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Biomedical subjects

T Horio

Publications and source records attributed to T Horio.

At least 289 records · Page 16Linked to original sources
PubMed1981

Evaluation of drug phototoxicity by photosensitization of Trichophyton mentagrophytes.

Long wave ultraviolet radiation to suspensions of T. mentagrophytes, in the presence of photosensitizing chemicals, resulted in death of the fungus as measured by loss in colony-forming ability. Photosensitization could be demonstrated, with appropriate exposures and concentrations, with chlorpromazine, promethazine, perphenazine, anthracene, sulphanilamide, trimethylpsoralen, tolbutamide, tetrachlorosalicylanilide, and tribromosalicylanilide. No phototoxicity was detected with demethylchlortetracycline and thiazides. The present system seems to be simple, reproducible, and sensitive as an in vitro screening test for the photosensitizing potential of chemicals.

Drug Evaluation, Preclinical↗
PubMed1981

Colchicine in the treatment of the cutaneous manifestations of Behçet's disease.

Five patients with Behçet's disease were satisfactorily treated with colchicine. Oral aphthosis, erythema nodosum-like lesions and genital erosions improved greatly within a month as did laboratory findings. We believe colchicine to be the first choice in the management of the cutaneous and ocular lesions of Behçet's disease.

Adult↗
PubMed1981

Photosensitization of Trichophyton with 8-methoxypsoralen to UV-A light radiation.

The photosensitization effect of 8-methoxypsoralen (8-MOP) and subsequent long-wavelength ultraviolet(UV-A) irradiation on Trichophyton mentagrophytes was examined by in vitro, semi-in vivo, and in vivo experiments. A marked fungicidal effect of 8-MOP plus UV-A (PUVA) on suspensions of fungal spores was observed in vitro as measured by loss in colony-forming ability of T. mentagrophytes. 8-MOP application or UV-A irradiation alone did not show such an ability. The antifungal effect of PUVA was also observed on the infected scales obtained from experimental and clinical dermatophytosis. The in vivo activity of PUVA treatments was also demonstrated on the lesions of experimentally induced dermatophytosis in guinea-pigs. These observations suggest possible clinical applications of photochemotherapy on superficial fungal infections.

Animals↗
PubMed1981

Sulphhydryl and disulphide stainings of subepidermal hyaline bodies.

The localization of sulphhydryl (-SH) groups and disulphide (-S-S-) bonds in subepidermal hyaline bodies was examined using histochemical techniques with the specific fluorescent thiol reagent (DACM). In lichen planus, hyaline bodies showed -S-S- but not -SH staining, suggesting that erythematosus and dermatomyositis, there were not only -S-S-positive hyaline bodies, considered of epidermal origin, but also -S-S- negative ones. The latter may consist of dermal components, since no dermal components are stainable with DACM. DACM staining therefore seems to be of help in determining the origin of hyaline bodies.

Dermatomyositis↗
PubMed1981

Sulphhydryl and disulphide stainings of amyloid: a comparison with hyaline body.

Histochemical staining for sulphhydryl (-SH) groups and disulphide (S-S) bonds was undertaken in 10 cases of cutaneous amyloidosis using the new fluorescent thiol reagent. A weak S-S fluorescence (but not -SH) was detected in dermal amyloid, suggesting that cross-linking of -SH groups may occur in amyloidogenesis. Since in normal skin S-S bonds were stained only in the horny layer and negligibly in the dermis, our findings seem to support the view that amyloid is derived at least partially from epidermal cells.

Amyloid↗
PubMed1981

Tumor-associated serum proteins in rats as detected by two-dimensional gel electrophoresis.

The sera from normal and tumor-bearing rats were separated by polyacrylamide gel disc isoelectric electrophoresis and then in a perpendicular dimension by polyacrylamide gel slab electrophoresis with a gradient from 4% to 20%. When the gel was stained with Coomassie brilliant blue, 100-150 components were separately observable with normal serum. With the sera from Rhodamine sarcoma-bearing rats, three more components were detected with a high reproducibility. They were of pI (isoelectric point) 4.5 and Rm (relative mobility) 0.63, of pI4.8 and Rm 0.63 and of pI 4.9 and Rm 0.75. The 4.5/0.63 (pI/Rm) and the 4.8/0.63 components appeared soon after transplanting the tumor, whereas the 4.9/0.75 component appeared at the late stage of its growth. Upon surgical removal of the grown tumor, the former two components disappeared rapidly, while the latter component disappeared gradually. Similar results were obtained with Yoshida sarcoma, and ascites hepatoma AH66 and AH130. A 3rd polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate revealed that the 4.5/0.63 and the 4.8/0.63 components were each composed of a single polypeptide of approximately 64,000 daltons, and that the 4.9/0.75 component was composed of two different polypeptides of approximately 33,000 daltons and approximately 10,000 daltons. It was confirmed by an immunodiffusion experiment that none of these three proteins was alpha-fetoprotein.

Animals↗
PubMed1981

Potassium iodide in the treatment of erythema nodosum and nodular vasculitis.

Twenty-nine patients with erythema nodosum, nodular vasculitis, or erythema nodosum-like lesions associated with Behçet's syndrome were treated with potassium iodide. Administration of the drug for systemic effect showed a substantial effect in 11 of 15 patients with erythema nodosum, seven of ten with nodular vasculitis, and one of four with leg lesions of Behçet's syndrome. Relief of subjective symptoms, including tenderness, joint pain, and fever, occurred within 24 hours. Substantial improvement in the eruption occurred within a few days, and the lesions disappeared completely ten to 14 days after therapy was initiated. The patients to whom the medication was administered shortly after the initial onset of erythema nodosum seemed to respond most satisfactorily. The effect of the drug was marked in the patients with positive C-reactive protein reactions, joint pains, and/or fever. Possible mechanisms by which potassium iodide exerts its effect are discussed.

