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Biomedical subjects

T Haag

Publications and source records attributed to T Haag.

7 recordsLinked to original sources

[Chronic varicose pelvic veins].

After a long period of neglect, pelvic vein pathology must be recognized today as a true pathological entity. It is easy to understand how fragile the veins are and how liable to poor venous return when the anatomical, histological and functional characteristics of the venous system of the pelvis are properly understood. Furthermore this pathology has an adverse effect on peripheral and underlying venous functions. Initially revealed by transuterine hysterophlebography, the morphological and functional disorders of the pelvic veins can now be shown by color Doppler ultrasonography, which is indeed the tool of first intention for diagnosis and therapeutic assessment, being capable of displaying the variations in calibre of the veins and more particularly of establishing their flow rates. Study of pelvic vascularization has two fundamental applications for gynecology: non specific chronic, pelvic pain which represents between 15 and 20% of reasons for patients consulting, and premenstrual syndrome dominated by congestive phenomena. The indispensable accompaniment to Doppler ultrasonography for investigation of persistent pelvic pain is laparoscopy, which confirms the venous dilatation involved, assesses any associated lesions also liable to slow venous flow and offers simple and efficient methods for treatment.

Humans↗

The metal-ion-free oxidoreductase from Streptomyces aureofaciens has an alpha/beta hydrolase fold.

The crystal structure of the bromoperoxidase A2 from Streptomyces aureofaciens (ATCC 10762) has been determined by isomorphous replacement and refined to 2.05 A resolution with an R-value of 18.4%. The enzyme catalyzes the bromination of organic compounds in the presence of bromide and peroxide. The structure confirms the absence of cofactors such as metal ions or haem groups and shows the general topology of the alpha/beta hydrolase fold. The active centre is at the end of a deep pocket and includes a catalytic triad of Ser 98, Asp 228 and His 257. The active centre is connected by a narrow tunnel to a second pocket on the enzyme surface.

Amino Acid Sequence↗

Infertility due to diseased pelvic peritoneum: laparoscopic treatment.

Thirty-one patients complaining of unexplained infertility for at least 36 months and diagnosed elsewhere were reassessed laparoscopically. After staining their pelvic peritoneum with concentrated methylene blue, they presented with extensive areas of dark blue discoloration, and were diagnosed as suffering from 'diseased pelvic peritoneum'. The levels of peritoneal CA 125 were assessed. At the end of the diagnostic procedure, bipolar electrocoagulation or defocalized laser beam therapy was performed to destroy the affected peritoneal areas, and to allow peritoneal regeneration. Twenty-five pregnancies were obtained following this treatment. A detailed description is given of the diagnosis and treatment procedures.

Adult↗

Crystallization and preliminary X-ray data of bromoperoxidase from Streptomyces aureofaciens ATCC 10762.

Bromoperoxidase from Streptomyces aureofaciens ATCC 10762, a non-haem haloperoxidase, has been crystallized using the hanging drop method. Preliminary X-ray diffraction studies show that the crystals belong to the cubic space group P2(1)3 with a = 123.4 A. The asymmetric unit contains a dimer of Mr = 60,200. The crystals diffract to at least 2.3 A resolution and are suitable for crystallographic structure analysis.

Crystallization↗

Bacteremia during barium enema study.

The occurrence of bacteremia was studied in patients undergoing barium enema. Blood cultures were done on 34 patients before, during, and after the procedure using two schedules. Cultures were obtained once during the procedure in the first schedule and four times in the second. None of the cultures were positive by the first schedule, while 23% of patients studied by the second schedule had one or more positive cultures. Organisms isolated were anaerobes. The bacteremia was transient and self-limited, without serious clinical sequelae. The incidence of bacteremia during barium enema examination was statistically indistinguishable from bacteremia previously reported during colonscopy. It is concluded that antibiotic prophylaxis is not indicated in most patients undergoing colonic diagnostic procedures. Prophylaxis in selected high-risk patients requires further study.

Adult↗