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Biomedical subjects

T Helve

Publications and source records attributed to T Helve.

45 records · Page 3Linked to original sources
PubMed1983

Immunocompetent cells in labial salivary glands in secondary Sjögren's syndrome associated with SLE.

T and B lymphocyte (sub)populations were identified by monoclonal hybridoma antibodies (the avidin-biotin-peroxidase complex method), in the periductal lymphocyte-rich infiltrates in the labial salivary glands of 8 patients with secondary Sjögren's syndrome (2 degrees SS) associated with systemic lupus erythematosus (SLE). 59 +/- 7% and 17 +/- 3% of inflammatory round cells in situ were T3-positive and surface(SIg)- or cytoplasmic(CIg) immunoglobulin-positive, respectively. This suggests a local T lymphocyte dominance in salivary glands in 2 degrees SS associated with SLE. The local ratio of cells expressing T inducer/helper: T suppressor/cytotoxic phenotype was 3.5 +/- 0.8 (range 0.9-7.6) indicating large variations between individual patients. 46 +/- 9% of all inflammatory cells in situ were endogenous peroxidase-negative, Ia-positive cells, suggesting an active role for the locally accumulated T lymphocytes.

Adult↗
PubMed1983

Antibodies to cytoplasmic intermediate filaments in rheumatic diseases.

Antibodies to 2 types of cytoplasmic intermediate filaments (IMF)--vimentin and cytokeratin filaments--were assayed in sera from various rheumatic diseases by indirect immunofluorescence using cultured human embryonic fibroblasts and an epithelial cell line, PtK 2, as substrates. These antibodies belonged mainly to the IgM class and were detected in most of the sera. Vimentin filament antibodies of IgG or IgA class were frequent in Sjögren's syndrome (43%). Antibodies to another type of IMF, cytokeratin filaments, were also more prevalent in Sjögren's syndrome (64%) than in systemic lupus erythematosus (50%), rheumatoid arthritis (46%) and other hospital patients (8%). Our results show that intermediate filaments are a major target for autoantibodies in rheumatic diseases, especially in Sjögren's syndrome. The presence of intermediate filament IgA antibodies suggest that the stimulus for their production lies at epithelial surfaces.

Adult↗
PubMed1982

Serum pepsinogen I in rheumatic diseases. Reduced levels in Sjögren's syndrome.

Group I pepsinogens were measured by radioimmunoassay in the sera of 93 patients with rheumatic diseases and 99 controls. Patients with rheumatoid arthritis and systemic lupus erythematosus had levels of pepsinogen I similar to controls, whereas patients with Sjögren's syndrome had levels significantly lower than either controls (P less than 0.001) or patients with rheumatic diseases not associated with sicca syndrome (P less than 0.001). The concentrations of serum pepsinogen I were lower in patients with sicca syndrome alone than in those with sicca syndrome associated with some other connective tissue disease (P less than 0.05). In patients with Sjögren's syndrome, a negative correlation was found between serum concentrations of beta 2-microglobulin and pepsinogen I (r = -0.46, P less than 0.05).

Adult↗
PubMed1982

Serum beta 2-microglobulin, sialic acid, and C-reactive protein in systemic lupus erythematosus.

Serum beta 2-microglobulin (beta 2m), sialic acid and C-reactive protein (CRP) were studied in 58 patients with systemic lupus erythematosus (SLE) on 186 occasions. Serum beta 2m was significantly higher in SLE patients than in control subjects. Increased serum beta 2m levels were seen in 68% of the patients with only extrarenal manifestations of SLE, in 75% of the patients with renal manifestations but normal glomerular filtration rate, and in 100% of the patients with renal failure. Serum beta 2m levels in 12 SLE patients with associated Sjögren's syndrome were similar to those in patients without that syndrome. Serum sialic acid was also significantly increased in the SLE patients. Sixty-one (33%) of the 186 sera were positive for CRP (greater than or equal to 5 mg/l). The CRP elevation was not accompanied by recognized intercurrent infection or other superimposed cause of tissue injury and inflammation in 37 instances (61%). Under such conditions CRP was only moderately increased.

