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Biomedical subjects

T Golding

Publications and source records attributed to T Golding.

5 recordsLinked to original sources

Childhood peptic ulcer disease in Jamaica.

Thirty-one children with peptic ulcer disease (PUD) were diagnosed in two major hospitals in Jamaica between 1979 and 1988. There were 21 girls and 10 boys with a mean age of 9 years. There were 26 duodenal ulcers and 5 gastric ulcers. Five patients (19 per cent) with a duodenal ulcer had a family history of peptic ulcer. Recurrent abdominal pain was the predominant presenting feature in 68 per cent and pain was present for a mean of 20 months. Bleeding occurred in seven patients (23 per cent). Twenty-nine patients were successfully treated medically. Two with duodenal ulcers required surgery. The majority of patients have remained well after a mean follow-up of 14 months. Although uncommon in children peptic ulcers should be considered in patients with recurrent abdominal pain. The majority will respond to medical treatment.

Adolescent

Bimodal nuclear estrogen receptor binding: synchrony in athymic mouse uterus and human breast tumor xenografts.

Human MCF-7 tumors were transplanted into ovariectomized female athymic nude mice supplemented with estradiol pellets. Ten days after hormone pellet removal, the animals were treated with 10 micrograms/kg estradiol, and the nuclear estrogen receptor (ERn) profile was assessed by the exchange assay. The pattern in the tumors was qualitatively similar to that in the uterus. A bimodal pattern of ERn was seen, with peaks at 1 and 8 h. Further biochemical analysis of uterine samples showed that both peaks were comprised of similar levels of salt-resistant ERn forms. Scatchard plot analysis of estradiol binding demonstrated high affinity receptors (Kd = 0.73-0.86 nM) as components of both peaks. In the ovariectomized adult rat there was also a bimodal pattern of ERn 1 and 13-14 h after the injection of 20 micrograms/kg estradiol. Direct hormone stimulation of the uterus was achieved with intraluminal (IL) injection of estradiol. IL injections of estradiol (100-800 pg/horn) stimulated uterine DNA synthesis compared to IL saline injections in the contralateral horn. IL injection of 200 pg/horn estradiol resulted in a bimodal pattern of ERn at 3 and 9 h. These data indicate that a bimodal pattern of ERn is present in estrogen target tissues exhibiting a growth response.

Animals