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Biomedical subjects

T Horii

Publications and source records attributed to T Horii.

At least 163 records · Page 9Linked to original sources
PubMed1986

[Fundamental and clinical studies of imipenem/cilastatin sodium in the field of obstetrics and gynecology].

Fundamental and clinical studies of imipenem/cilastatin sodium (MK-0787/MK-0791), a new carbapenem antibiotic, were carried out. The results obtained were summarized below. At a dose of 500 mg/500 mg, the penetration of MK-0787/MK-0791 into internal genital organs was good. The penetration of MK-0787/MK-0791 into pelvic dead space exudate was also good. Clinical results obtained were good in 4 patients and poor in 1, with an efficacy rate of 80%. No side effects were observed, but 1 patient showed slight elevations of GOT, GPT, Al-P and LDH. From these results, MK-0787/MK-0791 appeared to be a useful drug for the treatment of various infections in the field of obstetrics and gynecology.

Adult↗
PubMed1985

Structural analysis of the umu operon required for inducible mutagenesis in Escherichia coli.

The nucleotide sequence of a 2.8-kilobase fragment of the Escherichia coli chromosome containing the umuDC genes has been determined. The DNA sequence specifies two open reading frames of 417 and 1266 nucleotides encoding proteins with calculated molecular weights of 15,063 and 47,677, respectively. From these and the previous results of genetic and biochemical studies on the cloned genes, we conclude that the former is the umuD and the latter is the umuC gene. In vitro transcription of the regulatory region of the umu operon revealed that (i) upstream of the coding region there is a promoter-operator complex having the consensus sequence, CTGTATATAAAAACAG, of an SOS box, (ii) transcription of the umu operon begins at an adenine residue in the SOS box, and (iii) the LexA protein binds to the operator region, with an equilibrium dissociation constant (Kd) of 0.2, thereby repressing the transcription of the umuDC genes. These results indicate that the expression of the umu operon is under the coordinated control of the recA lexA gene products.

Amino Acid Sequence↗
PubMed1985

[Fundamental and clinical studies on aztreonam in the field of obstetrics and gynecology].

Fundamental and clinical studies were performed on aztreonam (AZT), a new antibiotic with single beta-lactam ring, with the following results. Following intravenous drip infusion of 1 g, transfer of AZT to the internal genital organs was found to be good. Transfer of AZT to exudate of pelvic dead space was also good. AZT was given to 6 cases, who had gynecological or obstetrical infectious disease. AZT was effective in the 5 cases out of 6, although it showed high MIC against bacteria isolated from the effective cases. The above results demonstrated that AZT was a safe and effective drug.

Adult↗
PubMed1985

[Basic and clinical studies on cefminox in the field of obstetrics and gynecology].

Basic and clinical studies were carried out on cefminox (CMNX, MT-141), a new cephem antibiotic. Results obtained were as follows. Following each 1 g of intravenous drip infusion and intravenous injection, transfer of CMNX to the internal genital organs was good. Transfer of CMNX into exudate of the pelvic dead space was also good and showed high concentration. CMNX was given to 2 cases. Clinical efficacy was good in 1 case, and could not be evaluated in 1 case. No side effects were observed in both cases. The above results show that CMNX is an effective agent.

Adult↗
PubMed1985

[Fundamental and clinical studies on cefpimizole in the field of obstetrics and gynecology].

The findings from fundamental and clinical studies on cefpimizole (AC-1370) in the treatment of infections in obstetrics and gynecology are reported. We investigated the transfer of AC-1370 into female genital tissues of 6 patients and the transfer of the drug into the pelvic dead space exudate in 9 patients by means of determining its concentrations in the tissues 90 to 210 minutes after the end of intravenous drip infusion of the drug over 1 hour. The mean concentration was 13.06 micrograms/g for the oviduct, 16.50 micrograms/g for the ovary, 16.50 micrograms/g for the endometrium, 14.92 micrograms/g for the myometrium, 16.58 micrograms/g for the uterine cervix, 17.75 micrograms/g for the portio vaginalis, 10.46 micrograms/ml for the uterine arterial serum and 9.91 micrograms/ml for the antecubital venous serum. The drug proved to be well transferred into the pelvic dead space exudate; it reached a peak of mean 2 hours after the end of the intravenous drip infusion of the drug over 1 hour, then decreased steadily, and was found to be a mean of 16.7 micrograms/ml even 8 hours after the end of the intravenous drip infusion. Four patients with infections in obstetrics and gynecology were then treated with AC-1370. Three out of the 4 patients responded to the treatment, while the response of another patient was unknown. None of the 4 patients showed any side effects of the drug or any abnormalities in laboratory findings.

Adult↗
PubMed1984

Molecular cloning of cDNA for rat glycine methyltransferase.

Using a highly purified preparation of glycine methyltransferase mRNA, double-stranded cDNA was synthesized and inserted into the PstI site of pBR322. The resulting recombinant DNA was used to transform E. coli X 1776 by conventional methods. Among tetracycline-resistant transformants, a number of colonies were found to contain cDNA sequence for glycine methyltransferase as examined by hybrid-selected translation. A restriction endonuclease cleavage map was constructed covering about 720 base pairs. With the cDNA as the probe, the content of the glycine methyltransferase mRNA was quantitated in various rat tissues and was found to be proportional to the specific enzyme activity.

Animals↗
PubMed1984

Cysteinyl residues of Escherichia coli recA protein.