Adolescent↗
PubMed1982

Circulating DNA-antibodies in systemic lupus erythematosus.

Sera from 57 patients with systemic lupus erythematosus (SLE) were tested for DNA-antibodies by three different methods: a radioimmunological test using the Farr technique, the Crithidia luciliae immunofluorescence assay for anti-double-stranded (native) DNA (anti-ds-DNA), and a solid-phase immunoenzymatic assay for anti-single-stranded (denatured) DNA (anti-ss-DNA) of IgG and IgM classes. There was a positive correlation between the activity of the disease and the levels of ds-DNA-antibodies and IgG anti-ss-DNA. Patients with active nephritis had a higher amount of anti-ds-DNA and anti-ss-DNA of IgG class than patients with inactive nephritis (P less than 0.05 and 0.01, respectively). Patients with SLE nephritis had lower levels of ss-DNA-antibodies of IgM class than patients without nephritis (P less than 0.02). There was a positive correlation between the IgG-anti-ss-DNA/IgM-anti-ss-DNA ratio and the activity score of the disease. These results suggest that the anti-ss-DNA assay may be useful in the follow-up of SLE. Complement fixing anti-ds-DNA and the highest levels of anti-ds-DNA by Farr assay were usually associated with an active disease, especially nephritis and joint involvement.

Antibodies, Antinuclear↗
PubMed1982

Purification and characterization of a nuclear SS-B antigen.

A nuclear SS-B antigen was isolated from a saline extract of acetone powder of rabbit thymus by precipitation with ammonium sulphate, affinity chromatography with Blue Sepharose CL-6B, and preparative agarose gel electrophoresis. The mol. wt of the antigen was 68,000. Its electrophoretic mobility was similar to that of pre-albumin, and the iso-electric point was around pH 4.0. The main amino acids of the antigen were glutamic acid, leucine, lysine and alanine. Both histidine and tyrosine were also found. The purified antigen precipitated with anti-SS-B sera but not with any other reference antisera. It resembled La and Ha antigens in susceptibility to proteolytic and nucleolytic enzymes and to heat. The purified SS-B antigen, however, had a higher molecular weight than did the Ha and La antigens. The molecule could not be split into subunits with mercaptoethanol or acid. Counter-electrophoresis showed antibodies to the SS-B antigen in sera from patients with rheumatic diseases, including rheumatoid arthritis, systemic lupus erythematosus and Sjögren's syndrome, but not in amy of the control sera.

Adult↗
PubMed1978

Characterization of the DNA-synthesizing cells in rheumatoid synovial tissue.

DNA-synthesizing cells from the rheumatoid arthritis synovial tissue of 7 patients and from 5 patients with traumatic and degenerative joint lesions were studied by incubating fresh synovial tissue samples with tritiated thymidine. Labelled cells were identified using autoradiography. The tissue sections were stained with methyl green pyronine. It was found that 0.66% (range 0.38-1.4) of the cells were labelled with 3H-thymidine whereas in control materials the percentage was 0.17 (range 0-0.38). Approximately half of the labelled subsynovial cells in rheumatoid patients (range 37-67%) were small lymphocytes, according to morphological criteria. About one-tenth of the labelled cells (range 0-17%) looked like medium-sized lymphocytes, while the rest had the characteristics of fibroblasts or were unidentifiable.

Adult↗
PubMed1977

Some characteristics of RA patients with and without side effects due to gold treatment.

Gold treatment was initiated in 98 patients suffering from early-stage rheumatoid arthritis. In 30 patients side effects due to gold therapy were seen during the first follow-up year: proteinuria in 6, allergic symptoms in 23, and thrombocytopaenia in one patient. The serum IgM level was higher in patients with side effects. None of the 33 other parameters studied showed any significant differences between patients with or without side effects.

Adult↗