of higher molecular weight than other plasmas. Two of four plasmas had elevated amounts of IC that were removed by cryofiltration.

Adult↗
PubMedDate not supplied

Successful surgical treatment of pulmonary artery sling and partial anomalous pulmonary venous drainage in an infant.

A 8 month old infant with pulmonary artery sling and partial anomalous pulmonary venous drainage was reported. The patient was seen initially with severe stridor. Diagnosis was made by barium swallow study, echocardiography, bronchoscopy, and pulmonary arteriography. The patient was operated on through a median sternotomy; the left pulmonary artery was dissected deep between the trachea and esophagus, and then reimplanted to the side of the main pulmonary artery. Systemic heparinization and microsurgical technique were used. Simultaneous intracardiac repair was carried out by means of extracorporeal circulation. The patient improved dramatically after operation. Postoperative pulmonary arteriogram revealed a wide patent left pulmonary artery, and postoperative perfusion lung scan confirmed improved perfusion of the left lung. It is concluded that pulmonary artery sling should be treated surgically as soon as diagnosed. From the surgical point of view, the left pulmonary artery should be anastomosed to the main pulmonary artery with micro-surgical technique to avoid late occlusion of the left pulmonary artery.

Abnormalities, Multiple↗
PubMedDate not supplied

Leakage across the premoulded dacron baffle used for correction of transposition of the great arteries.

The use of a pre-moulded dacron baffle, as described by Dillard, and used as an interatrial baffle in transposition of the great arteries resulted in an extensive leakage of blood through the baffle for several days. Pre-clotting of the baffle on one occasion only was ineffective in preventing the leakage. But complete sealing was effected by pre-clotting three times according to the method of Sauvage. These clinical findings were confirmed by microscopic analysis. A porous baffle way will be an advantage in patients with pulmonary hypertension when it would act as a safety valve.

Angiocardiography↗
PubMedDate not supplied

Valve replacement of childhood: follow-up results and complications.

This study concerned 18 cases of value replacement in patients than 15 years, between 1964 and 1980. In this period, there was a total of 30 such cases, but there were eight hospital deaths and four cases with less than two years postoperative observation. These 12 cases were excluded from the study. The average follow-up period of the 18 cases was eight years and nine months. During this period, six cases were associated with postoperative complications, which consisted of three cerebral emboli, two malfunctions of the implanted prostheses and one congestive heart failure. Three cases involved prosthesis-related death. Secondary implantations were performed in two cases; one for relative stenosis of the prosthesis and the other for calcification of the porcine xenograft. Physical activity in the 15 survivors has been excellent in all but two cases with arrhythmia and sequelae of cerebral damage due to surgery.

Adolescent↗