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Biomedical subjects

T Harada

Publications and source records attributed to T Harada.

At least 1,315 records · Page 73Linked to original sources
PubMed1968

Formation of isoamylase by Pseudomonas.

We have isolated a Pseudomonas sp. (strain SB15) which produces an isoamylase (EC 3.2.1.9). Highest yields of this enzyme were obtained when the bacterium was grown in shaken culture in a medium containing maltose, dextrin, starch, or isomaltose. Specific carbon and nitrogen sources were required for growth. The most satisfactory medium consisted of 2% maltose, 0.4% sodium glutamate, 0.3% diammonium hydrogen phosphate, and other inorganic salts. The optimal pH for enzyme production was 5 to 6. The enzyme is stable between pH 3 and 6 but is extremely labile above pH 7. It splits amylopectin completely by combined action with beta-amylase but does not attack pullulan.

Culture Media↗
PubMed1968

Formation of O-ethylhomoserine by bacteria.

Resting cells of Corynebacterium sp. E17 formed O-ethylhomoserine from ethyl alcohol for a few hours. Addition of l-homoserine greatly enhanced its formation. Thus, the formation of O-ethylhomoserine from ethyl alcohol by 27 bacteria, 6 yeasts, and 4 fungi was investigated by using growing cultures and resting cells in the presence of l-homoserine. The O-ethylhomoserine formed in the culture supernatant fluids or supernatant fluids of the reaction mixtures was identified by paper chromatography. Many organisms which were incapable of forming O-ethylhomoserine with growing cultures formed it with resting cells. The formation of O-ethylhomoserine appears to be restricted to strains of Brevibacterium, Corynebacterium, Bacillus, Mycobacterium, Nocardia, and Streptomyces.

Bacillus↗