Adult↗
PubMed1980

Chromatography in presence of high concentrations of salts on columns of celluloses with and without ion exchange groups (hydrogen bond chromatography). Its application to purification of yeast enzymes.

1. All the water-soluble yeast enzymes tested, which were only partially precipitated at best in the presence of high concentration of salts such as ammonium sulfate or sodium formate, were adsorbed on a column of cellulose in the presence of the same concentrations of the salts, and the adsorbed enzymes were chromatographically eluted by decreasing the concentration of the salts. 2. Even in the presence of high concentration of the salts, the adsorbed enzymes were eluted by urea or by "hydroxy-rich" reagents such as sucrose. 3. Under the experimental conditions used, the salt concentrations required for elution of the adsorbed enzymes were lower with cellulose than with DEAE-cellulose, CM-cellulose, or P-cellulose, indicating that ion exchange groups, either cationic or anionic, affected the adsorption, although the ion exchange groups of DEAE-cellulose, CM-cellulose, and P-cellulose were weakly but definitely functional as ion exchangers even in the presence of high concentrations of the salts. 4. The principal attractive force between cellulose and the enzyme was deduced to be due to hydrogen bonding. 5. This hydrogen bond chromatography was applied for the purification of some yeast enzymes.

Alcohol Oxidoreductases↗
PubMed1980

Affinity chromatography of porcine pancreas deoxyribonuclease I on DNA-binding sepharose under non-digestive conditions, using its substrate-binding site.

1. DNase I from porcine pancreas, if Mg2+ was present, hydrolyzed both sDNA and dDNA, whether free or bound to Sepharose. The hydrolysis rates were maximum at pH 7.5 with the bound DNAs and at pH 7.0 with the free DNAs negligible at pH 4.0 and pH 10.5 with the free and bound DNAs. The hydrolysis was completely inhibited by 50 mM sodium citrate. 2. With 50 mM citrate buffer (Ph 4.0), DNase I was effectively adsorbed on the DNA-Sepharoses in the absence of 5 mM Mg2+. The adsorbed enzyme was effectively eluated by the buffer containing 1 M KCl (eluate). The amounts of the eluated enzyme were approximately 1.5 X 10(5) units/mg DNA with sDNA-Sepharose and approximately 3.0 X 10(5) units/mg DNA with dDNA-Sepharose. This simple adsorption-elution of the pancreas extract resulted in approximately 300-fold purification of DNase I with a yield of 95%. In the elute, the ratios in activity of trypsin, chymotrypsin and RNase to DNase I were 1/(4.0 X 10(5)), 1/(5.3 X 10(3)), and 1/(4.1 X 10(2)) as low as in the extract, respectively. In addition, the eluate was not contaminated by kallikrein or carboxypeptidases A and B. 3. Upon repeating the adsorption-elution described above, the adsorbing capacities of DNA-Sepharoses gradually deteriorated with the whole pancreas extract, but not with the precipitate of the extract formed on 60% ammonium sulfate saturation, which contained 90% of the DNase I. With the precipitate, one dDNA-Sepharose solumn was repeatedly usable at least 20-times without deterioration. The DNase I preparation thus obtained was homogeneous on SDS-polyacrylamide gel electrophoresis. 4. Conceivably, the above-mentioned adsorption of DNase I on DNA-Sepharoses was mainly due to the steric and electrostatic affinity of a relatively large moiety of the DNA molecule to the substrate-binding site, but not to the catalytic site, of the enzyme.

Animals↗
PubMed1980

Benign and neoplastic eosinophilic staining cells: an immunofluorescence study.

Deposition of immunoglobulins, complement and fibrinogen on eosinophilic staining cells was investigated using direct immunofluorescence techniques. Serum factor deposition was detected on benign epidermal eosinophilic cells seen in pityriasis lichenoides et varioliformis acuta, sunburn erythema and, in addition, on subepidermal hyaline bodies in lichen planus; no such deposition occurred on neoplastic eosinophilic cells in Bowen's disease and squamous cell carcinoma. The qualitative findings of immunofluorescence microscopy seem to be different in inflammatory and malignant dermatoses.

Complement System Proteins↗
PubMed1980

Histochemical staining of sunburn cells for sulphhydryl and disulphide groups: a time course study.

Ultraviolet light (UV)-exposed rabbit skin was stained with a fluorescent thiol reagent or haematoxylin and eosin to investigate a time relationship between histochemical stainings for sulphhydryl (-SH) and disulphide (S-S) groups and sunburn cell (SBC) production. SBCs appeared by 8 h after irradiation, increased and showed a peak at 48 h, then declined to zero by 96 h. SBCs showing -SH staining increased to a maximum of 41--59% at 24--48 h and declined to 26--45% by 72 h, whereas S-S-stained SBCs were only a few at 8--24 h and then increased up to 65--85% by 72 h. The results seem to indicate that S-S linkage may occur in the process of SBC production.

Animals↗
PubMed1980

Treatment of acute febrile neutrophilic dermatosis (Sweet's Syndrome) with potassium iodide.

6 cases with acute febrile neutrophilic dermatosis (Sweet's syndrome) responded rapidly and dramatically to treatment with potassium iodide. All patients became afebrile and symptom-free within 24--48 h after therapy. The cutaneous eruptions subsided completely in 3--5 days. 5 of the patients received the drug only for 2 weeks, but they have had no recurrences. Although the remaining 1 patient had shown minor recurrences after cessation of the medication, he was free of all symptoms during the therapy. The mode of the action of potassium iodide is also discussed.

Adult↗