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Biomedical subjects

T Handa

Publications and source records attributed to T Handa.

At least 127 records · Page 7Linked to original sources
PubMed1981

Surface potential of lipid membrane estimated from the partitioning of methylene blue into liposomes.

The partition of methylene blue between negatively charged phospholipid membrane and the bulk aqueous phase was measured by using visible spectroscopy in very dilute aqueous membrane suspensions, 0.05-0.3 mg of dried phospholipids in 1 mL of buffer solution. Under these experimental conditions, the turbidities of liposome systems and the overlapping of the electrical double layers of different liposomes were negligible. The positively charged probe, methylene blue, forms dimers in membrane phase, resulting in a reduction of the absorbance intensity. The surface potential of the membranes (liposomes) was calculated from the partition coefficient of the dye between the membrane and the bulk phase. The effects of charge density of the membrane and of the ionic strength on the surface potential were also studied.

Liposomes↗
PubMed1979

Urinary acid mucopolysaccharides in multiple sulfatase deficiency (mucosulfatidosis).

Urinary acid mucopolysaccharides (AMPS) excretion was investigated in a Japanese case with Multiple Sulfatase Deficiency (MSD) (Mucosulfatidosis). The patient excreted AMPS 4 to 5 times more (as carbazoluronic acid) than controls. The cellulose acetate gel electrophoresis clearly indicated two major AMPS which co-migrated with heparan sulfate and chondroitin sulfate A/C. Enzymic digestion with chondroitinase AC and ABC, and by testicular hyaluronidase plus amino sugar analysis also confirmed that our case excreted heparan sulfate and chondroitin sulfate A/C. These findings suggest that there are heterogeneities of urinary AMPS excretion among cases with MSD.

Child↗
PubMed1979

Cockayne syndrome: report of two siblings and review of literature in Japan.

Typical Cockayne syndrome was seen in a boy and his younger sister and these two cases are reported here, and reported cases of the syndrome in Japan are summarized. Both cases (an 11-year-old boy and a 7-year-old girl) had dwarfism, a senile face, retinitis pigmentosa, photosensitivity, and mental retardation. Calcium deposition in the basal ganglia was seen by CT scan. In both cases nerve conduction velocities were reduced suggesting peripheral neuropathy, but segmental demyelination on sural nerve biopsy was not demonstrated. Twenty seven cases of the syndrome has now been reported in Japan in 21 families (including the above two cases), consisting of 14 males and 12 females (the sex of one case was unknown): consanguineous marriage was confirmed in 14 families. Eleven cases in five families were siblings. The estimated ages of onset were from 1 month to 3 years, and in most cases photosensitivity was the initial symptom. Clinical manifestations were mental retardation in 25 cases (93%), dwarfism in 24 cases (89%), photosensitivity in 23 cases (85%), articular contracture in 22 cases (81%), sunken eyes in 20 cases (74%), retinitis pigmentosa in 17 cases (63%), deafness in 16 cases (59%), and intracranial calcification in 14 cases (51%). Intracranial calcification will be more often detected in future following the development of CT scanning.

Biopsy↗