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Biomedical subjects

T Horiuchi

Publications and source records attributed to T Horiuchi.

At least 631 records · Page 35Linked to original sources
PubMed1978

Development of resistance to hepatotoxic effect of furylfuramide by pretreatment with its subnecrotic doses and carbon tetrachloride.

Hepatotoxicity caused by furylfuramide in ddYS male mice was further investigated with following results. (1) Pretreatment with successive oral administration of non-toxic (non-necrotizing) dose (100 mg/day) of furylfuramide for 8 or 24 days alleviated the liver damage elicited by a necrotizing dose (250 mg/kg). The pretreatment resulted in the significant enhancement of the activity of aniline hydroxylase in the liver microsome, which was reduced markedly by a single administration of 250 mg/kg (without pretreatment). The result suggests that the acquired resistance to a toxic dose of furylfuramide is due to the acceleration of detoxication of the drug. (2) Simultaneous administration of CCl4, another hepatotoxic substance, with furylfuramide reduced the lethal effect of the latter. The LD50 values of furylfuramide were increased more than 2.4 times as compared with that of furylfuramide alone. Pretreatment with a small amount of CCl4 as short period as half an hour is more effective than simultaneous administration of both substances. This inhibitory effect of CCl4 on furylfuramide may be explained by an inhibition of the metabolic activation of the latter by the former.

Alanine Transaminase↗
PubMed1977

Purification and properties of a lambda operator-binding protein which is expected to be autorepressor (tof protein) from E. coli carrying lambdadv plasmid.

In order to study the mode of action of the tof gene product, which is an "autorepressor" of the bacteriophage lambda and plasmid lambdadv, we have purified a DNA-binding protein which is specifically produced in bacteria carrying lambdadv. This protein possesses characteristics expected for the product of the tof gene, since it is produced under conditions where cI-repressor is not made, and since it binds to oL and oR operators on the lambda phage genome. The molecular weight of the native protein is 16,000-17,000 daltons, and the monomeric molecular weight as measured by gel electrophoresis in the presence of sodium dodecyl sulfate is about 10,000 daltons. Denaturation and renaturation experiments demonstrated that the native protein is a dimer of 10,000-dalton monomers. The lambdaDNA-specific binding protein is not produced in cells carrying i21dv or ø80dv.

Bacterial Proteins↗
PubMed1977

Purification and some properties of presumptive tof gene product of Coli phage 434.

The presumptive tof gene product of Coli phage 434 has been purified from cells carrying lambdaimm434cIdv plasmid known to contain only some of the "early" genes of phage 434 and lambda. It was detected and tentatively identified as tof protein primarily by its ability to specifically bind to phage 434 DNA. The protein has a molecular weight of about 11,000 and requires Mg2+ for specific DNA binding, unlike 434 cI-repressor.

Coliphages↗
PubMed1977

Pathological changes in chickens inoculated with reticuloendotheliosis-virus-contaminated Marek's disease vaccine.

A disease characterized by delayed growth, anemia, abnormal feathers, and leg paralysis occurred among chickens inoculated with Marek's disease vaccine over a period from spring to fall in 1974. These chickens were recognized among flocks inoculated with the vaccine produced by two vaccine makers. The affected ones were examined pathologically. Gross examination revealed a slight enlargement of peripheral nerves and atrophy of the spleen, thymus, and bursa of Fabricius. Histopathologically, the peripheral nerves had a mild cell infiltration of lymphoid and plasma cells, edema, degeneration of nerve fibers with Schwann's cell proliferation. Perivascular cuffings consisting mainly of lymphoid cells were seen in the brain and spinal cord. Atrophic changes displayed by prominent reduction of lymphocytes were recognized in the spleen, thymus, and bursa of Fabricius. Etiological examination suggested that most of the chickens examined might have been infected with reticuloendotheliosis virus and not with Marek's disease virus. The pathological changes observed in the peripheral nerves and central nervous system, however, were not distinguishable from those of Marek's disease.

Animals↗