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Biomedical subjects

T Hirone

Publications and source records attributed to T Hirone.

34 records · Page 2Linked to original sources
PubMed1983

Synthesis of basal lamina by epidermal cells in vitro.

In epidermal cell cultures grown on type I collagen, the in vitro synthesis of basal lamina was studied by electron microscopy and indirect immunofluorescence with anti-type IV collagen antibody. The newly-formed basal lamina and type IV collagen were observed in a linear pattern beneath the epidermal cells. The observations strongly suggest that the basal lamina containing type IV collagen is newly synthesized by the epidermal cells.

Basement Membrane↗
PubMed1980

Basal lamina formation by epidermal cells in cell culture.

The formation of basal lamina in epidermal cell cultures was studied by electron microscopy. Epidermal cells were cultured on plastic dishes which were either uncoated or coated with collagen gel. The cells grew and stratified on the plastic substratum as well as on the collagen gel. While the cultures on the plastic surface showed no occurrence of hemidesmosomes and basal lamina at the interface with the plastic substratum, the cultures on the surface of the collagen gel showed development of hemidesmosomes leading to the focal occurrence of basal lamina at the interface of the collagen gel and the basal plasma membrane of basal cells. Thereafter, basal lamina occurred under the interhemidesmosomal areas in addition to the hemidesmosomal areas of the basal plasma membrane, forming a continuous layer by joining.

Basement Membrane↗
PubMed1978

Ultrastructure of giant pigment granules in lentigo simplex.

Electron microscopic observation of lentigo simplex on the sole revealed the presence in the lesion of giant pigment granules in melanocytes and keratinocytes. The giant granules were membrane-bound bodies containing electron-dense amorphous substances and less electron-dense microvesicles. It was also revealed that compound melanosomes, similar in size and shape to giant pigment granules, were present in melanocytes. Within the compound melanosomes, melanosomes showed disintergration into electron-dense fine particles concomitant with the release of less electron-dense microvesicles. These fine particles then aggregated to the mass of electrondense amorphous substances which eventually embedded the microvesicles. This evidence strongly suggests that the giant pigment granules are formed by complete degradation of melanosomes in the large compound melanosomes which can arise within melanocytes by autophagy.

Cytoplasmic Granules↗
PubMed1977

Colloid degeneration of the skin--a case report.

A patient with multiple nodules on the chin and scalp is presented. Histologically, the nodules were composed of amorphous materials which also completely filled up the whole dermis. Histochemical observation indicated that the amorphous material in the nodule was a kind of glycoprotein. Electron microscopic examination revealed that the material consists of short wavy fibrils, 60 to 100 A in diameter, and of a low electron-dense amorphous substance. Biochemical examination supported the view that the amorphous material is so-called sialomucoid. The nodules were finally diagnosed as colloid degeneration of the skin (nodular type).

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