The Escherichia coli recA protein has three cysteinyl residues at positions 90, 116, and 129. All of them are reactive with 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB). In the presence of ATP or ADP, only one cysteinyl residue reacts with DTNB. The residue was also reactive with N-[7-(dimethylamino)-4-methylcoumarinyl]maleimide (DACM) in the presence of ATP. The results on an analysis of the DACM-modified protein cleaved at the nonmodified cysteinyl residues after cyanation with 2-nitro-5-(thiocyano)benzoic acid show that two cysteinyl residues protected in the presence of ATP or ADP are identified as Cys-90 and Cys-129. When the ionic strength is higher than 1, one cysteinyl residue does not react with DTNB. This residue is Cys-90 or Cys-129, because one of the two cysteinyl residues, which are not modified with DACM in the presence of ATP, does not react with DTNB at high ionic strength. The binding of single-stranded DNA to the recA protein does not change the reactivity of the cysteinyl residues with DTNB.

Amino Acids↗
PubMed1984

Functional domains of Escherichia coli recA protein deduced from the mutational sites in the gene.

The sites of recA mutations of Escherichia coli, recA441 (tif-1), recA1, recA430 (lexB30) and recA44, were determined by analyses of the nucleotide sequences. All mutations are single point missense mutations within the coding region of the recA gene. In the recA441, recA1, recA430 and recA44 proteins, the 38th, 160th, 204th, and 246th amino acids, respectively, from the amino terminal ends are altered. Based on the properties of these mutant proteins and modified forms of recA protein, the locations of various regions of the recA protein that are involved in binding with ATP, binding with single-stranded DNA, hydrolysis of ATP, interaction between the recA protein molecules and interaction with the lambda cI or lexA repressors are mapped on the primary structure of the protein.

Amino Acid Sequence↗
PubMed1984

[Clinical studies on sulbactam/cefoperazone in the field of obstetrics and gynecology].

Sulbactam (SBT), a new beta-lactamase inhibitor, in combination with cefoperazone (CPZ) was studied for the clinical efficacy in the field of obstetrics and gynecology. SBT/CPZ was given to 5 cases with the following infections; 2 of pyometra, 2 of endometritis and 1 of abscess of adnexa. Clinical efficacy was good in 3 cases and poor in 2 cases. Bacteriologically, only 1 strain of P. vulgaris was beta-lactamase producer but was persisted after treatment. Neither side effects nor abnormal laboratory findings was observed.

Adult↗
PubMed1984

[Fundamental and clinical studies of ceftriaxone in the field of obstetrics and gynecology].

Fundamental and clinical studies on ceftriaxone (Ro 13-9904, CTRX), a new cephem antibiotic, were carried out with the following results. Following each 1.0 g of drip infusion, transfer of CTRX to female genital organs was found to be excellent. Transfer of CTRX to exudate of the pelvic dead space was also excellent. And high concentration of CTRX was kept for long time after administration. CTRX was given to 7 cases. It was effective for 5 cases and ineffective for 2 cases. The above results demonstrated that CTRX is a safe and effective drug.

Adult↗
PubMed1984

[Fundamental and clinical studies on ceftazidime in the field of obstetrics and gynecology].

Fundamental and clinical studies were carried out on ceftazidime ( CAZ ), a new cephalosporin antibiotic, with the following results. Following each 1.0 g of drip infusion and bolus intravenous injection, transfer of CAZ to the internal genital organs was found to be good. Transfer of CAZ into exudate of the pelvic dead space was also good. CAZ was given to 6 cases. Clinical efficacy was good in 5 cases and poor in 1 case. No side effects were observed in any case. The above results demonstrated that CAZ is a safe and effective drug.

Adolescent↗
PubMed1981

Regulation of SOS functions: purification of E. coli LexA protein and determination of its specific site cleaved by the RecA protein.

The LexA protein of Escherichia coli was purified to more than 96% purity from cells harboring a recombinant plasmid carrying the lexA gene with the lacZ promoter sequence. The amino acid composition of the LexA protein and its amino-terminal sequence were analyzed. The results are in agreement with the prediction from the nucleotide sequence of the lexA gene. The LexA protein is cleaved into two polypeptides by E. coli RecA protein in the presence of ATP and single-stranded DNA. The site of the specific cleavage was determined by analyzing amino acid sequences of the cleaved products at the amino and carboxyl termini. The cleavage of the LexA protein by the RecA protein was found to occur at a single site between Ala84 and Gly85.

Bacterial Proteins↗
PubMed1981

Nucleotide sequence of the lexA gene of E. coli.

Using a cloned fragment containing the lexA gene of E. coli, the entire nucleotide sequence of the lexA gene has been determined. The probable coding region of the lexA gene contains 606 nucleotide residues and encodes a single protein of 202 amino acids. The initiation site of in vitro transcription of the lexA messenger RNA has been determined by analysis of the 5' nucleotide sequence. Comparison of the DNA sequence of the promoter region of the lexA gene with that of the recA gene reveals the presence of sequences that are common to both. There is some similarity between the amino acid sequences of the lexA and the lambda repressor proteins.

Bacterial Proteins↗
PubMed1980

Organization of the recA gene of Escherichia coli.

The restriction map of a BamHI DNA fragment that contains the recA gene of Escherichia coli has been established and a large portion of the fragment's nucleotide sequence has been determined. The coding region of the recA gene contains 1059 nucleotide residues and encodes a single protein of 353 amino acid residues. The amino acid sequence of the first five residues of the NH2 terminus of the recA protein agrees with the sequence predicted from the DNA sequence except for the absence of formylmethionine in the purified protein. Immediately after the coding sequence, there is a G+C-rich sequence with dyad symmetry followed by an A+T-rich sequence. These could signal termination of transcription. The site of initiation for synthesis in vitro of the recA messenger RNA has been determined by analysis of the 5' nucleotide sequence of [gamma-32P]ATP-labeled transcripts. The promoter region shos a high degree of symmetry and contains sequences commonly found in recognition and binding sites for RNA polymerase.

Amino Acid Sequence↗